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Purified anti-DAP12 (TYROBP) Antibody

Pricing & Availability
Clone
A23012B (See other available formats)
Regulatory Status
RUO
Other Names
TYROBP (TYRO protein tyrosine kinase binding protein), KARAP (Killer Activating Receptor Associated Protein), PLOSL1 (Polycystic Lipomembranous Osteodysplasia with Sclerosing Leukoencephalopathy 1), DNAX-activation protein 12
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. A23012B_PURE_DAP12_WB_061925
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-DAP12 (TYROBP) (clone A23012B). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • A. A23012B_PURE_DAP12_WB_061925
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-DAP12 (TYROBP) (clone A23012B). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • B. A23012B_PURE_DAP12_ICFC_061925
    THP-1 cells (positive cell line, filled histogram) and Jurkat cells (negative cell line, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Purified anti-DAP12 (TYROBP) (clone A23012B), or Purified Mouse IgG1, κ Isotype Control (open histogram, dashed line, representative for both cell lines) (Cat. No. 400101) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322).
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648651 25 µg 136€
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648652 100 µg 344€
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Description

DAP12, also known as TYROBP, is a transmembrane adapter protein expressed in various immune cells, including natural killer cells, monocytes, macrophages, dendritic cells, osteoclasts, and microglia. It plays a role in immune cell functions such as survival, proliferation, phagocytosis, chemotaxis, and cytokine production. DAP12 forms complexes with several immunoreceptors, including TREM2, to trigger intracellular signaling pathways. Regulation of DAP12 involves its interaction with proteins like RER1, which retains unassembled DAP12 in early secretory compartments, preventing its transport to the plasma membrane. Mutations in DAP12 and its associated receptors have been linked to neurodegenerative diseases, such as Nasu-Hakola disease and Alzheimer's disease. Additionally, DAP12 signaling can modulate immune responses, as seen in its paradoxical role in down-regulating cytokine production by plasmacytoid dendritic cells during viral infections.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human DAP12
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using Cyto-Fast™ Fix-Perm Buffer Set (Cat. No. 426803). We do not recommend True-Phos™ Perm Buffer (Cat. No. 425401).

RRID
AB_3717125 (BioLegend Cat. No. 648651)
AB_3717125 (BioLegend Cat. No. 648652)

Antigen Details

Structure
DAP12 is composed of 113 amino acids with a molecular weight approximately 12.2 kDa.
Distribution

Mainly located in the plasma membrane. Also, it can be found in the endoplasmic reticulum and in the ER-golgi intermediate compartment.

Function
• Immune Cell Activation: DAP12 is crucial for activating various immune cells, including natural killer cells, macrophages, and dendritic cells.
• Signal Transduction: It forms complexes with immunoreceptors like TREM2 to trigger intracellular signaling pathways.
• Phagocytosis: Enhances the ability of immune cells to engulf and digest pathogens.
• Cytokine Production: Modulates the production of cytokines, which are essential for immune responses.
• Chemotaxis: Aids in the movement of immune cells towards sites of infection or inflammation.
• Bone Remodeling: In osteoclasts, DAP12 is involved in bone resorption processes.
Interaction
• TREM2: DAP12 forms a complex with TREM2, which is essential for microglial activation and phagocytosis.
• RER1: DAP12 interacts with RER1, which retains unassembled DAP12 in the early secretory compartments, preventing its transport to the plasma membrane.
• NKG2D: This receptor on NK cells and some T cells binds to stress-induced ligands, playing a role in immune responses against tumors and infections.
• SIRP-1β: Another receptor that pairs with DAP12, contributing to immune cell signaling.
• SYK: Spleen tyrosine kinase (SYK) is involved in downstream signaling pathways activated by DAP12.
• PI3K: Phosphoinositide 3-kinase (PI3K) is part of the signaling cascade triggered by DAP12.
Ligand/Receptor
• TREM2: Triggering Receptor Expressed on Myeloid cells 2 (TREM2) binds to DAP12, playing a crucial role in microglial activation and phagocytosis.
• NKG2D: This receptor on natural killer (NK) cells and some T cells binds to stress-induced ligands such as MICA, MICB, and UL16-binding proteins, which are involved in immune responses against tumors and infections.
• SIRP-1β: Signal Regulatory Protein 1β is another receptor that pairs with DAP12, contributing to immune cell signaling
Cell Type
Antigen-presenting cells, Dendritic cells, Macrophages, Monocytes, NK cells, Osteoclasts
Biology Area
Adaptive Immunity, Cell Biology, Immuno-Oncology, Immunology, Innate Immunity, Neuroinflammation, Signal Transduction
Molecular Family
Adaptor Proteins, Innate Immune Signaling
Antigen References
  1. Sjolin H, et al. 2006. J Immunol. 177:2908.
  2. Konishi H, et al. 2018. Front Cell Neurosci. 12:1.
  3. Liu Y, et al. 2024. Cell Mol Life Sci. 81:302.
  4. Liu G, et al. 2023. J Integr Neurosci.22:150.
Regulation
• Protein Interactions: DAP12 interacts with proteins like RER1, which retains unassembled DAP12 in early secretory compartments, preventing its transport to the plasma membrane.
• Gene Expression: The expression of DAP12 can be regulated at the transcriptional level by various cytokines and signaling molecules.
• Post-Translational Modifications: Phosphorylation of DAP12 can modulate its activity and interactions with other proteins.
• Internalization: DAP12 can be internalized into endocytic vesicles, which may regulate its availability and function at the cell surface.
Gene ID
7305 View all products for this Gene ID
UniProt
View information about DAP12 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06.19.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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