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Purified anti-COUP-TFI (NR2F1) Antibody

Pricing & Availability
Clone
W23102A (See other available formats)
Regulatory Status
RUO
Other Names
NR2F1, EAR-3, TFCOUP1, COUP transcription factor 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W23102A_PURE_COUP-TF1_WB_072525
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-COUP-TFI (NR2F1) (clone W23102A). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W23102A_PURE_COUP-TF1_WB_072525
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-COUP-TFI (NR2F1) (clone W23102A). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
Cat # Size Price Quantity Check Availability Save
648830 25 µg 140€
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648831 100 µg 360€
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Description

COUP-TFI (Chicken Ovalbumin Upstream Promoter-Transcription Factor I), also known as NR2F1, is a nuclear receptor and transcription factor that plays a pivotal role in neurodevelopment, cell differentiation, and gene regulation. It belongs to the steroid/thyroid hormone receptor superfamily and functions primarily by binding to specific DNA motifs (such as 5'-AGGTCA-3') to regulate gene expression either as a repressor or activator. COUP-TFI is essential for proper cortical development, neuronal migration, and axonal guidance, particularly in the central nervous system. Its expression is tightly regulated during embryogenesis and is critical for establishing regional identity in the developing brain. Dysregulation or mutations in the NR2F1 gene are associated with Bosch-Boonstra-Schaaf optic atrophy syndrome (BBSOAS), a rare neurodevelopmental disorder characterized by intellectual disability, optic nerve atrophy, and developmental delays. Additionally, aberrant COUP-TFI activity has been implicated in various cancers and metabolic disorders.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of Human COUP-TFI protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3720544 (BioLegend Cat. No. 648830)
AB_3720544 (BioLegend Cat. No. 648831)

Antigen Details

Structure
The COUP-TFI protein consists of 423 amino acid and has a molecular weight of approximately 46.2 kD
Distribution

Nuclear

Interaction
CBP, SRC-1, Specific DNA motifs, FABP7
Cell Type
Endothelial cells, Epithelial cells, Monocytes, Neurons, Neutrophils, NK cells, T cells
Biology Area
Cancer Biomarkers, Cell Biology, Neurodegeneration, Neuroscience, Transcription Factors
Antigen References
  1. Wu X, et al. 2022. Neurosci Bull. 38:505.
  2. Feng J, et al. 2021. Sci Adv. 7:eabf6808.
  3. Bonzano S, et al. 2018. Cell Rep. 24:329.
  4. Yang X, et al. 2017. Curr Top Dev Biol. 125:275.
Gene ID
7025 View all products for this Gene ID
UniProt
View information about COUP-TFI on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07.25.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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