Loading...

APC/Cyanine7 anti-mouse CD8a Antibody APC/Cyanine7 anti-mouse CD8a Antibody

Pricing & Availability
Clone
53-6.7 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
53-6dot7_APCCyanine7_CD8_Antibody_013019_updated.png
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD8 (clone 53-6.7) APC/Cyanine7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
  • 53-6dot7_APCCyanine7_CD8_Antibody_013019_updated.png
    C57BL/6 mouse splenocytes were stained with CD3 FITC and CD8 (clone 53-6.7) APC/Cyanine7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
100713 25 µg DKK559
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100714 100 µg DKK1278
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD8a antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Application References
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
  29. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  30. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Cha JH et al. 2018. Molecular cell. 71(4):606-620 . PubMed
  2. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  3. Chan LC, et al. 2019. J Clin Invest. 129:3324. PubMed
  4. Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed
  5. Kastenmüller K, et al. 2013. Infect Immun . 81:789. PubMed
  6. Quinn K, et al. 2013. J Immunol. 191:5085. PubMed
  7. Chuang H, et al. 2014. Nat Commun. 5:4602. PubMed
  8. Cabrera-Mora M, et al. 2016. J Immunol. 197: 2748 - 2761. PubMed
  9. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  10. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  11. Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed
  12. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  13. Mills EL, et al. 2022. Cell Metab. 34:140. PubMed
  14. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  15. Chan WY, et al. 2022. Infect Immun. 90:e0084618a. PubMed
  16. Cha JH, et al. 2022. J Biol Chem. 298:101817. PubMed
  17. Qu L, et al. 2022. Cell. 185:1728. PubMed
  18. Tu J, et al. 2022. Cancer Sci. 113:2496. PubMed
  19. Kiani Shabestari S, et al. 2022. Cell Rep. 39:110961. PubMed
  20. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  21. Yu Z, et al. 2022. Biomater Sci. 11:263. PubMed
  22. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  23. Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed
  24. Azevedo PO, et al. 2023. NPJ Vaccines. 8:15. PubMed
  25. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  26. Kuehm LM, et al. 2021. Cancer Immunol Res. 9:227. PubMed
  27. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  28. Elazar A, et al. 2022. Elife. 11:. PubMed
  29. Berube A, et al. 2022. Viruses. 14:. PubMed
  30. Li T, et al. 2022. J Leukoc Biol. 112:845. PubMed
  31. Duhalde Vega M, et al. 2022. Sci Adv. 8:eabn6545. PubMed
  32. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  33. Ou W, et al. 2023. Nat Commun. 14:392. PubMed
  34. Zhu S, et al. 2023. Theranostics. 13:1042. PubMed
  35. Wang X, et al. 2023. J Immunother Cancer. 11:. PubMed
  36. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  37. Ke F, et al. 2023. Elife. 12:. PubMed
  38. Tursi NJ, et al. 2023. Front Immunol. 14:1072810. PubMed
  39. Rege SV, et al. 2023. Commun Biol. 6:292. PubMed
  40. Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed
  41. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  42. Tan X, et al. 2023. Adv Sci (Weinh). 10:e2206768. PubMed
  43. Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed
  44. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  45. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  46. Chen J, et al. 2008. Blood. 111:5163. PubMed
  47. Fu Z, et al. 2019. Cell Rep. 28:159. PubMed
  48. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  49. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
  50. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  51. Hirako I, et al. 2016. Nat Commun. 7:13277. PubMed
  52. Singhal A, et al. 2014. Sci Transl Med. 6:263. PubMed
  53. Hong M, et al. 2021. Int J Mol Sci. 22:. PubMed
  54. Qi Z, et al. 2022. Nat Commun. 13:182. PubMed
  55. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  56. Zhang P et al. 2019. Cell Rep. 27(11):3117-3123 . PubMed
  57. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  58. Spranger S, et al. 2013. Sci Transl Med. 26:200. PubMed
  59. Sanchez-Felipe L, et al. 2021. Nature. 590:320. PubMed
  60. Wei H, et al. 2021. Malar J. 20:89. PubMed
  61. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  62. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  63. Park JH, et al. 2022. STAR Protoc. 3:101607. PubMed
  64. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  65. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  66. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  67. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  68. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  69. Zhou Y, et al. 2016. Acta Pharmacol Sin. 10.1038/aps.2016.102. PubMed
  70. Jazowiecka-Rakus J, et al. 2021. Cancers (Basel). 13:. PubMed
  71. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  72. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  73. Lian G et al. 2018. eLife. 7 pii: e36158. PubMed
  74. Das S, et al. 2019. Oncogene. 383316. PubMed
  75. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
  76. Guo X, et al. 2020. Nat Commun. 11:2177. PubMed
  77. Somerville TD, et al. 2020. eLife. 9:e53381.. PubMed
  78. He C, et al. 2020. Clin Transl Med. 10:e39. PubMed
  79. Isakova-Sivak I, et al. 2020. Vaccines (Basel). 8:00. PubMed
  80. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  81. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  82. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  83. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  84. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  85. Konrath KM, et al. 2022. Cell Rep. 38:110318. PubMed
  86. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  87. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  88. Hsu JM, et al. 2018. Nat Commun. 9:1908. PubMed
  89. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  90. Marangoni F, et al. 2021. Cell. . PubMed
  91. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  92. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  93. Zhang X, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:494. PubMed
  94. Zhang C, et al. 2021. Clin Transl Immunology. 10:e1310. PubMed
  95. Nagarajan D, et al. 2021. Immunol Cell Biol. 99:972. PubMed
