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PE anti-mouse CD234 (ACKR1) Antibody

Pricing & Availability
Clone
W22145A (See other available formats)
Regulatory Status
RUO
Other Names
Atypical chemokine receptor 1 (ACKR1), Duffy antigen/chemokine receptor(DARC), FY, Glycoprotein D (GPD)
Isotype
Rat IgG2c, κ
Ave. Rating
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Product Citations
publications
See PE spectral data
Cat # Size Price Quantity Check Availability Save
169003 25 µg 142€
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169004 100 µg 308€
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Description

Atypical chemokine receptor 1 (ACKR1) controls chemokine levels and localization via high-affinity chemokine binding. This chemokine binding doesn’t trigger the classical ligand-driven signal transduction cascades and instead results in chemokine sequestration, degradation, or transcytosis. Ackr1 interacts with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. It acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1 and TARC. It regulates chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms: when expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes; when expressed in erythrocytes, serves as blood reservoir of cognate chemokines but also as a chemokine sink, buffering potential surges in plasma chemokine levels. Ackr1 acts as a receptor for the malaria parasite Plasmodium yoelii in mature erythrocytes. N-terminal glycosylated extracellular domain of ACKR1 carries the Duffy blood group antigens Fya and Fyb.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse full length ACKR1 transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Antigen Details

Structure
Membrane protein of the G-protein coupled receptor 1 family with seven transmembrane domains and four cytoplasmic domains.
Distribution

Expressed mainly on erythrocytes and endothelial cells.

Function
ACKR1 controls chemokine levels, localization, bioavailability and thereby leukocyte recruitment. Acts as a receptor for the malaria parasite Plasmodium yoelii in mature erythrocytes.
Interaction
Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1 and TARC.
Ligand/Receptor
Binds to CXC and CC chemokines subfamilies.
Antigen References
  1. Lou H, et al. 1997. Genome Res. 7:932-41.
  2. Tang T, et al. 1998. DNA Seq. 9:129-143.
  3. Lou H, et al. 2000. Mol Cell Biol. 20:3097-101.
  4. Dawson TC, et al. 2000. Blood. 96:1681-4.
  5. Du J, et al. 2002. J Leukoc Biol. 71:141-53.
  6. Swardson-Olver CJ, et al. 2002. Blood. 99: 2677-84.
  7. Lee JS, et al. 2003. J Immunol. 170:5244-51.
  8. Fukuma N, et al. 2003. Biochem Biophys Res Commun. 303: 137-9.
Gene ID
13349 View all products for this Gene ID
UniProt
View information about CD234 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01.12.2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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