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Purified anti-Hsp20 Antibody

Pricing & Availability
Clone
W19108A (See other available formats)
Regulatory Status
RUO
Other Names
Heat shock protein beta-6, Heat shock protein family B member 6, Heat shock 20 kDa-like protein p20, HSPB6, Epididymis Luminar Protein 55
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W19108A_PURE_Hsp20_WB_1_072425
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Hsp20 (clone W19108A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W19108A_PURE_Hsp20_WB_1_072425
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Hsp20 (clone W19108A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W19108A_PURE_Hsp20_IHC-P_072425
    IHC staining with Purified anti-Hsp20 (clone W19108A) on formalin-fixed paraffin-embedded colon tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-Hsp20 (clone W19108A) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • C. W19108A_PURE_Hsp20_ICC_072425
    U-87 MG cells (high expressing cell line, panel A) and Hep G2 cells (low expressing cell line, panel B) were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-Hsp20 (clone W19108A), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (panels C and D). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panel C). Scale bar: 50 µm
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648824 25 µg 124€
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648825 100 µg 336€
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Description

Heat shock protein B6 (HSPB6), also known as Hsp20, is a member of the small heat shock protein (sHSP) family. These proteins act as a molecular chaperone by stabilizing partially unfolded proteins, preventing aggregation, and assisting in refolding under stress conditions such as oxidative stress, ischemia, or heat shock. Hsp20 contributes to the maintenance of muscle relaxation and contractility. It interacts with cytoskeletal proteins like actin and myosin, and its phosphorylation, particularly at Ser16 by protein kinase A (PKA) or protein kinase G (PKG), enhances vasorelaxation by modulating actin dynamics and promoting smooth muscle relaxation. The phosphorylation of Hsp20 also confers protection against ischemia-reperfusion injury in cardiac tissue. Additionally, Hsp20 can inhibit apoptosis by interacting with pro-apoptotic proteins such as Bax and preventing mitochondrial membrane permeabilization, thereby maintaining mitochondrial integrity and cell survival. In cardiovascular disease, Hsp20 plays a cardioprotective role. Reduced expression or impaired phosphorylation of Hsp20 has been observed in failing hearts, correlating with increased susceptibility to myocardial infarction and heart failure. Overexpression of Hsp20 in animal models reduces infarct size and improves post-ischemic recovery, emphasizing its potential as a therapeutic target in ischemic heart disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human Hsp20 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

RRID
AB_3717209 (BioLegend Cat. No. 648824)
AB_3717209 (BioLegend Cat. No. 648825)

Antigen Details

Distribution

Cytoplasm and nucleus

Function
Molecular chaperone maintaining denatured proteins in a folding-competent state
Interaction
Forms homodimer; interacts with HSPB1 and HSPB5
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Motility/Cytoskeleton/Structure
Molecular Family
Heat Shock Proteins
Antigen References
  1. Fuchs M, et al. 2009. Biochem. J. 425:245-55.
  2. Dreiza CM, et al. 2010. Cell Stress Chaperones. 15:1-11. 
  3. Weeks SD, et al. 2014. J. Struct. Biol. 185:342-54.
  4. Gardner GT, et al. 2019. JACC Basic Transl. Sci. 4:188-199.
Gene ID
126393 View all products for this Gene ID
UniProt
View information about Hsp20 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07.24.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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