  96. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  97. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  98. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  99. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  100. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  101. Hiltensperger M, et al. 2021. Nat Immunol. 22:880. PubMed
  102. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  103. Wang B, et al. 2022. Nat Commun. 13:3821. PubMed
  104. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  105. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  106. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  107. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  108. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  109. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  110. Kang SJ, et al. 2022. Food Sci Anim Resour. 42:903. PubMed
  111. Muñoz NM, et al. 2022. Sci Rep. 12:14449. PubMed
  112. Li J, et al. 2021. Cell Commun Signal. 19:89. PubMed
  113. Soto-Díaz K, et al. 2021. Front Immunol. 12:734349. PubMed
  114. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  115. O'Brien S, Zhang M 2015. J Immunol. 195: 695-705. PubMed
  116. Zheng J, et al. 2020. bioRxiv. . PubMed
  117. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  118. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
  119. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  120. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  121. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  122. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  123. Flosbach M, et al. 2020. Cell Reports. 32(4):107957. PubMed
  124. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  125. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  126. Fu Z, et al. 2021. Nat Commun. 12:4462. PubMed
  127. França TT, et al. 2021. JCI Insight. 6:. PubMed
  128. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  129. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  130. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  131. Zheng J, et al. 2020. Nature. . PubMed
  132. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  133. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  134. Gough D, et al. 2014. Blood. 124:2252. PubMed
  135. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  136. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  137. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  138. Merlo LM, et al. 2020. Clin Pathol. 13:2632010X20951812. PubMed
  139. Li J, et al. 2013. Arch Immunol Ther Exp (Warsz). 61:237. PubMed
  140. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  141. Giampazolias E, et al. 2021. Cell. . PubMed
  142. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  143. Martin MD, et al. 2020. Cell Rep. 31:107508. PubMed
  144. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  145. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  146. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
  147. Wang Z, et al. 2021. Acta Pharm Sin B. 694:11. PubMed
  148. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  149. Hou Y, et al. 2021. Sci Transl Med. 13:. PubMed
  150. Ye Q, et al. 2022. NPJ Vaccines. 7:84. PubMed
  151. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  152. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  153. Yasuma K, et al. 2016. PLoS Pathog. 12: 1005372. PubMed
  154. Fang Y, et al. 2021. J Clin Invest. 131:00:00. PubMed
  155. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  156. Zhang J, et al. 2022. Biomater Res. 26:44. PubMed
  157. Jazowiecka-Rakus J, et al. 2020. Mol Ther Oncolytics. 18:335. PubMed
  158. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  159. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  160. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  161. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  162. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  163. Shoji M, et al. 2012. PLoS One. 7:e30302. PubMed
  164. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  165. Koide S, et al. 2022. iScience. 25:103603. PubMed
  166. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  167. Tsunekuni K, et al. 2020. J Clin Med. 9:00. PubMed
  168. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  169. Pradhan K, et al. 2021. J Immunol. 206:2980. PubMed
  170. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  171. Neckermann P, et al. 2021. Front Immunol. 12:761214. PubMed
  172. Chew HY, et al. 2020. Cell. 180(5):895-914. PubMed
  173. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  174. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  175. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  176. Oh J, et al. 2021. Clin Cancer Res. 27:4781. PubMed
  177. Li H, et al. 2022. iScience. 25:104481. PubMed
  178. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  179. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  180. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  181. Zhang B, et al. 2021. Nature. 599:471. PubMed
  182. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  183. Yang FM, et al. 2022. Front Immunol. 13:918241. PubMed
  184. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  185. Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
  186. Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed
  187. Lynn GM, et al. 2020. Nat Biotechnol. 38:320. PubMed
  188. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  189. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  190. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  191. Yang H, et al. 2022. Viruses. 14:. PubMed
  192. Bessell CA, et al. 2020. JCI Insight. 5:. PubMed
  193. Li H, et al. 2021. Signal Transduct Target Ther. 6:389. PubMed
  194. Cha JH et al. 2018. Molecular cell. 71(4):606-620 . PubMed
  195. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  196. Chan LC, et al. 2019. J Clin Invest. 129:3324. PubMed
  197. Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed
  198. Kastenmüller K, et al. 2013. Infect Immun . 81:789. PubMed
  199. Quinn K, et al. 2013. J Immunol. 191:5085. PubMed
  200. Chuang H, et al. 2014. Nat Commun. 5:4602. PubMed
  201. Cabrera-Mora M, et al. 2016. J Immunol. 197: 2748 - 2761. PubMed
  202. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  203. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  204. Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed
  205. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  206. Mills EL, et al. 2022. Cell Metab. 34:140. PubMed
