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Biotin anti-mouse CD69 Antibody Biotin anti-mouse CD69 Antibody

Pricing & Availability
Clone
H1.2F3 (See other available formats)
Regulatory Status
RUO
Other Names
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
H1dot2F3_Biotin_091907
Con A-stimulated Balb/c mouse splenocytes (24 hours) stained with biotinylated H1.2F3, followed by Sav-PE
  • H1dot2F3_Biotin_091907
    Con A-stimulated Balb/c mouse splenocytes (24 hours) stained with biotinylated H1.2F3, followed by Sav-PE
Compare all formats
Cat # Size Price Quantity Check Availability Save
104503 50 µg DKK422
Check Availability


Need larger quantities of this item?
Request Bulk Quote
104504 500 µg DKK1622
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse dendritic epidermal T cell line Y245
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.

This antibody has been characterized in the literature as containing a lambda light chain.

Application References
  1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
  2. Sobel ES, et al. 1993. J. Immunol. 150:673.
  3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
  5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  7. Lee JW, et al. 2006. Nature Immunol. 8:181.
  8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
  9. Jordan JM, et al. 2008. 76:3717. PubMed
  10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
Product Citations
  1. Xu RH, et al. 2015. Immunity. 1148:43. PubMed
  2. Hu Z, et al. 2015. J Exp Med. 101084/jm20150178. PubMed
  3. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  4. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  5. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  6. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  7. Sood S, et al. 2016. J Immunol. 197: 429 - 440. PubMed
  8. Xu RH, et al. 2015. Immunity. 1148:43. PubMed
  9. Hu Z, et al. 2015. J Exp Med. 101084/jm20150178. PubMed
  10. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  11. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  12. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  13. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  14. Sood S, et al. 2016. J Immunol. 197: 429 - 440. PubMed
RRID
AB_313106 (BioLegend Cat. No. 104503)
AB_313107 (BioLegend Cat. No. 104504)

Antigen Details

Structure
C-type lectin, 27/33 kD
Distribution

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

Function
Lymphocyte activation
Cell Type
B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Gene ID
12515 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD69 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD69 H1.2F3 FC
FITC anti-mouse CD69 H1.2F3 FC
PE anti-mouse CD69 H1.2F3 FC
PE/Cyanine5 anti-mouse CD69 H1.2F3 FC
Purified anti-mouse CD69 H1.2F3 FC,IHC-F,IP
PE/Cyanine7 anti-mouse CD69 H1.2F3 FC
APC anti-mouse CD69 H1.2F3 FC
Alexa Fluor® 488 anti-mouse CD69 H1.2F3 FC
Alexa Fluor® 647 anti-mouse CD69 H1.2F3 FC
PerCP anti-mouse CD69 H1.2F3 FC
PerCP/Cyanine5.5 anti-mouse CD69 H1.2F3 FC
Pacific Blue™ anti-mouse CD69 H1.2F3 FC
Brilliant Violet 421™ anti-mouse CD69 H1.2F3 FC
APC/Cyanine7 anti-mouse CD69 H1.2F3 FC
Brilliant Violet 605™ anti-mouse CD69 H1.2F3 FC
Brilliant Violet 510™ anti-mouse CD69 H1.2F3 FC
Purified anti-mouse CD69 (Maxpar® Ready) H1.2F3 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD69 H1.2F3 FC
Brilliant Violet 711™ anti-mouse CD69 H1.2F3 FC
Alexa Fluor® 700 anti-mouse CD69 H1.2F3 FC
Brilliant Violet 650™ anti-mouse CD69 H1.2F3 FC
Brilliant Violet 785™ anti-mouse CD69 H1.2F3 FC
TotalSeq™-A0197 anti-mouse CD69 H1.2F3 PG
APC/Fire™ 750 anti-mouse CD69 H1.2F3 FC
TotalSeq™-C0197 anti-mouse CD69 H1.2F3 PG
TotalSeq™-B0197 anti-mouse CD69 H1.2F3 PG
KIRAVIA Blue 520™ anti-mouse CD69 H1.2F3 FC
Spark NIR™ 685 anti-mouse CD69 H1.2F3 FC
Spark Red™ 718 anti-mouse CD69 H1.2F3 FC
Spark Blue™ 574 anti-mouse CD69 (Flexi-Fluor™) H1.2F3 FC
Spark PLUS B550™ anti-mouse CD69 H1.2F3 FC
Spark PLUS UV395™ anti-mouse CD69 Antibody H1.2F3 FC
Spark YG™ 581 anti-mouse CD69 (Flexi-Fluor™) H1.2F3 FC
Spark YG™ 593 anti-mouse CD69 (Flexi-Fluor™) Antibody H1.2F3 FC
Spark UV™ 387 anti-mouse CD69 (Flexi-Fluor™) H1.2F3 FC
Go To Top Version: 3    Revision Date: 04/18/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
H1.2F3 (See other available formats)
Regulatory Status
RUO
Other Names
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
H1dot2F3_Biotin_091907
Con A-stimulated Balb/c mouse splenocytes (24 hours) stained with biotinylated H1.2F3, followed by Sav-PE
  • H1dot2F3_Biotin_091907
    Con A-stimulated Balb/c mouse splenocytes (24 hours) stained with biotinylated H1.2F3, followed by Sav-PE
Compare all formats
Cat # Size Price Quantity Check Availability Save
104503 50 µg DKK422
Check Availability


