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Human Expandable Base Panel (15c)

 

A 15-color expandable panel for human immune cell phenotyping, enabling detailed characterization of monocytes, macrophages, NK and NKT cells, T cells, B cells, dendritic cells, basophils, and ILCs. The panel is designed to keep channels with broad conjugate availability open, allowing seamless customization. Several strategically placed drop-in/open spots enable the addition of extra markers or conjugates, supporting straightforward expansion up to 23 colors without disrupting the core panel design.

 

To view a high-resolution version of the data shown below, download our PDF.

 

• Instrument used for optimization: 5-laser Cytek® Aurora (UV, V, B, YG, R)
• Ordering information: Please contact us to order the listed reagents through a promotional bundle. Each bundle includes ancillary reagents (e.g. Fc block, staining buffer, compensation beads) in addition to the fluorophore-conjugated antibodies needed for the experiment.
• Product Name: Human Expandable Base Panel (15c)

 

Marker, Fluorophore, and Clone List:

 

Product	Clone
Spark PLUS UV395™ anti-human CD8	RPA-T8	View Product
Brilliant Violet 510™ anti-human CD3	SK7	View Product
Brilliant Violet 570™ anti-human CD20	2H7	View Product
Brilliant Violet 750™ anti-human CD56 (NCAM)	5.1H11	View Product
Spark Blue™ 550 anti-human CD14	63D3	View Product
Spark Blue™ 574 anti-human CD45	2D1	View Product
PerCP/Cyanine5.5 anti-human CD16	B73.1	View Product
PerCP/Fire™ 806 anti-human CD4	SK3	View Product
Spark YG™ 593 anti-human CD19	HIB19	View Product
PE/Fire™ 700 anti-human CD25	M-A251	View Product
PE/Fire™ 810 anti-human HLA-DR	L243	View Product
Spark NIR™ 685 anti-human CD123	S18016F	View Product
APC/Fire™ 750 anti-human CD11c	Bu15	View Product
APC/Fire™ 810 anti-human CD127 (IL-7Rα)	A019D5	View Product
Zombie NIR™ Fixable Viability Kit		View Product
Human TruStain FcX™ (Fc Receptor Blocking Solution)		View Product
Cell Staining Buffer		View Product
True-Stain Monocyte Blocker™		View Product
Compensation Beads		View Product

 

15-Color Base Panel

Representative data is shown below. Human PBMCs were stained with the 15-color expandable panel, acquired on a 5-laser (UV/V/B/YG/R) Cytek® Aurora, and analyzed using CytoScribe™ software.

 

 

23-Color Expanded Panel

Representative data from an example expanded configuration is shown below. The dyes listed below represent an incomplete list of potential fluorophores that can be incorporated into the open channels based on experimental needs.

 

Human PBMCs were stained with the an expanded, 23-color panel, acquired on a 5-laser (UV/V/B/YG/R) Cytek® Aurora, and analyzed using CytoScribe™ software.

 

Fluorophore Drop-in Options
Spark UV™ 387	Spark Violet™ 423	Spark Blue™ 515	PE/Cyanine5
StarBright™ UltraViolet 510	Spark PLUS V450™	FITC	PE/Cyanine7
StarBright™ UltraViolet 575	Spark PLUS V475™	PerCP	Spark YG™ 581
StarBright™ UltraViolet 605	Spark Violet™ 538	PerCP/Fire™ 780	PE/Fire™ 744
StarBright™ UltraViolet 665	Brilliant Violet 605™	PE	APC
StarBright™ UltraViolet 740	Brilliant Violet 650™	PE/Dazzle™ 594	Alexa Fluor® 647
StarBright™ UltraViolet 795	Brilliant Violet 711™	Alexa Fluor® 594	Alexa Fluor® 700
Brilliant Violet 421™	Brilliant Violet 785™	PE/Fire™ 640	Spark Red™ 718