  207. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  208. Chan WY, et al. 2022. Infect Immun. 90:e0084618a. PubMed
  209. Cha JH, et al. 2022. J Biol Chem. 298:101817. PubMed
  210. Qu L, et al. 2022. Cell. 185:1728. PubMed
  211. Tu J, et al. 2022. Cancer Sci. 113:2496. PubMed
  212. Kiani Shabestari S, et al. 2022. Cell Rep. 39:110961. PubMed
  213. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  214. Yu Z, et al. 2022. Biomater Sci. 11:263. PubMed
  215. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  216. Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed
  217. Azevedo PO, et al. 2023. NPJ Vaccines. 8:15. PubMed
  218. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  219. Kuehm LM, et al. 2021. Cancer Immunol Res. 9:227. PubMed
  220. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  221. Elazar A, et al. 2022. Elife. 11:. PubMed
  222. Berube A, et al. 2022. Viruses. 14:. PubMed
  223. Li T, et al. 2022. J Leukoc Biol. 112:845. PubMed
  224. Duhalde Vega M, et al. 2022. Sci Adv. 8:eabn6545. PubMed
  225. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  226. Ou W, et al. 2023. Nat Commun. 14:392. PubMed
  227. Zhu S, et al. 2023. Theranostics. 13:1042. PubMed
  228. Wang X, et al. 2023. J Immunother Cancer. 11:. PubMed
  229. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  230. Ke F, et al. 2023. Elife. 12:. PubMed
  231. Tursi NJ, et al. 2023. Front Immunol. 14:1072810. PubMed
  232. Rege SV, et al. 2023. Commun Biol. 6:292. PubMed
  233. Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed
  234. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  235. Tan X, et al. 2023. Adv Sci (Weinh). 10:e2206768. PubMed
  236. Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed
  237. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  238. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  239. Chen J, et al. 2008. Blood. 111:5163. PubMed
  240. Fu Z, et al. 2019. Cell Rep. 28:159. PubMed
  241. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  242. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
  243. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  244. Hirako I, et al. 2016. Nat Commun. 7:13277. PubMed
  245. Singhal A, et al. 2014. Sci Transl Med. 6:263. PubMed
  246. Hong M, et al. 2021. Int J Mol Sci. 22:. PubMed
  247. Qi Z, et al. 2022. Nat Commun. 13:182. PubMed
  248. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  249. Zhang P et al. 2019. Cell Rep. 27(11):3117-3123 . PubMed
  250. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  251. Spranger S, et al. 2013. Sci Transl Med. 26:200. PubMed
  252. Sanchez-Felipe L, et al. 2021. Nature. 590:320. PubMed
  253. Wei H, et al. 2021. Malar J. 20:89. PubMed
  254. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  255. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  256. Park JH, et al. 2022. STAR Protoc. 3:101607. PubMed
  257. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  258. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  259. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  260. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  261. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  262. Zhou Y, et al. 2016. Acta Pharmacol Sin. 10.1038/aps.2016.102. PubMed
  263. Jazowiecka-Rakus J, et al. 2021. Cancers (Basel). 13:. PubMed
  264. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  265. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  266. Lian G et al. 2018. eLife. 7 pii: e36158. PubMed
  267. Das S, et al. 2019. Oncogene. 383316. PubMed
  268. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
  269. Guo X, et al. 2020. Nat Commun. 11:2177. PubMed
  270. Somerville TD, et al. 2020. eLife. 9:e53381.. PubMed
  271. He C, et al. 2020. Clin Transl Med. 10:e39. PubMed
  272. Isakova-Sivak I, et al. 2020. Vaccines (Basel). 8:00. PubMed
  273. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  274. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  275. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  276. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  277. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  278. Konrath KM, et al. 2022. Cell Rep. 38:110318. PubMed
  279. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  280. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  281. Hsu JM, et al. 2018. Nat Commun. 9:1908. PubMed
  282. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  283. Marangoni F, et al. 2021. Cell. . PubMed
  284. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  285. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  286. Zhang X, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:494. PubMed
  287. Zhang C, et al. 2021. Clin Transl Immunology. 10:e1310. PubMed
  288. Nagarajan D, et al. 2021. Immunol Cell Biol. 99:972. PubMed
  289. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  290. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  291. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  292. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  293. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  294. Hiltensperger M, et al. 2021. Nat Immunol. 22:880. PubMed
  295. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  296. Wang B, et al. 2022. Nat Commun. 13:3821. PubMed
  297. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  298. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  299. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  300. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  301. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  302. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  303. Kang SJ, et al. 2022. Food Sci Anim Resour. 42:903. PubMed
  304. Muñoz NM, et al. 2022. Sci Rep. 12:14449. PubMed
  305. Li J, et al. 2021. Cell Commun Signal. 19:89. PubMed
  306. Soto-Díaz K, et al. 2021. Front Immunol. 12:734349. PubMed
  307. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  308. O'Brien S, Zhang M 2015. J Immunol. 195: 695-705. PubMed
  309. Zheng J, et al. 2020. bioRxiv. . PubMed
  310. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  311. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
  312. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  313. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  314. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  315. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  316. Flosbach M, et al. 2020. Cell Reports. 32(4):107957. PubMed
