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Purified anti-SIX3 Antibody

Pricing & Availability
Clone
W23154C (See other available formats)
Regulatory Status
RUO
Other Names
Homeobox protein SIX3, HPE2, Sine oculis homebox-like protein 3, Sine oculis homeobox homolog 3
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W23154C_PURE_SIX3_WB_050625
Whole cell lysate from the indicated cell lysates (15 µg total protein) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-SIX3 antibody (clone W23154C). Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W23154C_PURE_SIX3_WB_050625
    Whole cell lysate from the indicated cell lysates (15 µg total protein) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-SIX3 antibody (clone W23154C). Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W23154C_PURE_SIX3_ICC_050625
    Jurkat cells (negative cell line, panel A) and WERI-Rb-1 cells (positive cell line, panel B) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized with 0.5% Triton X-100, and then blocked with 5% FBS. Cells were then stained with Purified anti-SIX3 (clone W23154C) followed by Alexa Fluor® 647 Goat anti-rat IgG antibody (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • C. W23154C_PURE_SIX3_ICFC_050625
    WERI-Rb-1 cells (positive control, filled histogram) and Jurkat cells (negative control, open histogram) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-SIX3 (clone W23154C) or Rat IgG2a, κ Isotype Control (Cat. No. 400502) (open histogram, dashed line), followed by Alexa Fluor® 647 Goat anti-rat IgG antibody (Cat. No. 405416).
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674151 25 µg 132€
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674152 100 µg 360€
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Description

The SIX3 protein is a transcription factor that plays a crucial role in the development of the forebrain and eyes. It functions by binding to specific DNA sequences, regulating the expression of target genes involved in these developmental processes through both repression and activation. During human forebrain development, SIX3 represses WNT1 expression to ensure proper patterning. Mutations in the SIX3 gene are associated with holoprosencephaly type 2 (HPE2), a severe congenital brain malformation characterized by incomplete separation of the forebrain. Additionally, SIX3 mutations have been linked to schizencephaly, a rare brain disorder that results in abnormal clefts or slits in the cerebral hemispheres.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of Human SIX3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using either True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401).

For use of this antibody in Immunocytochemistry (ICC), we recommend fixation/permeabilization using PFA-Methanol or PFA-Triton X-100. We do not recommend Methanol only fixation/permeabilization

RRID
AB_3699092 (BioLegend Cat. No. 674151)
AB_3699092 (BioLegend Cat. No. 674152)

Antigen Details

Structure
The SIX3 protein consists of 332 amino acids and has a molecular weight of approximately 35 kDa.
Distribution

Nuclear 

Function
• Acts as a transcription factor, binding to specific DNA sequences to regulate gene expression.
• Plays a critical role in the development of the forebrain and eyes.
• Involved in the formation of the optic vesicles and the anterior-posterior patterning of the diencephalon.
• Essential for proper lens development and the formation of the proximodistal axis of the optic cup.
Interaction
EYA4, MTA1, TLE1, TLE5
Cell Type
Neural Stem Cells, Neurons
Biology Area
Cell Biology, Neuroscience, Stem Cells, Transcription Factors
Antigen References
  1. Ferrena A, et al. 2024. PLOS One. 19:e0308839
  2. Song X, et al. 2021. Neurosci Bull. 37:985
  3. Yu Z, et al. 2017. J Hematol Oncol. 10:115
  4. Geng X, et al. 2016. Development. 143:4462

 

Gene ID
6496 View all products for this Gene ID
UniProt
View information about SIX3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.06.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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