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Human T Cell Differentiation and CCRs (27c)

 

A 27-color panel focused on comprehensive T cell subset phenotyping based on chemokine receptor expression. Panel optimization was performed using a 5-laser Cytek Aurora.

 

• Instrument used for optimization: 5-laser Cytek® Aurora (UV, V, B, YG, R)
• Ordering information: Please contact us to order the listed reagents through a promotional bundle. Each bundle includes ancillary reagents (e.g. Fc block, staining buffer, compensation beads) in addition to the fluorophore-conjugated antibodies needed for the experiment.
• Product Name: Human T Cell Differentiation and CCRs (27c)

 

Marker, Fluorophore, and Clone List:

 

Product	Clone
Brilliant Violet 421™ anti-human CD197 (CCR7)	G043H7	View Product
Pacific Blue™ anti-human CD28	CD28.2	View Product
Brilliant Violet 510™ anti-human CD3	OKT3	View Product
Brilliant Violet 570™ anti-human CD127 (IL-7Rα)	A019D5	View Product
Brilliant Violet 605™ anti-human CD183 (CXCR3)	G025H7	View Product
Brilliant Violet 650™ anti-human CD196 (CCR6)	G034E3	View Product
Brilliant Violet 711™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	View Product
Brilliant Violet 750™ anti-human CD185 (CXCR5)	J252D4	View Product
Brilliant Violet 785™ anti-human CD161	HP-3G10	View Product
FITC anti-human TCR Vα7.2	3C10	View Product
Spark Blue™ 550 anti-human CD8	SK1	View Product
Spark Blue™ 574 anti-human CD45	2D1	View Product
PerCP/Cyanine5.5 anti-human CD195 (CCR5)	J418F1	View Product
PE anti-human CD279 (PD-1)	A17188A	View Product
Spark YG™ 581 anti-human CD4	SK3	View Product
PE/Dazzle™ 594 anti-human CD95 (Fas)	DX2	View Product
PE/Fire™ 640 anti-human CD38	S17015F	View Product
PE/Cyanine5 anti-human CD25	M-A251	View Product
PE/Fire™ 700 anti-human TCR γ/δ	B1	View Product
PE/Cyanine7 anti-human CD39	A1	View Product
PE/Fire™ 810 anti-human CD194 (CCR4)	L291H4	View Product
APC anti-human CCR10	6588-5	View Product
Spark NIR™ 685 anti-human CD45RA	HI100	View Product
Alexa Fluor® 700 anti-human CD19	HIB19	View Product
Alexa Fluor® 700 anti-human CD14	63D3	View Product
APC/Fire™ 750 anti-human CD27	O323	View Product
APC/Fire™ 810 anti-human HLA-DR	L243	View Product
Zombie NIR™ Fixable Viability Kit	Viability	View Product
Human TruStain FcX™ (Fc Receptor Blocking Solution)		View Product
Cell Staining Buffer		View Product
True-Stain Monocyte Blocker™		View Product
Tandem Stabilizer		View Product
Compensation Beads		View Product

 

Representative data:

 

Human PBMCs from a healthy donor stained with the Human T Cell Differentiation and CCRs (27c) panel. Samples were acquired on a 5-laser (UV/V/B/YG/R) Cytek® Aurora using manufacturer recommended standard instrument settings.