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Purified anti-human CD43 Antibody

Pricing & Availability
Clone
MT1 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. MT1_PURE_CD43_FC_032825
Human peripheral blood lymphocytes were stained with anti-human CD3 (clone OKT-3) APC and purified anti-human CD43 (clone MT1) (left) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG1, κ PE.
  • A. MT1_PURE_CD43_FC_032825
    Human peripheral blood lymphocytes were stained with anti-human CD3 (clone OKT-3) APC and purified anti-human CD43 (clone MT1) (left) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG1, κ PE.
  • B. MT1_PURE_CD43_IHC-P_032825
    Following antigen retrieval using 1X Tris-EDTA Buffer (Cat. No. 422704), formalin-fixed, deparaffinized human spleen tissue sections were incubated with 5 µg/mL of purified anti-human IgD (Cat. No. 324502) and purified mouse IgG1, κ isotype control (Cat. No. 400102) (panel A) or purified anti-human CD43 (clone MT1) (panel B) overnight at 4°C. The tissue was then stained with goat anti-mouse IgG Alexa Fluor® 647 (red) (Cat. No. 405322) and goat anti-rat IgG Alexa Fluor® 594 (green) (Cat. No. 405422). Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Images were captured using a 10X objective. Scale bar: 50 µm
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314311 100 µg 90€
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Description

CD43, also known as Ly-48, leukosialin, sialophorin, leukocyte sialoglycoprotein, and W3/13, is a large single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Stross WP, et al. 1989. J Clin Pathol. 42:953-61.
RRID
AB_3698971 (BioLegend Cat. No. 314311)

Antigen Details

Structure
Single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130 kD
Distribution

Most hematopoietic cells, including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells

Function
Plays dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, inducing apoptosis of T cells and hematopoietic progenitors
Ligand/Receptor
CD54, Siglec-1
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238
  2. Killeen A, et al. 1987. EMBO J. 6:4029-34.
  3. Thurman EC, et al. 1998. Intl. Immunol. 10:691-701.
  4. Onami TM, et al. 2002. J. Immunol. 168:6022-31.
  5. Tong J, et al. 2004. J. Exp. Med. 199:1277-83
  6. Jones AT, et al. 1994. J. Immunol. 153:3426-39.
Gene ID
6693 View all products for this Gene ID
UniProt
View information about CD43 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03.28.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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