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APC anti-mouse IL-17A Antibody APC anti-mouse IL-17A Antibody

Pricing & Availability
Clone
TC11-18H10.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
TC11-18H10_APC_031808
PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 APC
  • TC11-18H10_APC_031808
    PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
506915 25 µg DKK779
Check Availability


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Request Bulk Quote
506916 100 µg DKK1759
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Description

IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The IL-17A antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References
  1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
  2. Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
  3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
  4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
  5. Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
  6. Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
  7. Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
  8. Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
  9. Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
  10. Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
  11. Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
  12. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
  13. Kivisäkk P, et al. 2009. Ann. Neurol. 65:457. PubMed
  14. Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
  15. Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
  16. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Miki H, et al. 2020. J Immunol. 204:1892. PubMed
  2. Zutshi S, et al. 2021. Immunology. 163:460. PubMed
  3. Yang L, et al. 2022. Acta Pharmacol Sin. 43:963. PubMed
  4. Wang YC, et al. 2023. J Exp Med. 220:. PubMed
  5. Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed
  6. Lebson L, et al. 2010. J Immunol. 185:7161. PubMed
  7. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  8. Su Y, et al. 2022. J Hematol Oncol. 15:99. PubMed
  9. Liu J, et al. 2019. Immunity. 50:600. PubMed
  10. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  11. Gerber AN, et al. 2021. Cell Rep. 37:109816. PubMed
  12. Manivasagam S, et al. 2022. J Immunol. 208:1341. PubMed
  13. Kenichi Shimada et al. 2018. Immunity. 49(5):873-885 . PubMed
  14. MaruYama T 2015. Biochem Biophys Res Commun. 464: 586-589. PubMed
  15. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  16. Andreas N, et al. 2021. Arthritis Res Ther. 23:222. PubMed
  17. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  18. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  19. Li J, et al. 2010. Blood. 116:5615. PubMed
  20. Cai B, et al. 2021. Mol Cancer. 20:165. PubMed
  21. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  22. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  23. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  24. Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
  25. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  26. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  27. Ontiveros-Padilla L, et al. 2021. Microorganisms. 9:. PubMed
  28. Wang Z, et al. 2020. Autophagy. 1:. PubMed
  29. Mishra P, et al. 2013. Brain Behav Immun. 34:98. PubMed
  30. Ma C, et al. 2016. Nature. 531:253-257. PubMed
  31. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  32. Xiong Y, et al. 2018. Int J Clin Exp Pathol. 3.803472222. PubMed
  33. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  34. Zongyi Y, et al. 2017. PLoS One. 10.1371/journal.pone.0189617. PubMed
  35. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  36. Shami A, et al. 2015. Sci Rep. 5: 13904. PubMed
  37. Shiomi A, et al. 2014. J Immunol. 193:849. PubMed
  38. Pedraza–Zamora CP, et al. 2017. Parasite Immunol. 39:4. PubMed
  39. Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  40. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  41. Bonnefoy F, et al. 2011. J Immunol. 186:5696. PubMed
  42. Wu H, et al. 2012. J Am Soc Nephrol. 23:1701. PubMed
  43. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  44. Chuang YM, et al. 2018. Front Cell Infect Microbiol. 8:31. PubMed
  45. Lee CG, et al. 2019. JCI Insight. 4:5. PubMed
  46. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  47. Dai G, et al. 2021. mSphere. 6: . PubMed
  48. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  49. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  50. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  51. Zanvit P, et al. 2015. Nat Commun. 6: 8424. PubMed
  52. Tian X, et al. 2022. J Cell Mol Med. 26:693. PubMed
  53. Liu B, et al. 2022. Mol Med Rep. 26:. PubMed
  54. Arbelaez C, et al. 2015. J Immunol. 195: 1974-1983. PubMed
  55. Kastelberg B, et al. 2020. PLoS Pathog. 16:e1008854. PubMed
  56. Jin C, et al. 2019. Cell. 176:998. PubMed
  57. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  58. Han F, et al. 2020. Invest Ophthalmol Vis Sci. 61:24. PubMed
  59. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  60. Ding Y, et al. 2020. Evid Based Complement Alternat Med. 2020:9521073. PubMed
