Direct-Blot™

There are two main methods of analyzing your western blot: chemiluminescence or fluorescent detection. In chemiluminescence detection, protein detection is accomplished by using antibodies conjugated to an enzyme such as horseradish peroxidase (HRP) which catalyzes a substrate to emit light. Typically, users perform indirect detection with a primary antibody to bind the target protein and a secondary antibody conjugated to HRP. However, unless the signal needs to be amplified, there is no advantage to using a two-step indirect method. In direct detection, primary antibody is conjugated to HRP and removes the need for a 2nd step procedure.

Our Direct-Blot™ antibodies are horseradish peroxidase (HRP)-conjugated and eliminate the need for a secondary antibody and additional processing steps. By removing several steps from the workflow, they shorten the protocol and help you get your results faster. Our scientists have carefully developed and tested these antibodies to ensure they provide high sensitivity and excellent stability. We are happy to provide Direct-Blot™ primary antibodies directly conjugated to HRP to help you simplify and reduce the cost of your experiment.

Follow our Direct-Blot™ protocol and learn more about Direct-Blot™ antibodies.