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Ultra-LEAF™ Purified anti-human PDGFRα Antibody

Pricing & Availability
Clone
W22075L (See other available formats)
Regulatory Status
RUO
Other Names
Platelet Derived Growth Factor Receptor Alpha, PDGFR2, CD140a, CD140 Antigen-Like Family Member A
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W22075L_ULEAF_PDGFRa_Block_063025
Recombinant Human PDGFRα-Fc Chimera (carrier-free) (Cat. No. 569906) (black circles) inhibits the proliferation of NIH3T3 mouse embryonic fibroblast cells in the presence of Recombinant Human PDGF-AA (carrier-free) (Cat. No. 773706). Ultra-LEAF™ purified anti-human PDGFRα (clone W22075L) (purple squares) inhibits the effect of Recombinant Human PDGFRα-Fc Chimera (6 µg/mL) in a dose-dependent manner, whereas the Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Control Antibody (Cat. No. 400644) (gray triangles) does not have the effect. ND50 range: 1.5 – 10 µg/mL.
  • W22075L_ULEAF_PDGFRa_Block_063025
    Recombinant Human PDGFRα-Fc Chimera (carrier-free) (Cat. No. 569906) (black circles) inhibits the proliferation of NIH3T3 mouse embryonic fibroblast cells in the presence of Recombinant Human PDGF-AA (carrier-free) (Cat. No. 773706). Ultra-LEAF™ purified anti-human PDGFRα (clone W22075L) (purple squares) inhibits the effect of Recombinant Human PDGFRα-Fc Chimera (6 µg/mL) in a dose-dependent manner, whereas the Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Control Antibody (Cat. No. 400644) (gray triangles) does not have the effect. ND50 range: 1.5 – 10 µg/mL.
Cat # Size Price Quantity Check Availability Save
648803 100 µg 256€
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648804 1 mg 644€
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Description

The Platelet-derived growth factor (PDGF) family comprises of four different polypeptide chains encoded by four genes. The four PDGF chains assemble into disulfide-bonded dimers via homo- or heterodimerization. Five different dimeric isoforms have been described so far; PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC, and PDGF-DD. PDGF is a major mitogen for fibroblasts, smooth muscle cells, and others. They exert their functions by interacting with two related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). Upon ligand binding, PDGFRα undergoes dimerization and autophosphorylation, resulting in the formation of docking sites for SH2 domain-containing signaling proteins. These recruited molecules include enzymatic effectors (e.g., Src family kinases, phosphatidylinositol 3-kinase, Phospholipase Cy1, rasGAP, tyrosine phosphatase Syp) and adaptor proteins (e.g., GRB-2, Shc, Nck). The SH2 domains of these proteins specifically recognize phosphorylated PDGFRα, mediating the activation of downstream signaling pathways. PDGFRα plays a key role in many diseases through the above signal transduction.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human PDGFRα
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the effect of Recombinant Human PDGFRα-Fc Chimera (6 μg/mL) in NIH3T3 cells in a dose dependent manner. ND50 range: 1.5 – 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each
application.

RRID
AB_3720509 (BioLegend Cat. No. 648803)
AB_3720509 (BioLegend Cat. No. 648804)

Antigen Details

Structure
Homodimer
Distribution

Present in a variety of mesenchymal tissues such as the lungs, heart, intestines, and skin.

Function
The binding of its cognate ligands results in the activation of multiple signaling cascades such as MAPK, STAT, and PI3K pathways resulting in stimulation of cell proliferation, regulation of cell migration, and chemotaxis in wound healing.
Interaction
Mesenchymal cells, Oligodendrocyte
Ligand/Receptor
Human PDGFAA, PDGFAB, PDGFBB, PDGFCC and PDGFDD
Cell Type
Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Claesson-Welsh L. 1994. Prog Growth Factor Res. 5:37-54.
  2. Boström H, et al. 1996. Cell. 85:863-73.
  3. Heldin CH, et al. 1999. Physiol Rev. 79:1283-316.
  4. Fredriksson L, et al. 2004. Cytokine Growth Factor Rev. 15:197-204.
  5. Andrae J, et al. 2008. Genes Dev. 22:1276-312.
  6. Yao L, et al. 2022. Cell Rep. 40:111192. 
  7. Vantler M, et al. 2006. FEBS Lett. 580:6769-76.
  8. Vassbotn FS, et al. 1994. J Biol Chem.269:13874-9.
  9. Li R, et al. 2018. Elife.7:e36865.
Gene ID
5156 View all products for this Gene ID
UniProt
View information about PDGFRalpha on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06.30.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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