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ELISpot MAX™ Deluxe Set Human IL-17A

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin 17A, IL-17, CTLA8, cytotoxic T-lymphocyte-associated protein 8
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433924 5 plates 492€
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Description

Interleukin-17A (IL-17A), originally identified as cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) in mice, is a founding member of the IL-17 cytokine family. IL-17A is primarily produced by CD4+ Th17 cells, but also by CD8+ T cells, γδ T cells, and innate lymphoid cells. It signals through a receptor complex of IL-17RA and IL-17RC, activating NF-κB and MAPK pathways, which drive the expression of cytokines, chemokines, and antimicrobial peptides including IL-6, IL-8, G-CSF, GM-CSF, GRO-α, and MCP-1. It plays a key role in the pathogenesis of autoimmune diseases, and IL-17A-targeted therapies are being developed for chronic inflammatory disorders.

BioLegend’s ELISpot MAX™ Deluxe Set includes pre-optimized essential reagents, along with additional buffers and solutions, for conducting an Enzyme-Linked Immunosorbent Spot (ELISpot) assay. This kit is specifically designed to detect human IL-17A-secreting cells. An antibody specific for human IL-17A is coated onto an ethanol-activated 96-well PVDF membrane plate as the capture. Stimulated and/or unstimulated cells are pipetted directly into the wells. The plate is incubated at 37°C for a designated period, during which the capture antibody binds to IL-17A from the secreting cells in the vicinity. After incubation, the cells are removed, and a biotinylated anti-human IL-17A detection antibody is added, followed by avidin-HRP. Next, the TMB membrane substrate is added. The TMB substrate reacts with HRP to form a blue-black precipitate at the sites where IL-17A is present. The result is the appearance of visible spots on the membrane surface, representing the footprints of individual IL-17A-secreting cells.

Product Details
Technical data sheet

Kit Details

Kit Contents
  • Human IL-17A ELISpot MAX™ Capture Antibody (200X) 
  • Human IL-17A ELISpot MAX™ Detection Antibody (200X)
  • Human IL-17A Standard (used as positive control)
  • Avidin-HRP (1000X)
  • TMB Membrane Substrate
  • Coating Buffer A (5X)
  • Assay Diluent B (5X)
Materials Not Included
  • PVDF-bottom plate: Millipore MultiScreenHTS IP Filter Plate (Catalog No. MSIPS4W10) is recommended.
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • 0.22 μm filters
  • 35% Ethanol
  • Sterile PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.22 μm filtered
  • Sterile cell culture media
  • 37°C CO2 incubator
  • Deionized (DI) water
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle, manifold dispenser, or automated microplate washer
  • Tubes for dilution
  • Timer
  • Stereomicroscope or an automated ELISpot reader

Product Details

Verified Reactivity
Human
Application

ELISpot

Antigen Details

Cell Type
T cells
Biology Area
Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokine/Chemokine Receptors
Gene ID
3605 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

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Go To Top Version: 1    Revision Date: 04.21.2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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