  317. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  318. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  319. Fu Z, et al. 2021. Nat Commun. 12:4462. PubMed
  320. França TT, et al. 2021. JCI Insight. 6:. PubMed
  321. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  322. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  323. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  324. Zheng J, et al. 2020. Nature. . PubMed
  325. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  326. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  327. Gough D, et al. 2014. Blood. 124:2252. PubMed
  328. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  329. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  330. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  331. Merlo LM, et al. 2020. Clin Pathol. 13:2632010X20951812. PubMed
  332. Li J, et al. 2013. Arch Immunol Ther Exp (Warsz). 61:237. PubMed
  333. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  334. Giampazolias E, et al. 2021. Cell. . PubMed
  335. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  336. Martin MD, et al. 2020. Cell Rep. 31:107508. PubMed
  337. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  338. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  339. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
  340. Wang Z, et al. 2021. Acta Pharm Sin B. 694:11. PubMed
  341. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  342. Hou Y, et al. 2021. Sci Transl Med. 13:. PubMed
  343. Ye Q, et al. 2022. NPJ Vaccines. 7:84. PubMed
  344. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  345. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  346. Yasuma K, et al. 2016. PLoS Pathog. 12: 1005372. PubMed
  347. Fang Y, et al. 2021. J Clin Invest. 131:00:00. PubMed
  348. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  349. Zhang J, et al. 2022. Biomater Res. 26:44. PubMed
  350. Jazowiecka-Rakus J, et al. 2020. Mol Ther Oncolytics. 18:335. PubMed
  351. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  352. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  353. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  354. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  355. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  356. Shoji M, et al. 2012. PLoS One. 7:e30302. PubMed
  357. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  358. Koide S, et al. 2022. iScience. 25:103603. PubMed
  359. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  360. Tsunekuni K, et al. 2020. J Clin Med. 9:00. PubMed
  361. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  362. Pradhan K, et al. 2021. J Immunol. 206:2980. PubMed
  363. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  364. Neckermann P, et al. 2021. Front Immunol. 12:761214. PubMed
  365. Chew HY, et al. 2020. Cell. 180(5):895-914. PubMed
  366. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  367. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  368. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  369. Oh J, et al. 2021. Clin Cancer Res. 27:4781. PubMed
  370. Li H, et al. 2022. iScience. 25:104481. PubMed
  371. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  372. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  373. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  374. Zhang B, et al. 2021. Nature. 599:471. PubMed
  375. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  376. Yang FM, et al. 2022. Front Immunol. 13:918241. PubMed
  377. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  378. Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
  379. Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed
  380. Lynn GM, et al. 2020. Nat Biotechnol. 38:320. PubMed
  381. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  382. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  383. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  384. Yang H, et al. 2022. Viruses. 14:. PubMed
  385. Bessell CA, et al. 2020. JCI Insight. 5:. PubMed
  386. Li H, et al. 2021. Signal Transduct Target Ther. 6:389. PubMed
RRID
AB_312752 (BioLegend Cat. No. 100713)
AB_312753 (BioLegend Cat. No. 100714)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Other Formats

View All CD8a Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD8a 53-6.7 FC
Biotin anti-mouse CD8a 53-6.7 FC,IHC
FITC anti-mouse CD8a 53-6.7 FC
PE anti-mouse CD8a 53-6.7 FC
PE/Cyanine5 anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC-F,IP
PE/Cyanine7 anti-mouse CD8a 53-6.7 FC
APC/Cyanine7 anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 488 anti-mouse CD8a 53-6.7 FC,3D IHC
Alexa Fluor® 647 anti-mouse CD8a 53-6.7 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 700 anti-mouse CD8a 53-6.7 FC
PerCP/Cyanine5.5 anti-mouse CD8a 53-6.7 FC
PerCP anti-mouse CD8a 53-6.7 FC
Brilliant Violet 421™ anti-mouse CD8a 53-6.7 FC,IHC,SB
Brilliant Violet 570™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 650™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 605™ anti-mouse CD8a 53-6.7 FC
Ultra-LEAF™ Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC,IP,Depletion,Block
Brilliant Violet 711™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 785™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 510™ anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a (Maxpar® Ready) 53-6.7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD8a 53-6.7 IHC-F,FC,3D IHC
PE/Dazzle™ 594 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 750 anti-mouse CD8a 53-6.7 FC
GoInVivo™ Purified anti-mouse CD8a 53-6.7 FC
TotalSeq™-A0002 anti-mouse CD8a 53-6.7 PG
Spark Blue™ 550 anti-mouse CD8a 53-6.7 FC
Spark NIR™ 685 anti-mouse CD8a 53-6.7 FC
TotalSeq™-C0002 anti-mouse CD8a 53-6.7 PG
TotalSeq™-B0002 anti-mouse CD8a 53-6.7 PG
Spark YG™ 570 anti-mouse CD8a 53-6.7 IHC-F
PE/Fire™ 640 anti-mouse CD8a 53-6.7 FC
PE/Fire™ 700 anti-mouse CD8a 53-6.7 FC
Spark Blue™ 574 anti-mouse CD8a Antibody 53-6.7 FC
Spark Violet™ 423 anti-mouse CD8a Antibody 53-6.7 FC
Spark UV™ 387 anti-mouse CD8a 53-6.7 FC
Spark Blue™ 515 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark Red™ 718 anti-mouse CD8a (Flexi-Fluor™) 53-6.7 FC
PE/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark PLUS UV395™ anti-mouse CD8a 53-6.7 FC
PerCP/Fire™ 780 anti-mouse CD8a 53-6.7 FC
Spark YG™ 581 anti-mouse CD8a (Flexi-Fluor™) 53-6.7 FC
Spark YG™ 593 anti-mouse CD8a (Flexi-Fluor™) Antibody 53-6.7 FC
StarBright UltraViolet 575 anti-mouse CD8a 53-6.7 FC
Spark PLUS V475™ anti-mouse CD8a 53-6.7 FC