  61. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  62. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  63. Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed
  64. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  65. Robb R, et al. 2011. Blood. 118:3399. PubMed
  66. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  67. Callejas BE, et al. 2021. Front Immunol. 12:744738. PubMed
  68. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  69. Meyer Zu Horste G et al. 2018. Immunity. 48(3):556-569 . PubMed
  70. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  71. Makino A, et al. 2021. iScience. 24:103201. PubMed
  72. Kiuchi M, et al. 2021. J Exp Med. 218:. PubMed
  73. Zhang H, et al. 2017. Immunol Cell Biol. 10.1038/icb.2017.11. PubMed
  74. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  75. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  76. Xu Y, et al. 2016. Nat Commun. 7:12073. PubMed
  77. Wang Y, et al. 2021. Front Immunol. 11:601601. PubMed
  78. Miranda PM, et al. 2018. Microbiome. 6:57. PubMed
  79. Webb LM, et al. 2020. J Clin Invest. 130:1683. PubMed
  80. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  81. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  82. Liu YW, et al. 2022. JCI Insight. 7:. PubMed
  83. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  84. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  85. Yue T, et al. 2022. Cell Death Differ. 29:218. PubMed
  86. Zhang X, et al. 2021. Front Pharmacol. 12:706748. PubMed
  87. Deng J, et al. 2021. EBioMedicine. 70:103505. PubMed
  88. Stevanin M, et al. 2017. Eur J Immunol. 10.1002/eji.201646565. PubMed
  89. Long L, et al. 2021. Nature. 600:308. PubMed
  90. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  91. Lytle NK et al. 2019. Cell. 177(3):572-586 . PubMed
  92. Nagashima H, et al. 2016. J Immunol. 196: 4082 - 4089. PubMed
  93. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  94. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  95. Miki H, et al. 2020. J Immunol. 204:1892. PubMed
  96. Zutshi S, et al. 2021. Immunology. 163:460. PubMed
  97. Yang L, et al. 2022. Acta Pharmacol Sin. 43:963. PubMed
  98. Wang YC, et al. 2023. J Exp Med. 220:. PubMed
  99. Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed
  100. Lebson L, et al. 2010. J Immunol. 185:7161. PubMed
  101. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  102. Su Y, et al. 2022. J Hematol Oncol. 15:99. PubMed
  103. Liu J, et al. 2019. Immunity. 50:600. PubMed
  104. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  105. Gerber AN, et al. 2021. Cell Rep. 37:109816. PubMed
  106. Manivasagam S, et al. 2022. J Immunol. 208:1341. PubMed
  107. Kenichi Shimada et al. 2018. Immunity. 49(5):873-885 . PubMed
  108. MaruYama T 2015. Biochem Biophys Res Commun. 464: 586-589. PubMed
  109. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  110. Andreas N, et al. 2021. Arthritis Res Ther. 23:222. PubMed
  111. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  112. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  113. Li J, et al. 2010. Blood. 116:5615. PubMed
  114. Cai B, et al. 2021. Mol Cancer. 20:165. PubMed
  115. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  116. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  117. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  118. Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
  119. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  120. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  121. Ontiveros-Padilla L, et al. 2021. Microorganisms. 9:. PubMed
  122. Wang Z, et al. 2020. Autophagy. 1:. PubMed
  123. Mishra P, et al. 2013. Brain Behav Immun. 34:98. PubMed
  124. Ma C, et al. 2016. Nature. 531:253-257. PubMed
  125. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  126. Xiong Y, et al. 2018. Int J Clin Exp Pathol. 3.803472222. PubMed
  127. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  128. Zongyi Y, et al. 2017. PLoS One. 10.1371/journal.pone.0189617. PubMed
  129. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  130. Shami A, et al. 2015. Sci Rep. 5: 13904. PubMed
  131. Shiomi A, et al. 2014. J Immunol. 193:849. PubMed
  132. Pedraza–Zamora CP, et al. 2017. Parasite Immunol. 39:4. PubMed
  133. Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  134. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  135. Bonnefoy F, et al. 2011. J Immunol. 186:5696. PubMed
  136. Wu H, et al. 2012. J Am Soc Nephrol. 23:1701. PubMed
  137. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  138. Chuang YM, et al. 2018. Front Cell Infect Microbiol. 8:31. PubMed
  139. Lee CG, et al. 2019. JCI Insight. 4:5. PubMed
  140. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  141. Dai G, et al. 2021. mSphere. 6: . PubMed
  142. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  143. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  144. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  145. Zanvit P, et al. 2015. Nat Commun. 6: 8424. PubMed
  146. Tian X, et al. 2022. J Cell Mol Med. 26:693. PubMed
  147. Liu B, et al. 2022. Mol Med Rep. 26:. PubMed
