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Spark YG™ 581 anti-p53 Phospho (Ser15) Recombinant Antibody

Pricing & Availability
Clone
P53S15-1C11.Rec (See other available formats)
Regulatory Status
RUO
Other Names
P53, Tumor protein 53, LFS1, Tumor suppressor p53, Cellular tumor antigen P53, Antigen NY-CO-13, Phosphoprotein p53
Isotype
Rabbit IgG
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Product Citations
publications
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640755 25 µg 144€
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640756 100 µg 384€
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Description

The p53 transcription factor is a tumor suppressor which regulates genome stability via the cell cycle, DNA repair, apoptosis, and senescence. It is activated in response to various cellular stresses including DNA damage. Mutations in TP53 gene are found in approximately half of all human cancers, making it one of the most mutated genes in cancer. These mutations often lead to loss of tumor-suppressing functions, contributing to uncontrolled cell proliferation and tumor development. Phosphorylation at Serine 15 is particularly important for p53 activation and function. This modification, often triggered by DNA damage, enhances p53 stability and its ability to activate target genes involved in cell cycle arrest and apoptosis. Without this phosphorylation, p53 ability to respond to stress signals and prevent tumorigenesis is significantly impaired.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Rabbit
Immunogen
Synthetic peptide of human p53 phosphorylated at Ser15
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark YG™ 581 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.015 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 581 has a maximum excitation of 562 nm and a maximum emission of 581 nm.

Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401).

For use of this antibody in immunocytochemistry (ICC), we recommend fixation and permeabilization using either PFA-Triton X-100 or PFA-ice-cold methanol.

Visit our Technical Protocols Library here to find additional guidance on the relevant applications and experimental conditions.

Antigen Details

Structure
The p53 protein consists of 393 amino acids and has a molecular weight of approximately 53 kDa.
Distribution

- Under normal conditions, p53 is present at low levels and shuttles between the nucleus and the cytoplasm.
- Upon activation by stress signals such as DNA damage, p53 accumulates in the nucleus to initiate DNA repair, cell cycle arrest, or apoptosis.
- p53 can localize to the mitochondria to promote apoptosis by interacting with pro- and anti-apoptotic protein.

Function
• Cell Cycle Arrest: p53 can halt the cell cycle to allow for DNA repair before the cell proceeds to divide.
• DNA Repair: It activates genes involved in DNA repair mechanisms, ensuring that damaged DNA is corrected.
• Apoptosis: If the DNA damage is irreparable, p53 can initiate programmed cell death (apoptosis) to prevent the propagation of damaged cells.
• Senescence: p53 can induce a state of permanent cell cycle arrest known as senescence, preventing damaged cells from dividing.
• Metabolic Regulation: It also plays a role in regulating cellular metabolism and oxidative stress responses.
Interaction
MDM2: This protein is a major negative regulator of p53. It binds to p53, leading to its ubiquitination and degradation.
p21: p53 activates the transcription of p21, a cyclin-dependent kinase inhibitor that plays a role in cell cycle arrest.
BAX: p53 induces the expression of BAX, a pro-apoptotic protein that promotes apoptosis.
PUMA and NOXA: These are other pro-apoptotic proteins regulated by p53, involved in the intrinsic pathway of apoptosis.
ATM and ATR: These kinases phosphorylate p53 in response to DNA damage, stabilizing and activating it.
BRCA1: p53 interacts with BRCA1, a protein involved in DNA repair.
53BP1: This protein binds to p53 and is involved in DNA damage response.
CBP/p300: These are transcriptional co-activators that acetylate p53, enhancing its transcriptional activity.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, DNA Repair/Replication, Signal Transduction, Transcription Factors
Molecular Family
Phospho-Proteins, Tumor Suppressors
Antigen References
  1. Loughery J, et al. 2014. Nucleic Acids Research. 42:7666.
  2. Marei HE, et al. 2021. Cancer Cell International. 21:703.
  3. Ahmadi SE, et al. 2024. Biomarker Research. 12:137.
  4. Timofeev O, et al. 2021. Cancers. 13:2422.
Regulation
• Post-Translational Modifications: Phosphorylation, acetylation, and ubiquitination are key modifications that regulate p53 stability and activity.
• MDM2 Interaction: The protein MDM2 is a major negative regulator of p53. It binds to p53, leading to its ubiquitination and subsequent degradation.
• Stress Signals: In response to cellular stress such as DNA damage, hypoxia, or oncogene activation, p53 is stabilized and activated to carry out its tumor suppressor functions.
• Mutations in the TP53 gene, is a common feature in many cancers, leading to uncontrolled cell proliferation and tumor development.
Gene ID
7157 View all products for this Gene ID
UniProt
View information about p53 Phospho Ser15 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 02.09.2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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