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Purified anti-SMAD5 Antibody

Pricing & Availability
Clone
W22062B (See other available formats)
Regulatory Status
RUO
Other Names
Mothers against DPP homolog 5, MAD Homolog 5, Mothers againstDPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, hSmad5, MADH5
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
648711 25 µg 140€
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648712 100 µg 360€
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Description

SMAD5 (mothers against decapentaplegic homolog 5) is a receptor-regulated SMAD protein primarily involved in the bone morphogenetic protein (BMP) signaling pathway, which is part of the broader TGF-β superfamily. Upon BMP receptor activation, SMAD5 is phosphorylated and forms a complex with SMAD4, translocating to the nucleus to regulate gene transcription related to cell differentiation, proliferation, apoptosis, and tissue homeostasis. Beyond its canonical role, non-phosphorylated SMAD5 also influences energy metabolism by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Its activity is tightly regulated through phosphorylation, nuclear-cytoplasmic shuttling, and interactions with other proteins such as SMURF1, which mediates its degradation. Dysregulation of SMAD5 has been implicated in several diseases, particularly respiratory disorders such as COPD, asthma, pulmonary arterial hypertension, idiopathic pulmonary fibrosis, and lung cancer.

Product Details
Quality Statement

This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: HAP1 WT Cell Line (Horizon Cat No. C631) and HAP1 SMAD5 Knockout cell line (Horizon Cat. No. HD-HAP1-65253)

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human SMAD5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC, KO/KD WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
The human SMAD5 protein consists of 465 amino acids and has a molecular weight of approximately 52.3 KDa.
Distribution

Located in the cytoplasm and nucleus

Interaction
SNAD4, BMP, SMURF1, CBP/p300, SMAD1, and SMAD8
Biology Area
Angiogenesis, Cancer Biomarkers, Cell Biology, Signal Transduction, Stem Cells, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Lin A, et al. 2024. Mol Med. 30:225
  2. Cao D, et al. 2025. Clin Sci. 139:15.
  3. Orlowski J. 2017. Cell Res. 27:1075.
Gene ID
4090 View all products for this Gene ID
UniProt
View information about SMAD5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01.21.2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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