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Human Regulatory T Cell Phenotyping (24c)

 

A 24-color panel to examine the subset of CD4+ T cells that regulate the immune response and prevent autoimmunity. Panel optimization was performed using a 5-laser Cytek Aurora.

 

• Instrument used for optimization: 5-laser Cytek® Aurora (UV, V, B, YG, R)
• Ordering information: Please contact us to order the listed reagents through a promotional bundle. Each bundle includes ancillary reagents (e.g. Fc block, staining buffer, compensation beads) in addition to the fluorophore-conjugated antibodies needed for the experiment.
• Product Name: Human T Cell Differentiation and CCRs (27c)

 

Marker, Fluorophore, and Clone List:

 

Product	Clone
Brilliant Violet 421™ anti-human CD226 (DNAM-1)	11A8	View Product
Pacific Blue™ anti-human CD28	CD28.2	View Product
Brilliant Violet 510™ anti-human CD3	OKT3	View Product
Brilliant Violet 570™ anti-human CD127 (IL-7Rα)	A019D5	View Product
Brilliant Violet 605™ anti-human CD152 (CTLA-4)	BNI3	View Product
Brilliant Violet 650™ anti-human CD366 (Tim-3)	F38-2E2	View Product
Brilliant Violet 711™ anti-human CD134 (OX40)	Ber-ACT35	View Product
Brilliant Violet 785™ anti-human CD154 (CD40L)	24-31	View Product
FITC anti-human CD45RA	HI100	View Product
Spark Blue™ 550 anti-human CD8	SK1	View Product
Spark Blue™ 574 anti-human CD45	2D1	View Product
PE anti-human CD198 (CCR8)	S19016H	View Product
Spark YG™ 581 anti-human CD4	SK3	View Product
PE/Dazzle™ 594 anti-human CD279 (PD-1)	EH12.2H7	View Product
PE/Fire™ 640 anti-human TIGIT (VSTM3)	A15153G	View Product
PE/Cyanine5 anti-human CD357 (GITR)	621	View Product
PE/Fire™ 700 anti-human CD25	M-A251	View Product
PE/Cyanine7 anti-human CD197 (CCR7)	G043H7	View Product
PE/Fire™ 810 anti-human CD39	A1	View Product
Alexa Fluor® anti-human FOXP3	206D	View Product
Alexa Fluor® 700 anti-human CD14	63D3	View Product
Alexa Fluor® 700 anti-human CD19	SJ25C1	View Product
APC/Fire™ 750 anti-human CD27	O323	View Product
APC/Fire™ 810 anti-human HLA-DR	L243	View Product
Zombie NIR™ Fixable Viability Kit	Viability	View Product
Human TruStain FcX™ (Fc Receptor Blocking Solution)		View Product
True-Nuclear™ Transcription Factor Buffer Set		View Product
Cell Staining Buffer		View Product
True-Stain Monocyte Blocker™		View Product
Tandem Stabilizer		View Product
Compensation Beads		View Product

 

Representative data:

 

Human PBMCs from a healthy donor stained with the Human Regulatory T cell Phenotyping (24c) panel. Samples were acquired on a 5-laser (UV/V/B/YG/R) Cytek® Aurora using manufacturer recommended standard instrument settings.