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Spark PLUS UV395™ anti-mouse CD26 (DPP-4) (Flexi-Fluor5™) Antibody

Pricing & Availability
Clone
H194-112 (See other available formats)
Regulatory Status
RUO
Other Names
Dipeptidyl peptidase 4, THAM, DPP4
Isotype
Rat IgG2a, κ
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Product Citations
publications
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Cat # Size Price Quantity Save
285693 50 µg 247€
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Description

CD26, also known as DPP IV or THAM, is a 220 kD type II transmembrane homodimer. It consists of an α/β hydrolase domain and an eight-blade beta-propeller domain.  After proteolysis of the membrane-bound CD26, a soluble form of DPP IV is released. CD26 is expressed on thymocytes (development dependent), T cells, B cells, NK cells, and macrophages. It is involved in T cell costimulation, endothelial cell migration and proteolysis processes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark PLUS UV395™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC
Recommended Usage

Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

* Spark PLUS UV395™ has a maximum excitation of 355 nm and a maximum emission of 385 nm.

Excitation Laser
Ultraviolet Laser (355 nm)
Application Notes

Additional reported applications include: in vitro activation of thymocytes1, immunoprecipitation from cell lysates and cell-free supernatants1,2 and immunohistochemical staining of frozen tissue sections1.

Additional Product Notes

For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Application References

(PubMed link indicates BioLegend citation)
  1. Naquet P, et al. 1988. J. Immunol. 141:4101.
  2. Vivier I, et al. 1991. J. Immunol. 147:447.
  3. Sen A, et al. 2012. PNAS PubMed
  4. Coleman CM, et al. 2014. J Gen Virol. 95:408. PubMed

Antigen Details

Structure
Homodimer, each monomer is a single chain type II transmembranal molecule of 110 kD that consists of two domains; soluble form is released after proteolysis of the membrane form
Distribution

Mature thymocytes, activated T cells, B cells, NK cells, macrophages

Function
T cell costimulation, endothelial cell migration, proteolysis
Interaction
CAV1, IGF2R, PTPRC, BCL10, CARD1, IKBKB
Ligand/Receptor
ADA, collagen
Cell Type
B cells, Macrophages, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cooper KG, et al. 2009. Infect. Immun. 77:2447.
2. Eltzschig HK, et al. 2006. Blood 108:1602.
3. Peranteau WH, et al. 2006. Blood 108:4268.

Gene ID
13482 View all products for this Gene ID
UniProt
View information about CD26 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03.09.2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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