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- Clone
- W22042B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Signal Transducer and Activator of Transcription 4, SLEB11, DPMC
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-STAT4 (clone W22042B). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 405405) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker -
IHC staining with Purified anti-STAT4 (clone W22042B) was performed on formalin-fixed paraffin embedded human testis tissue following antigen retrieval using Tris-EDTA pH9.0 Antigen Retrieval Buffer (Cat. No. 422704). The tissue sections were incubated without (panel A) or with Purified anti-STAT4 (clone W22042B) (panels B and C) followed by Alexa Fluor® 647 goat anti-rat (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801) (panels A and C). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 20X objective. Scale bar: 100 µm
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 648848 | 25 µg | 120€ | ||||
| 648849 | 100 µg | 340€ | ||||
Signal Transducer and Activator of Transcription 4 (STAT4) is a critical transcription factor involved in mediating immune responses. STAT4 is primarily activated via phosphorylation which leads to its dimerization, nuclear translocation, and subsequent activation of target genes involved in immune regulation. STAT4 plays a pivotal role in the differentiation of T helper 1 (Th1) cells, follicular helper T cells (Tfh), and regulatory T cells (Tregs), and is essential for the function of natural killer (NK) cells and CD8+ T cells. STAT4 expression is tightly regulated and varies across cell types and activation states, with higher expression observed in activated immune cells such as mature dendritic cells and monocytes. Genetic polymorphisms in the STAT4 gene, such as H623Y and A635V, have been linked to altered STAT4 activity and nuclear localization, influencing susceptibility to various diseases, such as rheumatoid arthritis, systemic lupus erythematosus, type 1 diabetes, and inflammatory bowel disease.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant fragment of human STAT4
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Additional Product Notes
-
For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either of the following:
-Citrate Buffer (Cat. No. 420902)
-Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704)
- RRID
-
AB_3720575 (BioLegend Cat. No. 648848)
AB_3720575 (BioLegend Cat. No. 648849)
Antigen Details
- Structure
- The STAT4 protein is composed of 748 amino acids and has a molecular weight of approximately 86 kDa.
- Distribution
-
STAT4 is primarily located in the cytoplasm of cells in its inactive form. Upon activation, dimerizes and translocates to the nucleus.
- Cell Type
- Dendritic cells, Lymphocytes, Neurons, NK cells, NKT cells, T cells
- Biology Area
- Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology, Neuroscience, Signal Transduction, Transcription Factors
- Molecular Family
- Innate Immune Signaling, Phospho-Proteins
- Antigen References
-
- Xia Y, et al. 2024. Front Immunol. 15:1479418.
- Tolomeo M, et al. 2024. Int Rev Immunol. 43(6):394.
- Yang c, et al. 2020. Int J Biol Sci. 16(9):1575.
- Wurster AL, et al. 2000. Oncogene. 19(21):2577.
- Buzzelli AA, et al. 2023. J Immunol. 210(11):1667.
- Gene ID
- 6775 View all products for this Gene ID
- UniProt
- View information about STAT4 on UniProt.org
Related FAQs
Other Formats
View All STAT4 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-STAT4 | W22042B | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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