- Clone
- Poly9023 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
- Previously
-
Covance Catalog# PRB-101P
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western Blot showing specificity of HA.11, clone Poly9023, at 1:1000 and 1:2000. Lane 1: Molecular weight marker. Lane 2: CHO Clone 6. Lane 3: BSA negative control.
The HA epitope tag is a nine-amino acid peptide sequence (YPYDVPDYA) derived from the human influenza virus hemagglutinin surface glycoprotein (residues 98-106). This well-characterized immunological tag has become an indispensable tool in protein biochemistry and cell biology research due to its exceptional versatility and reliability.
Product DetailsProduct Details
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- This antibody was raised against the twelve amino acid sequence CYPYDVPDYASL.
- Formulation
-
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Previous lots (B472314 and prior) of this product may have been formulated in Phosphate-buffered solution + 0.03% Thimerosal. For further information please contact BioLegend Technical Support or Customer Service. - Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB - Quality tested
IP - Verified
ChIP, ICC, Purification - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 µg/mL (1:1000 dilution). For immunoprecipitation, the suggested use of this reagent is 1:150 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
*BioLegend’s Recombinant Posi-Tag Epitope Tag Protein (Cat. No. 931301) can be used as a helpful positive control for this antibody in western blot.
This antibody recognizes the same hemagglutinin flu epitope YPYDVPDYA as the monoclonal antibody, HA.11 clone 16B12. This polyclonal antibody facilitates epitope tag experiments in murine systems and is useful in experiments involving two proteins labeled with two different tags.
This product may contain other non-IgG subtypes. -
Application References
(PubMed link indicates BioLegend citation) -
- Barrott JJ, et al. 2011. PNAS. 108: 12752. (WB, IP)
- Pecot MY, Malhotra V. 2004. Cell. 116:99.
- Delmas C, et al. 2003. J Biol Chem. 278:12443. (IF)
- Caplan S, et al. 2001. J Cell Biol. 154:109. (IF, WB)
- Yeo C, Whitman M. 2001. Mol Cell 7:949.
- Sander TL, et al. 2000. J Biol Chem.:12857. (WB)
- Bosshart H, et al. 1994. J Cell Biol. 126:1157. (IF, IP)
- Hewawasam G, et al. 2010. Mol Cell. 40:444. (ChIP, WB) PubMed
- Hurov JB, et al. 2004. Curr Biol. 14:736. (IF)
- Shieh AW, et al. 2013. PLoS One. 8:75337. (WB, IF) PubMed
- Hackenbeck T, et al. 2009. Cell Cycle. 8:1386. (ICC)
- Satheshkumar PS, et al. 2013. J Virol. 87:10710.
- Luo R, et al. 2009. Cell Signal. 21:1169. (WB) PubMed
- Berg TJ, et al. 2010. J Biol Chem. 285:35255. (WB) PubMed
- RRID
-
AB_2734670 (BioLegend Cat. No. 902303)
AB_2565018 (BioLegend Cat. No. 902301)
AB_2565019 (BioLegend Cat. No. 902302)
Antigen Details
- Biology Area
- Cell Biology
- Gene ID
- NA
- UniProt
- View information about HA.11 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All HA.11 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Anti-HA Tag | Poly9023 | WB,IP |
| Purified anti-HA.11 Epitope Tag | Poly9023 | WB,IP,ChIP,ICC,Purification |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Anti-HA Tag
Western blot analysis of HA-Tag antibody to Postitag control... -
Purified anti-HA.11 Epitope Tag
Western Blot showing specificity of HA.11, clone Poly9023, a...
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