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Purified anti-UCHL1 Antibody

Pricing & Availability
Clone
A19092E (See other available formats)
Regulatory Status
RUO
Other Names
Ubiquitin carboxyl-terminal hydrolase 1, PGP9.5, Ubiquitin Carboxyl-Terminal Esterase L1, Neuron Cytoplasmic Protein 9.5, HEL-117, UCH-L1, HEL-S-53, NDGOA, PARK5, PGP95, SPG79
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
a-A19092E_PURE_UCHL1_Antibody_120821
Whole cell extracts (15 µg total protein) from Ramos and A549 (positive control) lysates, K-562 and HeLa (negative control) lysates, and mouse brain lysates were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1:8000 dilution of purified anti-UCHL1 antibody (clone A19092E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker
  • a-A19092E_PURE_UCHL1_Antibody_120821
    Whole cell extracts (15 µg total protein) from Ramos and A549 (positive control) lysates, K-562 and HeLa (negative control) lysates, and mouse brain lysates were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1:8000 dilution of purified anti-UCHL1 antibody (clone A19092E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker
  • b-A19092E_PURE_UCHL1_Antibody_092523
    IHC staining of Purified anti-UCHL1 (clone A19092E) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902), the tissue was incubated with (panel A) and without (panel B) 5 µg/mL of Purified anti-UCHL1 (clone A19092E) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X (panel A and B), 20X (panel C) and 40X (panel D) objective. Scale bar: 50 µm
  • c-A19092E_PURE_UCHL1_Antibody_3_081225
    Whole cell extracts (15 µg total protein per lane) from HAP1 and HAP1 UCHL1 KO cells (Horizon Cat. No. HZGHC003960c004) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-UCHL1 (clone A19092E). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • d-A19092E_PURE_UCHL1_Antibody_4_081225
    A549 (panel A), HAP1 (panel B), and HAP1 UCHL1 KO cells (panel C, Horizon Cat. No. HZGHC003960c004) were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-UCHL1 (clone A19092E), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panels D, E, and F). Scale bar: 50 µm
  • e-A19092E_PURE_UCHL1_Antibody_5_081225
    HAP1 cells (positive cell line, filled histogram) and HAP1 UCHL1 KO cells (negative cell line, open histogram, Horizon Cat. No. HZGHC003960c004) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Purified anti-UCHL1 (A19092E), or Purified Mouse IgG2b, κ isotype Ctrl (dashed histogram, representative of both the positive and negative cell lines) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322).
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601352 100 µL 0€
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601351 25 µL 113€
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Description

Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) is a thiol protease and member of the ubiquitin carboxyl-terminal hydrolase (UHC) family of deubiquitinating enzymes (DUBs). Highly expressed in the brain and neuroendocrine system, UCHL1 maintains long-term potentiation (LTP), regulates synaptic structure and function, plays a role in the protection of neuroendocrine cells, and is required for the structure and function of neuromuscular junctions. As an important component of the neuronal ubiquitin-proteasome pathway (UPP), UCHL1 tags aberrant proteins for proteasomal degradation, stabilizes and recycles free ubiquitin, regulates axonal transport, plays a critical role in axonal and neuronal integrity and repair, and may protect them from hypoxia-induced cell death following injury and ischemia. Loss or mutation of UCHL1 has been linked to axonal and dendritic pathology and subsequent deterioration of cognitive and motor functions and neurological disorders such as Alzheimer’s disease, Parkinson’s disease, and Amyotrophic lateral sclerosis, thus making it an important potential therapeutic target for such disorders.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human UCHL1 protein
Formulation
PBS, 0.5% BSA, 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:8000. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10 μg/mL is recommended. For flow cytometric staining using our Cyto-Fast™ Fix/Perm Buffer Set, the suggested use of this reagent is  ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

This clone was tested for ICC using HeLa cells (negative control) and Ramos cells (positive control) using three different fixation/permeabilization methods: methanol only, 4% PFA + methanol, and 4% PFA + Triton X-100. Each method produced nuclear and cytosolic staining in positive controls as well as cytosolic staining in negative controls.

This clone failed to adequately enrich UCHL1 from Ramos whole cell extracts.

For immunohistochemistry (IHC-P), Citrate Buffer, 10X (Cat. No. 420902) as antigen retrieval system is recommended.

RRID
AB_2904442 (BioLegend Cat. No. 601351)

Antigen Details

Structure
UCHL1 is a 223 amino acid protein with an expected molecular weight of 27 kD.
Distribution

Expressed primarily in the central nervous system and gonads / Endoplasmic reticulum and cytoplasm

Function
Hydrolase, Thiol protease
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroscience, Ubiquitin/Protein Degradation
Antigen References
  1. Bilguval K, et al. 2013. PNAS. 110:3489-94.
  2. Bishop P, et al. 2016. Biochem. J. 473:2453-62.
  3. Graham S and Liu H. 2017. Ageing Res. Rev. 34:30-38.
Gene ID
7345 View all products for this Gene ID
UniProt
View information about UCHL1 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All UCHL1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-UCHL1 A19092E WB,IHC-P,ICC,ICFC
Go To Top Version: 4    Revision Date: 02.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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