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Purified anti-XIAP Antibody

Pricing & Availability
Clone
W23268E (See other available formats)
Regulatory Status
RUO
Other Names
E3 ubiquitin-protein ligase XIAP, Baculoviral IAP repeat-containing protein 4,BIRC4, IAP-like protein, ILP, Inhibitor of apoptosis protein 3, IAP3, RING-type E3 ubiquitin transferase XIAP, X-linked inhibitor of apoptosis protein
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
4. W23268E_PURE_XIAP_Antibody_4_012226
HAP1 wild-type cells (positive cell line, top panels) (HAP1 parental cell line, Horizon Cat No. C631) and HAP1 XIAP Knockout cells (negative cell line, bottom panels) (HAP1 XIAP KO cell line , Horizon Cat No. HZGHC008092c010) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized with ice-cold methanol, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-XIAP (clone W23268E), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on Revvity Operetta CLS™ High Content Analysis System with a 40X objective and merged. Scale bar: 50 µm
  • 4. W23268E_PURE_XIAP_Antibody_4_012226
    HAP1 wild-type cells (positive cell line, top panels) (HAP1 parental cell line, Horizon Cat No. C631) and HAP1 XIAP Knockout cells (negative cell line, bottom panels) (HAP1 XIAP KO cell line , Horizon Cat No. HZGHC008092c010) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized with ice-cold methanol, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-XIAP (clone W23268E), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on Revvity Operetta CLS™ High Content Analysis System with a 40X objective and merged. Scale bar: 50 µm
  • 5. W23268E_PURE_XIAP_Antibody_5_012226
    HAP1 wild-type cells (positive cell line, filled histogram) (HAP1 parental cell line, Horizon Cat No. C631) and HAP1 XIAP Knockout cells (negative cell line, open histogram) (Horizon Cat. No. HZGHC008092c010) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with Purified anti-XIAP (clone W23268E) or Purified Rat IgG2a, κ Isotype Control (dashed histogram, representative of both cell lines) (Cat No. 400502), followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416).
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648727 25 µg 128€
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648728 100 µg 344€
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Description

XIAP, also known as BIRC4, is one of the potent endogenous suppressors of apoptosis in humans. It belongs to the inhibitor of apoptosis protein (IAP) family, a group characterized by the presence of one or more baculoviral IAP repeat (BIR) domains that mediate interactions with apoptotic proteins. XIAP serves as a direct inhibitor of key effector caspases, functioning as a crucial regulator of programmed cell death and cellular homeostasis.

XIAP inhibits the proteolytic activity of caspase-3, caspase-7, and caspase-9, the principal executioners of the intrinsic apoptotic pathway. The BIR2 domain and the adjacent linker region inhibit caspase-3 and caspase-7, while the BIR3 domain inhibits caspase-9. Blocking XIAP prevents the downstream cleavage of cellular substrates that culminates in apoptotic disassembly. XIAP also contains a RING finger E3 ubiquitin ligase domain, allowing it to ubiquitinate caspases and other target proteins, which can direct them toward proteasomal degradation.

Overexpression of XIAP is observed in many cancers, including colorectal, pancreatic, breast, and hematological malignancies. Mutations in the XIAP/BIRC4 gene impair its regulatory functions, especially in pathways involving NOD2 signaling and immune homeostasis. Patients with XIAP deficiency exhibit heightened susceptibility to hemophagocytic lymphohistiocytosis (HLH), severe inflammatory responses, chronic colitis resembling Crohn’s disease, and dysregulated immune cell survival. 

Product Details
Quality Statement

This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: Human HAP1 XIAP Knockout Cell Line (Horizon Cat. No. HZGHC008092c010)

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human XIAP
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, ICFC, KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25- 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with either Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).

Antigen Details

Structure
XIAP is a 497 amino acid protein with a predicted molecular weight of 56.7 kD.
Distribution

Cytoplasm and nucleus

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Proliferation and Viability, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
Molecular Family
Enzymes and Regulators
Antigen References
  1. Du C, et al. 2000. Cell. 102:33-42.
  2. Schimmer A D, et al. 2004. Cancer Cell. 5:25-35.
  3. Scott F L, et al. 2005. EMBO J. 24:645–655. 
  4. Cheung CHA, et al. 2020. J. Biomed. Sci. 27:31.
Gene ID
331 View all products for this Gene ID
UniProt
View information about XIAP on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All XIAP Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-XIAP W23268E WB,IHC-P,ICC,ICFC
Go To Top Version: 1    Revision Date: 01.22.2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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