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- Regulatory Status
- RUO
- Other Names
- Immunoglobluin G, IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 432304 | 5 plates | 356€ | ||||
IgG is a monomeric immunoglobulin composed of two identical heavy chains and two identical light chains, linked by inter-chain disulfide bonds. In humans, there are four IgG subclasses—IgG1, IgG2, IgG3, and IgG4—named in order of decreasing abundance. These subclasses differ in the size of their hinge region and in the number and arrangements of the inter-chain disulfide bonds linking their heavy chains. Among the five immunoglobulin isotypes, IgG is the most abundant in human serum. It is synthesized and secreted by plasma B cells and is involved in the secondary immune response. Measuring IgG levels can serve as a diagnostic tool for certain conditions, such as autoimmune diseases, gastrointestinal disorders, and immunoglobulin-deficiency disorders.
BioLegend’s ELISA MAX™ Deluxe Set Human Total IgG contains the components necessary for the accurate quantification of natural and recombinant human IgG. The set is designed for cost-effective and accurate quantification of human IgG in serum, plasma, cerebral spinal fluid, cell culture supernatant, saliva and urine. BioLegend’s ELISA MAX™ Deluxe Sets Human Total IgG are sensitive, accurate, and robust.
Kit Details
- Kit Contents
-
- Human Total IgG ELISA MAX™ Capture Antibody (200X)
- Human Total IgG ELISA MAX™ Detection Antibody (200X)
- Human Total IgG Lyophilized Standard
- Avidin-HRP (1,000X)
- Substrate Solution D
- Coating Buffer A (5X)
- Assay Diluent A (5X)
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 0.69 ng/mL
- Standard Range
- 0.05 - 3 ng/mL
Antigen Details
- Cell Type
- B cells
- Biology Area
- Adaptive Immunity, Immunology
- Gene ID
- NA
- UniProt
- View information about IgG on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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