  148. Arbelaez C, et al. 2015. J Immunol. 195: 1974-1983. PubMed
  149. Kastelberg B, et al. 2020. PLoS Pathog. 16:e1008854. PubMed
  150. Jin C, et al. 2019. Cell. 176:998. PubMed
  151. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  152. Han F, et al. 2020. Invest Ophthalmol Vis Sci. 61:24. PubMed
  153. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  154. Ding Y, et al. 2020. Evid Based Complement Alternat Med. 2020:9521073. PubMed
  155. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  156. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  157. Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed
  158. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  159. Robb R, et al. 2011. Blood. 118:3399. PubMed
  160. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  161. Callejas BE, et al. 2021. Front Immunol. 12:744738. PubMed
  162. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  163. Meyer Zu Horste G et al. 2018. Immunity. 48(3):556-569 . PubMed
  164. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  165. Makino A, et al. 2021. iScience. 24:103201. PubMed
  166. Kiuchi M, et al. 2021. J Exp Med. 218:. PubMed
  167. Zhang H, et al. 2017. Immunol Cell Biol. 10.1038/icb.2017.11. PubMed
  168. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  169. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  170. Xu Y, et al. 2016. Nat Commun. 7:12073. PubMed
  171. Wang Y, et al. 2021. Front Immunol. 11:601601. PubMed
  172. Miranda PM, et al. 2018. Microbiome. 6:57. PubMed
  173. Webb LM, et al. 2020. J Clin Invest. 130:1683. PubMed
  174. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  175. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  176. Liu YW, et al. 2022. JCI Insight. 7:. PubMed
  177. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  178. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  179. Yue T, et al. 2022. Cell Death Differ. 29:218. PubMed
  180. Zhang X, et al. 2021. Front Pharmacol. 12:706748. PubMed
  181. Deng J, et al. 2021. EBioMedicine. 70:103505. PubMed
  182. Stevanin M, et al. 2017. Eur J Immunol. 10.1002/eji.201646565. PubMed
  183. Long L, et al. 2021. Nature. 600:308. PubMed
  184. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  185. Lytle NK et al. 2019. Cell. 177(3):572-586 . PubMed
  186. Nagashima H, et al. 2016. J Immunol. 196: 4082 - 4089. PubMed
  187. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  188. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
RRID
AB_536017 (BioLegend Cat. No. 506915)
AB_536018 (BioLegend Cat. No. 506916)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian).
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?
Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.
Go To Top Version: 2    Revision Date: 10/16/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
TC11-18H10.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
TC11-18H10_APC_031808
PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 APC
  • TC11-18H10_APC_031808
    PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
506915 25 µg DKK779
Check Availability


Need larger quantities of this item?
Request Bulk Quote
506916 100 µg DKK1759
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The IL-17A antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References
  1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
  2. Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
  3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
  4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
  5. Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
  6. Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
  7. Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
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  153. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  154. Ding Y, et al. 2020. Evid Based Complement Alternat Med. 2020:9521073. PubMed
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  161. Callejas BE, et al. 2021. Front Immunol. 12:744738. PubMed
  162. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
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  164. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
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  181. Deng J, et al. 2021. EBioMedicine. 70:103505. PubMed
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  183. Long L, et al. 2021. Nature. 600:308. PubMed
  184. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  185. Lytle NK et al. 2019. Cell. 177(3):572-586 . PubMed
  186. Nagashima H, et al. 2016. J Immunol. 196: 4082 - 4089. PubMed
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  188. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
RRID
AB_536017 (BioLegend Cat. No. 506915)
AB_536018 (BioLegend Cat. No. 506916)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian).
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?
Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.
Go To Top Version: 2    Revision Date: 10/16/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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