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Purified anti-DNER Antibody

Pricing & Availability
Clone
W23166E (See other available formats)
Regulatory Status
RUO
Other Names
Delta and Notch-like epidermal growth factor-related receptor (DNER), BET, UNQ26/PRO299, HE60
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W23166E_PURE_DNER_IHC-P_060925
IHC staining of DNER was performed on formalin-fixed paraffin-embedded human cerebellum tissue following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704). The tissue sections were incubated with Alexa Fluor® 647 secondary antibody only (panel A) or with anti-DNER (clone W23166E) (panel B). Nuclei were counterstained with DAPI (Cat. No. 422801) (overlay in panel B). Scale Bar: 50 µm
  • W23166E_PURE_DNER_IHC-P_060925
    IHC staining of DNER was performed on formalin-fixed paraffin-embedded human cerebellum tissue following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704). The tissue sections were incubated with Alexa Fluor® 647 secondary antibody only (panel A) or with anti-DNER (clone W23166E) (panel B). Nuclei were counterstained with DAPI (Cat. No. 422801) (overlay in panel B). Scale Bar: 50 µm
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654651 25 µg 136€
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654652 100 µg 364€
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Description

DNER is a type I transmembrane protein primarily expressed in the nervous system. First identified as a non-canonical Notch ligand, DNER plays a crucial role in neuronal differentiation. DNER is particularly involved in neuron-glial interactions, and abnormalities in DNER function have been associated with neurological disorders and impaired brain development. DNER is expressed in many tissue types and plays a role in a variety of diseases such as COPD, periodontitis, and breast cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein corresponding to the N terminus of human DNER
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

This product is not recommended for use in Western blot (WB), flow cytometry (FC), intracellular flow cytometry (ICFC) or immunocytochemistry (ICC).

RRID
AB_3699080 (BioLegend Cat. No. 654651)
AB_3699080 (BioLegend Cat. No. 654652)

Antigen Details

Structure
DNER is a 737 amino acid protein with an expected molecular weight of 78.5 kD.
Distribution

Cell membrane

Function
Notch activator
Interaction
α- and β- spectrin, GFAP
Ligand/Receptor
Notch 1, Notch 2
Cell Type
Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Antigen References
  1. Eiraku M, et al. 2005. Nat Neurosci. 8:873-80. 
  2. Saito SY and Takeshima H 2006. Cerebellum. 5:227-31. 
  3. Hiseh FY, et al. 2013. Dev Biol. 375:1-12.
Gene ID
92737 View all products for this Gene ID
UniProt
View information about DNER on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.09.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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