Loading...

Intracellular Staining Permeabilization Wash Buffer (10X)

Pricing & Availability
Regulatory Status
RUO
Other Names
Perm/Wash, Permeabilization Buffer
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
421002 100 mL 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Intracellular Staining Permeabilization Wash Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Intracellular Staining Permeabilization Wash Buffer is used to permeabilize cells following fixation with Intracellular Staining Fixation Buffer (Cat. No. 420801). It is supplied as a 10X solution and should be diluted in deionized water prior to use. Intracellular Staining Permeabilization Wash Buffer has been formulated to have minimal effects on cells, reduce non-specific staining and enhance the signal to noise ratio. It can be used for antibody dilutions and cell washing during intracellular staining.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
The Intracellular Staining Perm wash buffer solution should be stored between 2°C and 8°C. Do not freeze.
Application

ICC, ICFC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

For use in permeabilization, dilute Intracellular Staining Permeabilization Wash Buffer (10X) to 1X in DI water. Resuspend fixed cells in diluted Intracellular Staining Permeabilization Wash Buffer and centrifuge at 350 xg for 5-10 minutes, and repeat the process twice. It is recommended that the reagent be titrated for optimal performance for each application. Please see "Intracellular Cytokine Staining Protocol" on BioLegend's website for more details.

Application Notes

The Intracellular Staining Permeabilization Wash Buffer (10X) may have crystallization or precipitation observed when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is heavy precipitation observed after dilution to 1X working solution, the buffer can be filtered to clarify the solution.

