PE anti-Whitlow/218 Linker Antibody, Whitlow/218 Linker, W24150D

Pricing & Availability

Clone: W24150D (See other available formats)
Regulatory Status: RUO
Other Names: Whitlow/218 peptide, Whitlow/218 linker, Whitlow 218 linker
Isotype: Mouse IgG2b, κ
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Product Citations: publications

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900206

25 tests

176€

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900207

100 tests

460€

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Description

The Whitlow/218 linker is an 18 amino acid peptide that is widely used in chimeric antigen receptors (CAR) constructs to connect the variable heavy (VH) and variable light (VL) regions of single-chain variable fragments (scFvs). The linker sequence was designed to increase resistance to proteolytic degradation and minimize aggregation, improving scFv stability and supporting optimal engineered cell function.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clones E3U7Q and W24150D completely block each other.

Antigen Details

Structure: The amino acid sequence GSTSGSGKPGSGEGSTKG, used to connect the variable heavy (VH) and variable light (VL) domains of a single-chain variable fragment (scFv)
Function: Detection of CAR constructs, CAR-T cells
Biology Area: Immunology
Antigen References: Whitlow, M. et al. 1993. Protein Eng. 6:989-95
Gene ID: NA

Documentation

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Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All Whitlow/218 Linker Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 647 anti-Whitlow/218 Linker	W24150D	FC
Purified anti-Whitlow/218 Linker	W24150D	FC
PE anti-Whitlow/218 Linker	W24150D	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 01.07.2026

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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