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Purified anti-human MMP-8 Antibody

Pricing & Availability
Clone
Poly23A11 (See other available formats)
Regulatory Status
RUO
Other Names
Matrix metalloproteinase (MMP-8), PMNL collagenase (PMNL-CL), Neutrophil Collagenase
Isotype
Goat Polyclonal IgG
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Product Citations
publications
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612160 100 µg 292€
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Description

MMP-8, also termed neutrophil collagenase, is a member of the matrix metalloproteinase family proteins (MMPs). Members of this family are structurally related, zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins. MMP-8 consists of a prodomain that is cleaved upon activation, a catalytic domain containing the zinc binding site, a hinge region, and a carboxyl terminal domain with hemopexin-like repeats. Substrates of MMP-8 include type I, II, and III triple-helical collagens, gelatin peptides, proteoglycans, fibronectin, aggrecan, substance P, serpins, β-casein, and angiotensin. MMP-8 is released by neutrophils upon IL-1β IL-8, GM-CSF and TNF-α stimulation. Besides playing a role in phagocytosis, MMP-8 has been implicated in rheumatoid arthritis (RA) because of its high efficiency in infiltrating connective tissue and breaking down ECM. In fact, serum level of MMP-8 is a strong predictor of mortality in RA, as this is consistent with increased activation of neutrophils in RA. In addition, MMP-8 has been shown to play a crucial role in cardiovascular diseases by promoting atherosclerotic lesion formation. This is supported by the finding that MMP-8 knockout mice have less endothelial cells or plaque angiogenesis in the atherosclerotic plaques. In a clinical study, it was shown that the ratio of MMP-9/TIMP-1, as well as MMP-8 serum levels, could be a useful predictor of prognosis in patients with hepatocellular carcinomas.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant Human MMP-8 (carrier-free)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone Poly23A11) could function as a capture and detection antibody as a self-pairing antibody and using Recombinant Human MMP-8 (carrier-free) protein (Cat. Nos. 556102 and 556104) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.

RRID
AB_3720587 (BioLegend Cat. No. 612160)

Antigen Details

Structure
Monomer
Distribution

Neutrophils, macrophages, monocytes

Function
MMP8 degrades ECM proteins (collagen type I, II, and III, gelatin, proteoglycans, fibronectin, aggrecan, substance P, serpins, β-casein, and angiotensin); IL-1β and IL-6 stimulation activates neutrophils to release MMP-8.
Cell Type
Macrophages, Monocytes, Neutrophils
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References
  1. Nagase H, and Woessner JF Jr. 1999. J. Biol. Chem. 274:21491.
  2. Gill SE and Parks WC. 2008. Int. J. Biochem. Cell Biol. 40:1334.
  3. Mattey DL, et al. 2012. Arthritis Res. Ther. 14:R204.
  4. Fang C. et al. 2013. Cardiovasc. res. 99:146.
  5. Lempinen M. et al. 2013. Ann. Med. 45:482.
  6. Sommer K, et al. 2013. PLos One. 8:e73992.
Gene ID
4317 View all products for this Gene ID
UniProt
View information about MMP-8 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.24.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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