Application References

(PubMed link indicates BioLegend citation)
  1. Smeltz RB. 2007. J. Immunol. 178:4786.
  2. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  3. del Rio ML, et al. 2011. Transplantation. 92:1085. PubMed
  4. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
  5. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  6. Xiao Z, et al. 2013. Mol Immunol. 56:423. PubMed
  7. Kusner LL, et al. 2014. PLoS One. 9:102231. PubMed
  8. Ni PP, et al. 2014. J Immunol. 193:1778. PubMed
Product Citations
  1. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  2. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  3. Kim S, et al. 2020. Development. 147:00:00. PubMed
  4. Dumauthioz N, et al. 2020. Cell Mol Immunol. . PubMed
  5. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  6. Arbues A, et al. 2020. PLoS Pathog. 16:e1008312. PubMed
  7. Kim J, et al. 2021. Cytometry A. 99:807. PubMed
  8. Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed
  9. Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed
  10. Nozaki K, et al. 2022. Nature. 606:960. PubMed
  11. Shemesh A, et al. 2022. J Exp Med. 219: . PubMed
  12. Yin XY, et al. 2023. Neural Regen Res. 18:213. PubMed
  13. Blanas A, et al. 2022. iScience. 25:104719. PubMed
  14. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
  15. Gong YY, et al. 2022. Cancer Res Commun. 2:842. PubMed
  16. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
  17. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  18. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  19. Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed
  20. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  21. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  22. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  23. Chan L, et al. 2022. Cells. 12: . PubMed
  24. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  25. Qin S, et al. 2023. Acta Pharm Sin B. :. PubMed
  26. Sun L, et al. 2023. Mol Med Rep. 27: . PubMed
  27. Di Benedetto C, et al. 2023. Cancers (Basel). 15: . PubMed
  28. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  29. Xiao JF, et al. 2022. Mol Cancer Res. 20:637. PubMed
  30. Shen JZ, et al. 2022. Mol Cell. 82:1123. PubMed
  31. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  32. Zhang H, et al. 2022. J Exp Med. 219:. PubMed
  33. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  34. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  35. Chen R, et al. 2022. J Mol Cell Biol. 14:. PubMed
  36. Tang J, et al. 2022. Sci Immunol. 7:eadd4853. PubMed
  37. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed
  38. Hajnik RL, et al. 2022. Sci Transl Med. 14:eabq1945. PubMed
  39. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  40. Li L, et al. 2022. Cancer Immunol Res. 10:1475. PubMed
  41. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  42. Stokes JV, et al. 2022. Curr Protoc. 2:e584. PubMed
  43. Jia DJ, et al. 2022. Gut Microbes. 14:2145843. PubMed
  44. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  45. Kodama N, et al. 2023. Front Nutr. 10:1081263. PubMed
  46. Mohamed GA, et al. 2023. Breast Cancer Res. 25:23. PubMed
  47. Varun K, et al. 2023. EBioMedicine. 90:104516. PubMed
  48. Wang YH, et al. 2023. Int J Mol Sci. 24:. PubMed
  49. Deng G, et al. 2023. Cancers (Basel). 15:. PubMed
  50. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  51. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
  52. Ruan H, et al. 2023. Acta Pharm Sin B. 13:2202. PubMed
  53. Zhang TR, et al. 2023. Pharmaceuticals (Basel). 16:. PubMed
  54. Komoto S, et al. 2023. Cancers (Basel). 15:. PubMed
  55. Hanga MP, et al. 2021. Biotechnol Bioeng. 118:3175. PubMed
  56. Peng, Q., Jiang, P., et al. 2025. Gut Microbes. 17(1),2541028. PubMed
  57. Zhang, N., Li, C., et al. 2025. Cell Death Dis. 17(1),115. PubMed
  58. Lebedeva IV, et al. 2021. Cell Death Dis. 12:770. PubMed
  59. Zhang L, et al. 2021. J Immunol. 207:590. PubMed
  60. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  61. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  62. Glaser K, et al. 2015. Immunol Lett. 166: 19-27. PubMed
  63. Kusner L, et al. 2014. PLoS One. 9:102231. PubMed
  64. Khayrullina G, et al. 2020. Skelet Muscle. 10:16. PubMed
  65. Spitzer SO, et al. 2019. Neuron. 101:459. PubMed
  66. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  67. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  68. van Vloten JP, et al. 2022. J Immunother Cancer. 10:. PubMed
  69. Huang X, et al. 2019. Cancer Immunol Res. 1.388888889. PubMed
  70. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  71. Jacob A, et al. 2020. Nature Protocols. 14(12):3303-3332.. PubMed
  72. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  73. Stewart CA, et al. 2020. bioRxiv. . PubMed
  74. Stewart CA, et al. 2021. J Thorac Oncol. Online ahead of prin. PubMed
  75. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  76. Poli V, et al. 2021. iScience. 24:103256. PubMed
  77. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  78. Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed
  79. Zhang S, et al. 2022. Cell Death Dis. 13:844. PubMed
  80. Gärtner F, et al. 2021. Front Immunol. 12:745132. PubMed
  81. Gao F, et al. 2021. Front Cell Infect Microbiol. 10:593805. PubMed
  82. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  83. Martinez-Usatorre A, et al. 2020. Front Immunol. 11:340. PubMed
  84. Martinez–Usatorre A, et al. 2019. Cancer Immunol Res. 0.995138889. PubMed
  85. Goyal S, et al. 2017. Sci Rep. . 10.1038/s41598-017-18046-x. PubMed
  86. Tsai CY, et al. 2022. J Biomed Sci. 29:47. PubMed
  87. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  88. Shinde MY, et al. 2017. J Biol Chem. 292:18240. PubMed
  89. Haryadi L, et al. 2019. Vet World. 12:1529. PubMed
  90. Ghose J, et al. 2018. Oncoimmunology. 7:e1486948. PubMed
  91. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  92. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  93. Herda S, et al. 2021. Int J Cancer. 148:3097. PubMed
  94. Lau H, et al. 2021. Int J Mol Sci. 22:. PubMed
  95. Zhou H, et al. 2022. J Clin Invest. 132:. PubMed
  96. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  97. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  98. Hendricks A, et al. 2021. Front Oncol. 11:646885. PubMed
  99. Shamsi F, et al. 2021. Nat Metab. 3:485. PubMed
  100. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  101. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  102. Sohrabi Y, et al. 2020. Front Immunol. 11:353. PubMed
  103. Martinez–Usatorre A, et al. 2018. J Immunol. 201:792. PubMed
