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Ultra-LEAF™ Purified anti-human CD28H (TMIGD2) Antibody

Pricing & Availability
Clone
W23119G (See other available formats)
Regulatory Status
RUO
Other Names
TMIGD2, Transmembrane And Immunoglobulin Domain Containing 2, IGPR-1, CD28H
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
648725 100 µg 272€
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648726 1 mg 680€
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Description

CD28 homolog (CD28H), also referred to as TMIGD2 (Transmembrane and Immunoglobulin Domain Containing 2) and IGPR-1 (Immunoglobulin-containing and Proline-rich Receptor-1), is a member of the immunoglobulin superfamily. It is predominantly expressed in epithelial and endothelial cells. The CD28H structure consists of an extracellular domain resembling an immunoglobulin V-like (IgV-like) domain, which contains two potential glycosylation sites. It also features a transmembrane segment and a cytoplasmic tail with a tyrosine residue. CD28H was previously recognized both as a co-stimulatory receptor and as an adhesion molecule. HHLA2 (B7-H7) is the known ligand for CD28H. Connection of the CD28H and HHLA2 primarily co-stimulates human T cell growth through an Akt-dependent signaling cascade which mediate tumor resistance against NK cells and promotes cytokine production. CD28H is associated with immune dysregulation and angiogenesis. In autoimmune diseases, abnormal CD28H signaling can cause inappropriate T cell activation, resulting in tissue damage. Additionally, CD28H expression can promote the abnormal self-renewal and differentiation of acute myeloid leukemia cells via downstream signaling pathways like ERK1/2-p90RSK-CREB signaling. Targeting CD28 signaling with anti-CD28 mAb attenuates self-renewal of leukemia stem cells, providing promising therapeutic value.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human CD28H (TMIGD2)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 400 ng/mL Biotinylated Recombinant Human CD28H-Fc Chimera to 1 μg/mL immobilized Recombinant Human B7-H7. ND50 range: 0.08 – 0.48 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Dimer
Distribution

NK cell, T cell, epithelial and endothelial cells

Function
Regulates lymphocyte responses, boosts T-cell proliferation, and plays a crucial role in cell migration and the formation of new blood vessels.
Interaction
B7-H7/HHLA2
Ligand/Receptor
B7 family
Cell Type
Epithelial cells, NK cells, T cells
Biology Area
Angiogenesis, Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology
Antigen References
  1. Burke, Kelly P et al. 2024. Immunity. 57(2): 223-244.
  2. Zhu Yuwen, et al. 2013. Nat Commun. 4:2043.
  3. Kula Agnieszka, et al. 2024. Cells. 13:794
  4. Wang Hao, et al. 2024. Nature communications. 15.11. 
  5. Janakiram Murali, et al. 2017. Immunol Rev. 276:26-39.
  6. Nagai Shigenori and Miyuki Azuma. 2019. Advances in experimental medicine and biology. 1189: 25-51.
  7. Zhong C, et al. 2020. Clinical and experimental immunology. 200:12-21
  8. Tian Yu, et al. 2018. Oncoimmunology. 8:e1538440
  9. Ying Honggang, et al. 2022. EBioMedicine. 79:103987.
  10. Amraei Razie, et al. 2020. J Biol Chem. 295:16691-1669.
Gene ID
126259 View all products for this Gene ID
UniProt
View information about CD28H on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 01.27.2026

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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