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Human General Phenotyping (26c)

 

A 26-color panel to examine major immune cell subsets from human peripheral blood. Panel optimization was performed using a 5-laser Cytek Aurora.

 

• Instrument used for optimization: 5-laser Cytek® Aurora (UV, V, B, YG, R)
• Ordering information:
  This panel is now available as an off-the-shelf catalog product. View the TDS here. 
  To order the reagents listed below as a promotional bundle, please contact us. Each bundle includes ancillary reagents (e.g. Fc block, staining buffer, compensation beads) in addition to the fluorophore-conjugated antibodies needed for the experiment.
• Product Name: Human General Phenotyping (26c)

 

Marker, Fluorophore, and Clone List:

 

Product	Clone
Brilliant Violet 421™ anti-human CD197 (CCR7)	G043H7	View Product
Pacific Blue™ anti-human CD20	2H7	View Product
Brilliant Violet 510™ anti-human IgM	MHM-88	View Product
Brilliant Violet 570™ anti-human CD3	UCHT1	View Product
Brilliant Violet 605™ anti-human CD16	3G8	View Product
Brilliant Violet 650™ anti-human CD28	CD28.2	View Product
Brilliant Violet 711™ anti-human CD38	HIT2	View Product
Brilliant Violet 750™ anti-human CD56 (NCAM)	5.1H11	View Product
Brilliant Violet 785™ anti-human CD279 (PD-1)	EH12.2H7	View Product
Alexa Fluor® 488 anti-human CD45RA	HI100	View Product
Spark Blue™ 550 anti-human CD14	63D3	View Product
Spark Blue™ 574 anti-human CD4	SK3	View Product
PerCP/Cyanine5.5 anti-human CD8	SK1	View Product
PE anti-human TCR γ/δ	B1	View Product
Spark YG™ 581 anti-human IgD	W18340F	View Product
PE/Dazzle™ 594 anti-human CD141 (Thrombomodulin)	M80	View Product
PE/Fire™ 700 anti-human CD25	M-A251	View Product
PE/Cyanine7 anti-human CD11c	3.9	View Product
PE/Fire™ 810 anti-human HLA-DR	L243	View Product
APC anti-human CD127 (IL-7Rα)	A019D5	View Product
Alexa Fluor® 647 anti-human CD1c	L161	View Product
Spark NIR™ 685 anti-human CD19	HIB19	View Product
Alexa Fluor® 700 anti-human CD123	6H6	View Product
Zombie NIR™ Fixable Viability Kit	Viability	View Product
APC/Fire™ 750 anti-human CD45	2D1	View Product
APC/Fire™ 810 anti-human CD27	O323	View Product
Human TruStain FcX™ (Fc Receptor Blocking Solution)		View Product
Cell Staining Buffer		View Product
True-Stain Monocyte Blocker™		View Product
Tandem Stabilizer		View Product
Compensation Beads		View Product

 

Representative data:

 

Human PBMCs were stained with the Human General Phenotyping (26-color) panel and acquired on a 5-laser (UV/V/B/YG/R) Cytek® Aurora.