Go To Top Version: 3    Revision Date: 01/30/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
53-6.7 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
53-6dot7_APCCyanine7_CD8_Antibody_013019_updated.png
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD8 (clone 53-6.7) APC/Cyanine7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
  • 53-6dot7_APCCyanine7_CD8_Antibody_013019_updated.png
    C57BL/6 mouse splenocytes were stained with CD3 FITC and CD8 (clone 53-6.7) APC/Cyanine7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
100713 25 µg DKK559
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100714 100 µg DKK1278
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD8a antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Application References
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
  29. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  30. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Cha JH et al. 2018. Molecular cell. 71(4):606-620 . PubMed
  2. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  3. Chan LC, et al. 2019. J Clin Invest. 129:3324. PubMed
  4. Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed
  5. Kastenmüller K, et al. 2013. Infect Immun . 81:789. PubMed
  6. Quinn K, et al. 2013. J Immunol. 191:5085. PubMed
  7. Chuang H, et al. 2014. Nat Commun. 5:4602. PubMed
  8. Cabrera-Mora M, et al. 2016. J Immunol. 197: 2748 - 2761. PubMed
  9. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  10. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  11. Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed
  12. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  13. Mills EL, et al. 2022. Cell Metab. 34:140. PubMed
  14. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  15. Chan WY, et al. 2022. Infect Immun. 90:e0084618a. PubMed
  16. Cha JH, et al. 2022. J Biol Chem. 298:101817. PubMed
  17. Qu L, et al. 2022. Cell. 185:1728. PubMed
  18. Tu J, et al. 2022. Cancer Sci. 113:2496. PubMed
  19. Kiani Shabestari S, et al. 2022. Cell Rep. 39:110961. PubMed
  20. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  21. Yu Z, et al. 2022. Biomater Sci. 11:263. PubMed
  22. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  23. Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed
  24. Azevedo PO, et al. 2023. NPJ Vaccines. 8:15. PubMed
  25. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  26. Kuehm LM, et al. 2021. Cancer Immunol Res. 9:227. PubMed
  27. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  28. Elazar A, et al. 2022. Elife. 11:. PubMed
  29. Berube A, et al. 2022. Viruses. 14:. PubMed
  30. Li T, et al. 2022. J Leukoc Biol. 112:845. PubMed
  31. Duhalde Vega M, et al. 2022. Sci Adv. 8:eabn6545. PubMed
  32. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  33. Ou W, et al. 2023. Nat Commun. 14:392. PubMed
  34. Zhu S, et al. 2023. Theranostics. 13:1042. PubMed
  35. Wang X, et al. 2023. J Immunother Cancer. 11:. PubMed
  36. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  37. Ke F, et al. 2023. Elife. 12:. PubMed
  38. Tursi NJ, et al. 2023. Front Immunol. 14:1072810. PubMed
  39. Rege SV, et al. 2023. Commun Biol. 6:292. PubMed
  40. Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed
  41. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  42. Tan X, et al. 2023. Adv Sci (Weinh). 10:e2206768. PubMed
  43. Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed
  44. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  45. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  46. Chen J, et al. 2008. Blood. 111:5163. PubMed
  47. Fu Z, et al. 2019. Cell Rep. 28:159. PubMed
  48. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  49. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
  50. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  51. Hirako I, et al. 2016. Nat Commun. 7:13277. PubMed
  52. Singhal A, et al. 2014. Sci Transl Med. 6:263. PubMed
  53. Hong M, et al. 2021. Int J Mol Sci. 22:. PubMed
  54. Qi Z, et al. 2022. Nat Commun. 13:182. PubMed
  55. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  56. Zhang P et al. 2019. Cell Rep. 27(11):3117-3123 . PubMed
  57. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  58. Spranger S, et al. 2013. Sci Transl Med. 26:200. PubMed
  59. Sanchez-Felipe L, et al. 2021. Nature. 590:320. PubMed
  60. Wei H, et al. 2021. Malar J. 20:89. PubMed
  61. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  62. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  63. Park JH, et al. 2022. STAR Protoc. 3:101607. PubMed
  64. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  65. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  66. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  67. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  68. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  69. Zhou Y, et al. 2016. Acta Pharmacol Sin. 10.1038/aps.2016.102. PubMed
  70. Jazowiecka-Rakus J, et al. 2021. Cancers (Basel). 13:. PubMed
  71. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  72. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  73. Lian G et al. 2018. eLife. 7 pii: e36158. PubMed
  74. Das S, et al. 2019. Oncogene. 383316. PubMed
  75. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
  76. Guo X, et al. 2020. Nat Commun. 11:2177. PubMed
  77. Somerville TD, et al. 2020. eLife. 9:e53381.. PubMed
  78. He C, et al. 2020. Clin Transl Med. 10:e39. PubMed
  79. Isakova-Sivak I, et al. 2020. Vaccines (Basel). 8:00. PubMed
  80. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  81. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  82. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  83. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  84. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  85. Konrath KM, et al. 2022. Cell Rep. 38:110318. PubMed
  86. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  87. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  88. Hsu JM, et al. 2018. Nat Commun. 9:1908. PubMed
  89. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  90. Marangoni F, et al. 2021. Cell. . PubMed
  91. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  92. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  93. Zhang X, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:494. PubMed
  94. Zhang C, et al. 2021. Clin Transl Immunology. 10:e1310. PubMed
  95. Nagarajan D, et al. 2021. Immunol Cell Biol. 99:972. PubMed
