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Human Myeloid Phenotyping (27c)

 

A 27-color panel phenotyping human myeloid cells, enabling detailed characterization of monocytes, macrophages, and dendritic cell subsets.

 

• Instrument used for optimization: 5-laser Cytek® Aurora (UV, V, B, YG, R)
• Ordering information: Please contact us to order the listed reagents through a promotional bundle. Each bundle includes ancillary reagents (e.g. Fc block, staining buffer, compensation beads) in addition to the fluorophore-conjugated antibodies needed for the experiment.
• Product Name: Human Myeloid Phenotyping (27c)

 

Marker, Fluorophore, and Clone List:

 

Product	Clone
Brilliant Violet 421™ anti-human CD272 (BTLA)	MIH26	View Product
Pacific Blue™ anti-human CD16	3G8	View Product
Brilliant Violet 510™ anti-human CD3	SK7	View Product
Brilliant Violet 605™ anti-human CD80	2D10	View Product
Brilliant Violet 650™ anti-human CD1c	L161	View Product
Brilliant Violet 711™ anti-human CD163	GHI/61	View Product
Brilliant Violet 785™ anti-human CD40	5C3	View Product
FITC anti-human CD64	10.1	View Product
Spark Blue™ 550 anti-human CD14	63D3	View Product
Spark Blue™ 574 anti-human CD45	2D1	View Product
PerCP/Cyanine5.5 anti-human CD45RA	HI100	View Product
PerCP/Fire™ 806 anti-mouse/human CD11b	M1/70	View Product
PE anti-human CD32	FUN-2	View Product
Spark YG™ 593 anti-human CD19	HIB19	View Product
PE/Dazzle™ 594 anti-human CX3CR1	2A9-1	View Product
PE/Fire™ 640 anti-human CD86	IT2.2	View Product
PE/Cyanine5 anti-human CD26	BA5b	View Product
PE/Fire™ 700 anti-human CD38	S17015A	View Product
PE/Cyanine7 anti-human CD85k (ILT3)	ZM4.1	View Product
PE/Fire™ 810 anti-human HLA-DR	L243	View Product
Alexa Fluor® 647 anti-human CD141 (Thrombomodulin)	M80	View Product
APC anti-human CD172a/b (SIRPα/β)	SE5A5	View Product
Spark NIR™ 685 anti-human CD123	S18016F	View Product
Spark Red™ 718 anti-human CD56 (NCAM)	5.1H11	View Product
APC/Fire™ 750 anti-human CD11c	S-HCL-3	View Product
APC/Fire™ 810 anti-human CD15 (SSEA-1)	W6D3	View Product
Zombie NIR™ Fixable Viability Kit	Viability	View Product
Human TruStain FcX™ (Fc Receptor Blocking Solution)		View Product
Cell Staining Buffer		View Product
True-Stain Monocyte Blocker™		View Product
Tandem Stabilizer		View Product
Compensation Beads		View Product

 

Representative data:

 

Human PBMCs from a healthy donor stained with the Human Myeloid Phenotyping (27c) panel. Samples were acquired on a 5-laser (UV/V/B/YG/R) Cytek® Aurora using manufacturer recommended standard instrument settings.