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Purified anti-GPX4 Antibody

Pricing & Availability
Clone
W24157D (See other available formats)
Regulatory Status
RUO
Other Names
GPX4, GSHPx-4 (Glutathione peroxidase 4), PHGPx, GPx-4
Isotype
Rat IgG2b, κ
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Product Citations
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649405 25 µg 132€
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649406 100 µg 344€
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Description

Glutathione peroxidase 4 (GPX4) is a selenoenzyme that plays a critical role in protecting cells from oxidative damage by reducing phospholipid hydroperoxides within membranes, thereby preventing lipid peroxidation and ferroptosis. GPX4 is essential for maintaining membrane integrity and redox homeostasis, particularly in tissues rich in polyunsaturated fatty acids like the brain and kidneys. Its expression and activity are tightly regulated through transcriptional, post-transcriptional, epigenetic, and proteostatic mechanisms, and depend on selenium availability and glutathione synthesis. Dysregulation or deficiency of GPX4 is implicated in a range of diseases, including cancer (especially therapy-resistant head and neck cancers), neurodegeneration, infertility, acute kidney injury, and Sedaghatian-type spondylometaphyseal dysplasia.

Product Details
Quality Statement

This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: HAP1 WT Cell Line (Horizon Cat No. Cat. No. C631) and HAP1 GPX4 Knockout cell line (Horizon Cat. No. HZGHC005981c005).

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human GPX4 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

KO/KD-WB, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 2.5 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).

Visit our Technical Protocols Library here to find additional guidance on the relevant applications and experimental conditions.

Antigen Details

Structure
The human GPX4 protein consists of 197 amino acids and has a molecular weight of approximately 22.2 kDa.
Distribution

Cytoplasm, Mitochondria

Interaction
GSR (Glutathione Reductase), PRDX6 (Peroxiredoxin 6), FUNDC1, ALOX15 (Arachidonate 15-lipoxygenase), TXNRD1 (Thioredoxin Reductase 1)
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Mitochondrial Function, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators, Mitochondrial Markers
Antigen References
  1. Lui Y, et al. 2023. Biochem Biophys Acta Rev Cancer. 1878(3):188890
  2. Zhang W, et al. 2024. Biomed Pharmacother. 174:116512
  3. Forcina G, et al. 2019. Proteomics. 19(18):e1800311
Gene ID
2879 View all products for this Gene ID
UniProt
View information about GPX4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.05.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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