  96. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  97. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  98. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  99. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  100. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  101. Hiltensperger M, et al. 2021. Nat Immunol. 22:880. PubMed
  102. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  103. Wang B, et al. 2022. Nat Commun. 13:3821. PubMed
  104. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  105. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  106. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  107. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  108. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  109. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  110. Kang SJ, et al. 2022. Food Sci Anim Resour. 42:903. PubMed
  111. Muñoz NM, et al. 2022. Sci Rep. 12:14449. PubMed
  112. Li J, et al. 2021. Cell Commun Signal. 19:89. PubMed
  113. Soto-Díaz K, et al. 2021. Front Immunol. 12:734349. PubMed
  114. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  115. O'Brien S, Zhang M 2015. J Immunol. 195: 695-705. PubMed
  116. Zheng J, et al. 2020. bioRxiv. . PubMed
  117. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  118. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
  119. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  120. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  121. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  122. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  123. Flosbach M, et al. 2020. Cell Reports. 32(4):107957. PubMed
  124. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  125. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  126. Fu Z, et al. 2021. Nat Commun. 12:4462. PubMed
  127. França TT, et al. 2021. JCI Insight. 6:. PubMed
  128. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  129. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  130. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  131. Zheng J, et al. 2020. Nature. . PubMed
  132. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  133. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  134. Gough D, et al. 2014. Blood. 124:2252. PubMed
  135. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  136. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  137. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  138. Merlo LM, et al. 2020. Clin Pathol. 13:2632010X20951812. PubMed
  139. Li J, et al. 2013. Arch Immunol Ther Exp (Warsz). 61:237. PubMed
  140. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  141. Giampazolias E, et al. 2021. Cell. . PubMed
  142. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  143. Martin MD, et al. 2020. Cell Rep. 31:107508. PubMed
  144. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  145. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  146. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
  147. Wang Z, et al. 2021. Acta Pharm Sin B. 694:11. PubMed
  148. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  149. Hou Y, et al. 2021. Sci Transl Med. 13:. PubMed
  150. Ye Q, et al. 2022. NPJ Vaccines. 7:84. PubMed
  151. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  152. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  153. Yasuma K, et al. 2016. PLoS Pathog. 12: 1005372. PubMed
  154. Fang Y, et al. 2021. J Clin Invest. 131:00:00. PubMed
  155. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  156. Zhang J, et al. 2022. Biomater Res. 26:44. PubMed
  157. Jazowiecka-Rakus J, et al. 2020. Mol Ther Oncolytics. 18:335. PubMed
  158. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  159. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  160. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  161. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  162. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  163. Shoji M, et al. 2012. PLoS One. 7:e30302. PubMed
  164. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  165. Koide S, et al. 2022. iScience. 25:103603. PubMed
  166. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  167. Tsunekuni K, et al. 2020. J Clin Med. 9:00. PubMed
  168. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  169. Pradhan K, et al. 2021. J Immunol. 206:2980. PubMed
  170. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  171. Neckermann P, et al. 2021. Front Immunol. 12:761214. PubMed
  172. Chew HY, et al. 2020. Cell. 180(5):895-914. PubMed
  173. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  174. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  175. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  176. Oh J, et al. 2021. Clin Cancer Res. 27:4781. PubMed
  177. Li H, et al. 2022. iScience. 25:104481. PubMed
  178. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  179. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  180. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  181. Zhang B, et al. 2021. Nature. 599:471. PubMed
  182. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  183. Yang FM, et al. 2022. Front Immunol. 13:918241. PubMed
  184. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  185. Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
  186. Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed
  187. Lynn GM, et al. 2020. Nat Biotechnol. 38:320. PubMed
  188. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  189. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  190. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  191. Yang H, et al. 2022. Viruses. 14:. PubMed
  192. Bessell CA, et al. 2020. JCI Insight. 5:. PubMed
  193. Li H, et al. 2021. Signal Transduct Target Ther. 6:389. PubMed
  194. Cha JH et al. 2018. Molecular cell. 71(4):606-620 . PubMed
  195. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  196. Chan LC, et al. 2019. J Clin Invest. 129:3324. PubMed
  197. Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed
  198. Kastenmüller K, et al. 2013. Infect Immun . 81:789. PubMed
  199. Quinn K, et al. 2013. J Immunol. 191:5085. PubMed
  200. Chuang H, et al. 2014. Nat Commun. 5:4602. PubMed
  201. Cabrera-Mora M, et al. 2016. J Immunol. 197: 2748 - 2761. PubMed
  202. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  203. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  204. Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed
  205. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  206. Mills EL, et al. 2022. Cell Metab. 34:140. PubMed
  207. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  208. Chan WY, et al. 2022. Infect Immun. 90:e0084618a. PubMed
