Alexa Fluor® 488 anti-Giantin Antibody, Giantin, Poly23A01

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Clone: Poly23A01 (See other available formats)
Regulatory Status: RUO
Other Names: Golgin subfamily B member 1, macrogolgin, golgi integral membrane protein 1, 372 kD Golgi complex-associated protein, golgin B1, golgi integral membrane protein, golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal)
Isotype: Rabbit Polyclonal IgG
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Product Citations: publications

Poly23A01_A488_Giantin_ICC_090123 — HeLa cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Alexa Fluor® 488 anti-Giantin (clone Poly23A01) (panel A). Cells were also co-stained with Alexa Fluor® 647 anti-Tubulinα (clone 10D8) (panel B) and nuclei were counterstained with DAPI (Cat. No. 422801) (panel C). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panel D). Scale bar: 50 µm

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621353

50 µL

120€

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Description

Giantin is a membrane-inserted component of the cis and medial Golgi with a large rod-like cytoplasmic domain. This antibody serves as an excellent marker for the Golgi complex in mammalian cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Recombinant fragment of human giantin N-terminus
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution of 1:200 - 1:800 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Blue Laser (488 nm)
Additional Product Notes: For Immunocytochemistry (ICC), the recommended fixation/permeabilization method is 4% paraformaldehyde/0.5% Triton X-100, or paraformaldehyde followed by ice-cold methanol, or ice-cold methanol fixed only.
RRID: AB_3699063 (BioLegend Cat. No. 621353)

Antigen Details

Structure: Homodimer
Distribution: Widely express in tissues/localized to Golgi apparatus membrane
Function: Participate in forming intercisternal cross-bridges of the Golgi complex
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Golgi Markers
Antigen References: Day PM, et al. 2013. J. Virol. 87:3862-70.

Linstedt AD and Hauri HP. 1993. Mol. Biol. Cell. 4:679-93.
Gene ID: 2804 View all products for this Gene ID
UniProt: View information about Giantin on UniProt.org

Related Products

Description	Clone	Applications

Other Formats

View All Giantin Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Giantin	Poly23A01	ICC
Alexa Fluor® 488 anti-Giantin	Poly23A01	ICC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05.09.2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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