CD3/CD28-stimulated human PBMCs (3 days) were treated with 10 mM 2-Deoxy-D-Glucose (2-DG), 1 µM Oligomycin A, a combination of both, or vehicle (Control) for 15 minutes at 37°C. Cells were then stained with ATP Red (Cat. No. 421944) and the ATP Red fluorescence intensity of CD3+ T cells was analyzed by flow cytometry. Error bars represent standard deviation from 3 different donors.
CD3/CD28-stimulated human PBMCs (3 days) were treated with 10 mM 2-Deoxy-D-Glucose (2-DG), 1 µM Oligomycin A, a combination of both, or vehicle (Control) for 15 minutes at 37°C. Cells were then stained with ATP Red (Cat. No. 421944) and the ATP Red fluorescence intensity of CD3+ T cells was analyzed by flow cytometry. Error bars represent standard deviation from 3 different donors.
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427505
250 mg
DKK238
Description
2-Deoxy-D-glucose (2-DG) blocks glycolysis by inhibiting glucose-6-phosphate production. 2-DG is transported into the cell by glucose transporters where it is phosphorylated by hexokinase. Phosphorylated 2-DG cannot be further metabolized by phosphoglucose isomerase and accumulates in the cell, leading to the depletion of cellular ATP. 2-DG is used in metabolic studies to determine cellular dependency on glycolysis.
Resuspend 1 g of lyophilized 2-Deoxy-D-Glucose in 60.9 mL of water or PBS to make a 100 mM stock solution. For 250 mg size, add 15.2 mL water or PBS to make a 100 mM stock solution.
Storage & Handling
Store lyophilized powder at -20°C. Once reconstituted, store aliquoted at -80°C. Use reconstituted material within 6 months to avoid loss of potency. Avoid freeze thaw cycles.
2-Deoxy-D-glucose can be used for inhibition of glycolysis. For primary immune cells (e.g. PBMCs or splenocytes) a range of 2.5 – 10.0 mM is recommended. It is recommended that the reagent be titrated for each experiment to determine optimal performance.
CD3/CD28-stimulated human PBMCs (3 days) were treated with 10 mM 2-Deoxy-D-Glucose (2-DG), 1 µM Oligomycin A, a combination of both, or vehicle (Control) for 15 minutes at 37°C. Cells were then stained with ATP Red (Cat. No. 421944) and the ATP Red fluorescence intensity of CD3+ T cells was analyzed by flow cytometry. Error bars represent standard deviation from 3 different donors.
CD3/CD28-stimulated human PBMCs (3 days) were treated with 10 mM 2-Deoxy-D-Glucose (2-DG), 1 µM Oligomycin A, a combination of both, or vehicle (Control) for 15 minutes at 37°C. Cells were then stained with ATP Red (Cat. No. 421944) and the ATP Red fluorescence intensity of CD3+ T cells was analyzed by flow cytometry. Error bars represent standard deviation from 3 different donors.
Cat #
Size
Price
Quantity
Check Availability
Save
Input string was not in a correct format.
427505
250 mg
DKK238
Description
2-Deoxy-D-glucose (2-DG) blocks glycolysis by inhibiting glucose-6-phosphate production. 2-DG is transported into the cell by glucose transporters where it is phosphorylated by hexokinase. Phosphorylated 2-DG cannot be further metabolized by phosphoglucose isomerase and accumulates in the cell, leading to the depletion of cellular ATP. 2-DG is used in metabolic studies to determine cellular dependency on glycolysis.
Resuspend 1 g of lyophilized 2-Deoxy-D-Glucose in 60.9 mL of water or PBS to make a 100 mM stock solution. For 250 mg size, add 15.2 mL water or PBS to make a 100 mM stock solution.
Storage & Handling
Store lyophilized powder at -20°C. Once reconstituted, store aliquoted at -80°C. Use reconstituted material within 6 months to avoid loss of potency. Avoid freeze thaw cycles.
2-Deoxy-D-glucose can be used for inhibition of glycolysis. For primary immune cells (e.g. PBMCs or splenocytes) a range of 2.5 – 10.0 mM is recommended. It is recommended that the reagent be titrated for each experiment to determine optimal performance.
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