  209. Cha JH, et al. 2022. J Biol Chem. 298:101817. PubMed
  210. Qu L, et al. 2022. Cell. 185:1728. PubMed
  211. Tu J, et al. 2022. Cancer Sci. 113:2496. PubMed
  212. Kiani Shabestari S, et al. 2022. Cell Rep. 39:110961. PubMed
  213. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  214. Yu Z, et al. 2022. Biomater Sci. 11:263. PubMed
  215. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  216. Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed
  217. Azevedo PO, et al. 2023. NPJ Vaccines. 8:15. PubMed
  218. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  219. Kuehm LM, et al. 2021. Cancer Immunol Res. 9:227. PubMed
  220. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  221. Elazar A, et al. 2022. Elife. 11:. PubMed
  222. Berube A, et al. 2022. Viruses. 14:. PubMed
  223. Li T, et al. 2022. J Leukoc Biol. 112:845. PubMed
  224. Duhalde Vega M, et al. 2022. Sci Adv. 8:eabn6545. PubMed
  225. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  226. Ou W, et al. 2023. Nat Commun. 14:392. PubMed
  227. Zhu S, et al. 2023. Theranostics. 13:1042. PubMed
  228. Wang X, et al. 2023. J Immunother Cancer. 11:. PubMed
  229. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  230. Ke F, et al. 2023. Elife. 12:. PubMed
  231. Tursi NJ, et al. 2023. Front Immunol. 14:1072810. PubMed
  232. Rege SV, et al. 2023. Commun Biol. 6:292. PubMed
  233. Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed
  234. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  235. Tan X, et al. 2023. Adv Sci (Weinh). 10:e2206768. PubMed
  236. Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed
  237. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  238. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  239. Chen J, et al. 2008. Blood. 111:5163. PubMed
  240. Fu Z, et al. 2019. Cell Rep. 28:159. PubMed
  241. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  242. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
  243. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  244. Hirako I, et al. 2016. Nat Commun. 7:13277. PubMed
  245. Singhal A, et al. 2014. Sci Transl Med. 6:263. PubMed
  246. Hong M, et al. 2021. Int J Mol Sci. 22:. PubMed
  247. Qi Z, et al. 2022. Nat Commun. 13:182. PubMed
  248. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  249. Zhang P et al. 2019. Cell Rep. 27(11):3117-3123 . PubMed
  250. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  251. Spranger S, et al. 2013. Sci Transl Med. 26:200. PubMed
  252. Sanchez-Felipe L, et al. 2021. Nature. 590:320. PubMed
  253. Wei H, et al. 2021. Malar J. 20:89. PubMed
  254. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  255. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  256. Park JH, et al. 2022. STAR Protoc. 3:101607. PubMed
  257. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  258. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  259. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  260. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  261. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  262. Zhou Y, et al. 2016. Acta Pharmacol Sin. 10.1038/aps.2016.102. PubMed
  263. Jazowiecka-Rakus J, et al. 2021. Cancers (Basel). 13:. PubMed
  264. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  265. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  266. Lian G et al. 2018. eLife. 7 pii: e36158. PubMed
  267. Das S, et al. 2019. Oncogene. 383316. PubMed
  268. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
  269. Guo X, et al. 2020. Nat Commun. 11:2177. PubMed
  270. Somerville TD, et al. 2020. eLife. 9:e53381.. PubMed
  271. He C, et al. 2020. Clin Transl Med. 10:e39. PubMed
  272. Isakova-Sivak I, et al. 2020. Vaccines (Basel). 8:00. PubMed
  273. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  274. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  275. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  276. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  277. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  278. Konrath KM, et al. 2022. Cell Rep. 38:110318. PubMed
  279. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  280. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  281. Hsu JM, et al. 2018. Nat Commun. 9:1908. PubMed
  282. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  283. Marangoni F, et al. 2021. Cell. . PubMed
  284. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  285. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  286. Zhang X, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:494. PubMed
  287. Zhang C, et al. 2021. Clin Transl Immunology. 10:e1310. PubMed
  288. Nagarajan D, et al. 2021. Immunol Cell Biol. 99:972. PubMed
  289. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  290. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  291. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  292. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  293. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  294. Hiltensperger M, et al. 2021. Nat Immunol. 22:880. PubMed
  295. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  296. Wang B, et al. 2022. Nat Commun. 13:3821. PubMed
  297. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  298. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  299. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  300. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  301. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  302. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  303. Kang SJ, et al. 2022. Food Sci Anim Resour. 42:903. PubMed
  304. Muñoz NM, et al. 2022. Sci Rep. 12:14449. PubMed
  305. Li J, et al. 2021. Cell Commun Signal. 19:89. PubMed
  306. Soto-Díaz K, et al. 2021. Front Immunol. 12:734349. PubMed
  307. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  308. O'Brien S, Zhang M 2015. J Immunol. 195: 695-705. PubMed
  309. Zheng J, et al. 2020. bioRxiv. . PubMed
  310. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  311. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
  312. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  313. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  314. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  315. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  316. Flosbach M, et al. 2020. Cell Reports. 32(4):107957. PubMed
  317. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  318. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  319. Fu Z, et al. 2021. Nat Commun. 12:4462. PubMed