Need larger quantities of this item?
Request Bulk Quote
104504 500 µg DKK1622
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse dendritic epidermal T cell line Y245
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.

This antibody has been characterized in the literature as containing a lambda light chain.

Application References
  1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
  2. Sobel ES, et al. 1993. J. Immunol. 150:673.
  3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
  5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  7. Lee JW, et al. 2006. Nature Immunol. 8:181.
  8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
  9. Jordan JM, et al. 2008. 76:3717. PubMed
  10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
Product Citations
  1. Xu RH, et al. 2015. Immunity. 1148:43. PubMed
  2. Hu Z, et al. 2015. J Exp Med. 101084/jm20150178. PubMed
  3. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  4. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  5. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  6. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  7. Sood S, et al. 2016. J Immunol. 197: 429 - 440. PubMed
  8. Xu RH, et al. 2015. Immunity. 1148:43. PubMed
  9. Hu Z, et al. 2015. J Exp Med. 101084/jm20150178. PubMed
  10. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  11. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  12. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  13. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  14. Sood S, et al. 2016. J Immunol. 197: 429 - 440. PubMed
RRID
AB_313106 (BioLegend Cat. No. 104503)
AB_313107 (BioLegend Cat. No. 104504)

Antigen Details

Structure
C-type lectin, 27/33 kD
Distribution

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

Function
Lymphocyte activation
Cell Type
B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Gene ID
12515 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD69 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD69 H1.2F3 FC
FITC anti-mouse CD69 H1.2F3 FC
PE anti-mouse CD69 H1.2F3 FC
PE/Cyanine5 anti-mouse CD69 H1.2F3 FC
Purified anti-mouse CD69 H1.2F3 FC,IHC-F,IP
PE/Cyanine7 anti-mouse CD69 H1.2F3 FC
APC anti-mouse CD69 H1.2F3 FC
Alexa Fluor® 488 anti-mouse CD69 H1.2F3 FC
Alexa Fluor® 647 anti-mouse CD69 H1.2F3 FC
PerCP anti-mouse CD69 H1.2F3 FC
PerCP/Cyanine5.5 anti-mouse CD69 H1.2F3 FC
Pacific Blue™ anti-mouse CD69 H1.2F3 FC
Brilliant Violet 421™ anti-mouse CD69 H1.2F3 FC
APC/Cyanine7 anti-mouse CD69 H1.2F3 FC
Brilliant Violet 605™ anti-mouse CD69 H1.2F3 FC
Brilliant Violet 510™ anti-mouse CD69 H1.2F3 FC
Purified anti-mouse CD69 (Maxpar® Ready) H1.2F3 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD69 H1.2F3 FC
Brilliant Violet 711™ anti-mouse CD69 H1.2F3 FC
Alexa Fluor® 700 anti-mouse CD69 H1.2F3 FC
Brilliant Violet 650™ anti-mouse CD69 H1.2F3 FC
Brilliant Violet 785™ anti-mouse CD69 H1.2F3 FC
TotalSeq™-A0197 anti-mouse CD69 H1.2F3 PG
APC/Fire™ 750 anti-mouse CD69 H1.2F3 FC
TotalSeq™-C0197 anti-mouse CD69 H1.2F3 PG
TotalSeq™-B0197 anti-mouse CD69 H1.2F3 PG
KIRAVIA Blue 520™ anti-mouse CD69 H1.2F3 FC
Spark NIR™ 685 anti-mouse CD69 H1.2F3 FC
Spark Red™ 718 anti-mouse CD69 H1.2F3 FC
Spark Blue™ 574 anti-mouse CD69 (Flexi-Fluor™) H1.2F3 FC
Spark PLUS B550™ anti-mouse CD69 H1.2F3 FC
Spark PLUS UV395™ anti-mouse CD69 Antibody H1.2F3 FC
Spark YG™ 581 anti-mouse CD69 (Flexi-Fluor™) H1.2F3 FC
Spark YG™ 593 anti-mouse CD69 (Flexi-Fluor™) Antibody H1.2F3 FC
Spark UV™ 387 anti-mouse CD69 (Flexi-Fluor™) H1.2F3 FC
Go To Top Version: 3    Revision Date: 04/18/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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