  104. Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed
  105. Lau H, et al. 2021. Xenotransplantation. 28:e12667. PubMed
  106. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  107. Ma Q, et al. 2022. Nat Commun. 13:4142. PubMed
  108. Datta A, et al. 2022. J Fungi (Basel). 8:. PubMed
  109. Clancy–Thompson E, et al. 2018. Cancer Immunol Res. 1.308333333. PubMed
  110. Xiao Z, et al. 2013. Mol Immunol. 56:423. PubMed
  111. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  112. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  113. Zhang X, et al. 2022. Theranostics. 12:1621. PubMed
  114. Berical A, et al. 2022. Nat Commun. 13:4270. PubMed
  115. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  116. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  117. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  118. Pearce V, et al. 2015. J Immunol. 195: 3206 - 3217. PubMed
  119. Baek J, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.02.011. PubMed
  120. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  121. Rodriguez S, et al. 2020. Xenotransplantation. 27:e12554. PubMed
  122. Lownik JC, et al. 2019. J Immunol. 202:664. PubMed
  123. Lau H, et al. 2020. Xenotransplantation. 27:e12555. PubMed
  124. Mkhikian H, et al. 2022. Nat Aging. 2:231. PubMed
  125. Li J, et al. 2022. J Tissue Eng. 13:20417314221127908. PubMed
  126. van Duijn A, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  127. Jakic B, et al. 2021. Cell Death Dis. 12:187. PubMed
  128. Ouyang W, et al. 2021. Invest Ophthalmol Vis Sci. 62:25:00. PubMed
  129. Nausch N, et al. 2017. PLoS Negl Trop Dis. 11(2):e0005415. PubMed
  130. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  131. Breznik JA, et al. 2018. Physiol Rep. 6:e13937. PubMed
  132. Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  133. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  134. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  135. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  136. Alvandi Z, et al. 2022. J Am Heart Assoc. 11:e023695. PubMed
  137. Li Q, et al. 2017. Front Immunol. 0.797916667. PubMed
  138. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  139. Gunasena M, et al. 2022. Sci Rep. 12:12377. PubMed
  140. Gong C, et al. 2021. Mol Cell. 81:4059. PubMed
  141. Chen DL, et al. 2021. Mol Cancer. 20:166. PubMed
  142. Kim S, et al. 2020. Development. . PubMed
  143. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  144. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  145. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  146. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  147. Bajpai G, et al. 2018. Nat Med. 24:1234. PubMed
  148. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  149. Wilmes A, et al. 2017. Toxicol In Vitro. 10.1016/j.tiv.2017.07.023. PubMed
  150. Hayes D, et al. 2022. JCI Insight. 7:. PubMed
  151. Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed
  152. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  153. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  154. Wen T, et al. 2022. Cancer Immunol Res. 10:162. PubMed
  155. Ma K, et al. 2022. iScience. 25:104347. PubMed
  156. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  157. Haswell JR, et al. 2021. PLoS One. 16:e0252848. PubMed
  158. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  159. Lau H, et al. 2020. Islets. 41:12. PubMed
  160. Lau H, et al. 2020. PLoS One. 15:e0243506. PubMed
  161. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  162. Dong M, et al. 2022. J Immunother Cancer. 10:. PubMed
  163. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  164. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  165. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  166. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  167. Park SR, et al. 2020. Cell Rep. 32:108077. PubMed
  168. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  169. Spiliopoulou P, et al. 2022. Mol Cancer Ther. . PubMed
  170. Tintos-Hernandez JA, et al. 2021. Brain Commun. 3:fcab215. PubMed
  171. Koutník J, et al. 2022. Cell Commun Signal. 20:54. PubMed
  172. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  173. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  174. Gao J, et al. 2022. Front Immunol. 13:955812. PubMed
  175. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  176. DiMuzio JM, et al. 2021. Vaccine X. 8:100098. PubMed
  177. Lee G, et al. 2021. Viruses. 13: . PubMed
  178. Lei L, et al. 2021. Front Cell Dev Biol. 659744:9. PubMed
  179. Frafjord A, et al. 2020. Scand J Immunol. e12889:92. PubMed
  180. Lownik JC, et al. 2020. Biochem Biophys Rep. 24:100803. PubMed
  181. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  182. Sun X, et al. 2017. Microbes Infect. 10.1016/j.micinf.2017.08.007. PubMed
  183. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  184. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  185. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  186. Huang T, et al. 2022. Nat Commun. 13:3489. PubMed
  187. Hernandez AA, et al. 2020. J Immunol. 205:2806. PubMed
  188. Su Y, et al. 2020. Cell. 1479:183. PubMed
  189. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  190. Mcgaw C, et al. 2022. Nat Commun. 13:2833. PubMed
  191. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  192. van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed
  193. Wang X, et al. 2022. Elife. 11:. PubMed
  194. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  195. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  196. Zheng L, et al. 2020. Clin Cancer Res. 26:3694. PubMed
  197. Witt G, et al. 2020. Cell Biol Toxicol. . PubMed
  198. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  199. Giadone RM, et al. 2018. Amyloid. 25:148. PubMed
  200. Gebremeskel S, et al. 2017. Cancer Immunol Res. 0.9625. PubMed
  201. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  202. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  203. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  204. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  205. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  206. Luvanda MK, et al. 2021. J Fungi (Basel). 7:. PubMed
  207. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  208. Assadi G, et al. 2016. PLoS One. 11:e0168276. PubMed
  209. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  210. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  211. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08.26.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account