  320. França TT, et al. 2021. JCI Insight. 6:. PubMed
  321. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  322. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  323. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  324. Zheng J, et al. 2020. Nature. . PubMed
  325. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  326. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  327. Gough D, et al. 2014. Blood. 124:2252. PubMed
  328. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  329. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  330. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  331. Merlo LM, et al. 2020. Clin Pathol. 13:2632010X20951812. PubMed
  332. Li J, et al. 2013. Arch Immunol Ther Exp (Warsz). 61:237. PubMed
  333. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  334. Giampazolias E, et al. 2021. Cell. . PubMed
  335. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  336. Martin MD, et al. 2020. Cell Rep. 31:107508. PubMed
  337. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  338. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  339. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
  340. Wang Z, et al. 2021. Acta Pharm Sin B. 694:11. PubMed
  341. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  342. Hou Y, et al. 2021. Sci Transl Med. 13:. PubMed
  343. Ye Q, et al. 2022. NPJ Vaccines. 7:84. PubMed
  344. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  345. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  346. Yasuma K, et al. 2016. PLoS Pathog. 12: 1005372. PubMed
  347. Fang Y, et al. 2021. J Clin Invest. 131:00:00. PubMed
  348. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  349. Zhang J, et al. 2022. Biomater Res. 26:44. PubMed
  350. Jazowiecka-Rakus J, et al. 2020. Mol Ther Oncolytics. 18:335. PubMed
  351. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  352. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  353. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  354. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  355. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  356. Shoji M, et al. 2012. PLoS One. 7:e30302. PubMed
  357. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  358. Koide S, et al. 2022. iScience. 25:103603. PubMed
  359. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  360. Tsunekuni K, et al. 2020. J Clin Med. 9:00. PubMed
  361. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  362. Pradhan K, et al. 2021. J Immunol. 206:2980. PubMed
  363. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  364. Neckermann P, et al. 2021. Front Immunol. 12:761214. PubMed
  365. Chew HY, et al. 2020. Cell. 180(5):895-914. PubMed
  366. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  367. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  368. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  369. Oh J, et al. 2021. Clin Cancer Res. 27:4781. PubMed
  370. Li H, et al. 2022. iScience. 25:104481. PubMed
  371. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  372. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  373. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  374. Zhang B, et al. 2021. Nature. 599:471. PubMed
  375. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  376. Yang FM, et al. 2022. Front Immunol. 13:918241. PubMed
  377. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  378. Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
  379. Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed
  380. Lynn GM, et al. 2020. Nat Biotechnol. 38:320. PubMed
  381. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  382. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  383. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  384. Yang H, et al. 2022. Viruses. 14:. PubMed
  385. Bessell CA, et al. 2020. JCI Insight. 5:. PubMed
  386. Li H, et al. 2021. Signal Transduct Target Ther. 6:389. PubMed
RRID
AB_312752 (BioLegend Cat. No. 100713)
AB_312753 (BioLegend Cat. No. 100714)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Other Formats

View All CD8a Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD8a 53-6.7 FC
Biotin anti-mouse CD8a 53-6.7 FC,IHC
FITC anti-mouse CD8a 53-6.7 FC
PE anti-mouse CD8a 53-6.7 FC
PE/Cyanine5 anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC-F,IP
PE/Cyanine7 anti-mouse CD8a 53-6.7 FC
APC/Cyanine7 anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 488 anti-mouse CD8a 53-6.7 FC,3D IHC
Alexa Fluor® 647 anti-mouse CD8a 53-6.7 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 700 anti-mouse CD8a 53-6.7 FC
PerCP/Cyanine5.5 anti-mouse CD8a 53-6.7 FC
PerCP anti-mouse CD8a 53-6.7 FC
Brilliant Violet 421™ anti-mouse CD8a 53-6.7 FC,IHC,SB
Brilliant Violet 570™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 650™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 605™ anti-mouse CD8a 53-6.7 FC
Ultra-LEAF™ Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC,IP,Depletion,Block
Brilliant Violet 711™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 785™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 510™ anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a (Maxpar® Ready) 53-6.7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD8a 53-6.7 IHC-F,FC,3D IHC
PE/Dazzle™ 594 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 750 anti-mouse CD8a 53-6.7 FC
GoInVivo™ Purified anti-mouse CD8a 53-6.7 FC
TotalSeq™-A0002 anti-mouse CD8a 53-6.7 PG
Spark Blue™ 550 anti-mouse CD8a 53-6.7 FC
Spark NIR™ 685 anti-mouse CD8a 53-6.7 FC
TotalSeq™-C0002 anti-mouse CD8a 53-6.7 PG
TotalSeq™-B0002 anti-mouse CD8a 53-6.7 PG
Spark YG™ 570 anti-mouse CD8a 53-6.7 IHC-F
PE/Fire™ 640 anti-mouse CD8a 53-6.7 FC
PE/Fire™ 700 anti-mouse CD8a 53-6.7 FC
Spark Blue™ 574 anti-mouse CD8a Antibody 53-6.7 FC
Spark Violet™ 423 anti-mouse CD8a Antibody 53-6.7 FC
Spark UV™ 387 anti-mouse CD8a 53-6.7 FC
Spark Blue™ 515 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark Red™ 718 anti-mouse CD8a (Flexi-Fluor™) 53-6.7 FC
PE/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark PLUS UV395™ anti-mouse CD8a 53-6.7 FC
PerCP/Fire™ 780 anti-mouse CD8a 53-6.7 FC
Spark YG™ 581 anti-mouse CD8a (Flexi-Fluor™) 53-6.7 FC
Spark YG™ 593 anti-mouse CD8a (Flexi-Fluor™) Antibody 53-6.7 FC
StarBright UltraViolet 575 anti-mouse CD8a 53-6.7 FC
Spark PLUS V475™ anti-mouse CD8a 53-6.7 FC
Go To Top Version: 3    Revision Date: 01/30/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account