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- Clone
- 6E9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Triggering receptor expressed on myeloid cells 2, Trem, TREM-2, Trem2a, Trem2b, Trem2c, TREM2
- Previously
-
Covance Catalog# MRT-5093
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
J774 cells (mouse macrophage cell line) were stained with purified Trem-2 (clone 6E9, filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG PE secondary antibody.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 824801 | 1 mL | 266€ | ||||
TREM-2 is a 26 kD transmembrane glycoprotein that consists of a single extracellular immunoglobulin-like domain, a transmembrane region with a charged lysine residue and a short cytoplasmic tail. It associates with DNAX-activation protein 12 (DAP12) for signaling and function. TREM-2 is expressed on immature monocyte-derived dendritic cells. After activation by microbial products or tumor necrosis factor (TNF) and TNF-related proteins, dendritic cells downregulate the expression of TREM-2. TREM-2 is also expressed by osteoclasts and microglia, where it is involved in bone modeling and brain function, respectively. Another role of TREM-2 might be promoting the removal of apoptotic cells, organic matrix and macromolecules by microglia.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- This monoclonal antibody was raised against a mouse recombinant TREM-2 protein.
- Formulation
- Phosphate-buffered solution + 0.1% BSA and 0.02% NaN3.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.1 mg/ml
- Storage & Handling
- Store between 2°C and 8°C.
- Application
-
FC, IP
- Recommended Usage
-
Working dilutions depend upon detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting dilution for immunoprecipitation and flow cytometry is 1:50.
- Application Notes
-
This antibody is effective in immunoprecipitation (IP) and flow cytometry (FC).
Monoclonal antibody 6E9 recognizes mouse membrane-bound as well as soluble triggering receptor expressed on myeloid cells-2 (TREM-2). -
Application References
(PubMed link indicates BioLegend citation) -
- Koga, T et al. Costimulatory signals mediated by the ITAM motif cooperate with RANKL for bone homeostasis. Nature 428(6984): 758-763, 2004.
- RRID
-
AB_2564884 (BioLegend Cat. No. 824801)
Antigen Details
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience
- Gene ID
- 83433 View all products for this Gene ID
- UniProt
- View information about TREM2 on UniProt.org
Related FAQs
Other Formats
View All TREM-2 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-TREM-2 | 6E9 | FC, IP |
| Purified anti-mouse TREM2 | 6E9 | FC,IP |
| PE anti-mouse TREM2 | 6E9 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-TREM-2
J774 cells (mouse macrophage cell line) were stained with pu... -
Purified anti-mouse TREM2
Thioglicollate-induced macrophages from the perithoneal cavi... -
PE anti-mouse TREM2
Thioglicollate-induced macrophages from the peritoneal cavit...
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