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Newly Validated Antibodies for IHC and ICC

BioLegend offers extensive and diverse antibodies with one of the largest clone libraries in the industry. With two decades of antibody crafting experience, we offer high-quality antibodies validated for several applications including western blotting, flow cytometry, and microscopy. Within microscopy, immunohistochemistry (IHC) and immunocytochemistry (ICC) can drive discovery in research fields including oncology, immunology, and neurology. With this in mind, we are continuously working to add further applications to existing products. Read below to learn about some of our newly validated antibodies for IHC and ICC applications.

 

Recombinant antibodies, as denoted by Rec or RecM in the clone name, demonstrate specificity, reproducibility and renewability via animal-free production.

 

View our IHC/ICC applications webpage for protocols and a complete list of microscopy-validated antibodies.

 

Specificity Format Clone
anti-AMPKα Phospho (Thr172) A647 A20017A
anti-Avi-tag A488, A594, A647 W19258A
anti-BG-4 PURE 17-206.RecM1
anti-CD31 (PECAM-1) PURE, A647 JC70
anti-CD340 (ErbB2/HER-2) PURE A20145B
anti-CD340 (ErbB2/HER-2) PURE W22266D
anti-c-Fos A647 7D6-A9-D4
anti-c-Fos Phospho (Ser32) PURE, A647 A22009C
anti-CREB1 Phospho (Ser133) PURE A22026B
anti-Giantin PURE, A488 Poly23A01
anti-Histone H3 PURE 1B1-B2.RecM1
anti-IBA1 PURE A19290E
anti-MDM2 PURE W23003F
anti-Nestin PURE Poly22A17
anti-Neurofilament H (NF-H), Nonphosphorylated SPKYG_570 SMI 32
anti-Neurofilament H (NF-H), Phosphorylated SPKYG_570 SMI 31
anti-NF-kB p65 PURE A21012A
anti-NF-κB p65 Phospho (Ser529) PURE A21012B
anti-NOS3 (eNOS) PURE W22110B
anti-Olig2 PURE W21023A
anti-Oligodendrocyte Marker O1 A488, A594, A647 O1
anti-Pan-Cytokeratin A488, A594, A647 AE-1/AE-3
anti-PTEN Phospho (Ser380) PURE, A488, A647 A22005D
anti-RBFOX3 (NeuN) A488, A647 W18230A
anti-SOX17 Recombinant PURE, A488, A647 O92B6.Rec
anti-Tau Phospho (Thr231) PURE A23009A
anti-TMEM119 (Extracellular) A488, A594, A647 A16075D
anti-Vimentin A488 W16220A
anti-α Smooth Muscle Actin PURE, A488, A647 1A4
anti-β-Amyloid, 1-16 Recombinant PURE 6E10.Rec
anti-β-Amyloid, 17-28 PURE A18104E
anti-β-Catenin non-phospho (active) (S33/S37/T41) PURE W22009A
anti-γ-Catenin PURE W22004E
 

Microscopy image of Histone H3 staining.

 

IHC staining of Purified anti-Histone H3 (C-terminus) (clone 1B1-B2.recM1) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-Histone H3 (C-terminus) Recombinant (clone 1B1-B2.recM1) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. The tissue was subsequently co-stained with Alexa Fluor® 488 anti-Vimentin (clone O91D3) (green) and nuclei were counterstained with DAPI (Cat. No. 422801) (blue). Images were captured with a 40X objective and merged. Scale bar: 50 μm

 

 

IHC staining of Purified anti-BG-4 Recombinant antibody (clone 17-206.RecM1) on formalin-fixed paraffin-embedded human antrum (stomach) tissue. The tissue was incubated with primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining. The images were captured using a 10X (panel A) and 40X (panel B) objective. Scale bar: 50 μm

 

 

IHC staining of Alexa Fluor® 647 anti-Pan Cytokeratin (clone AE-1/AE-3) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated either without (panel A) or with (panel B) Alexa Fluor® 647 anti-Pan Cytokeratin (clone AE-1/AE-3). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 μm

 

 

HeLa cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Alexa Fluor® 488 anti-Giantin (clone Poly23A01) (panel A). Cells were also co-stained with Alexa Fluor® 647 anti-Tubulin-α (clone 10D8) (panel B) and nuclei were counterstained with DAPI (Cat. No. 422801) (panel C). Scale bar: 50 µm

 

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