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ELISA MAX™ Deluxe Set Mouse TNF-α ELISA MAX™ Deluxe Set Mouse TNF-α

Pricing & Availability
Regulatory Status
RUO
Other Names
TNF-a ELISA Kit
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Product Citations
publications
mouse tnf-a_122109
  • mouse tnf-a_122109
Cat # Size Price Quantity Check Availability Save
430904 5 plates DKK1812
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430915 10 Plates DKK3481
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430916 20 Plates DKK5572
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Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • Mouse TNF-α ELISA MAX™ Capture Antibody (200X)
  • Mouse TNF-α ELISA MAX™ Detection Antibody (200X)
  • Mouse TNF-α Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References
  1. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273.
  2. ChavantF, et al. 2010. J. Pharmacol Exp. Ther. 332:505. PubMed
  3. Lewis CJ, et al. 2010. J. Immunol. 184:3789. PubMed
  4. Qui J, et al. Appl Environ Microbiol. PubMed
  5. Yang HJ, et al. 2011. J Cell Sci. 124:2552. PubMed.
  6. White LE, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  7. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  8. Zachman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  9. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  10. Zahman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  11. Justice JN, et al. 2015. J appl Physiol. 118:163. PubMed
Product Citations
  1. Luo W, et al. 2018. Bioorg Med Chem. 26:1547. PubMed
  2. Dong X, et al. 2019. ACS Nano. 13:1272. PubMed
  3. Tsai YR, et al. 2022. Pharmaceutics. 14:. PubMed
  4. Cho MJ, et al. 2023. Exp Mol Med. 55:1033. PubMed
  5. Foster SL, et al. 2023. Cells. 12:. PubMed
  6. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  7. Gao J, et al. 2023. Pharmaceutics. 15:. PubMed
  8. Guo W, et al. 2021. Int J Biol Sci. 17:4271. PubMed
  9. Chou WC, et al. 2021. Nature. 591:300. PubMed
  10. Ishikawa S, et al. 2021. Biol Pharm Bull. 44:550. PubMed
  11. Cerrato G, et al. 2021. Cell Death Differ. 28:1733. PubMed
  12. Ahangar P, et al. 2021. Wound Repair Regen. 29:380. PubMed
  13. Wang JJ, et al. 2022. J Biol Chem. 298:102017. PubMed
  14. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  15. Yu M, et al. 2022. Front Pharmacol. 13:985618. PubMed
  16. Cheng P, et al. 2023. Pharmacol Res. 188:106643. PubMed
  17. Zhao C, et al. 2023. PLoS Pathog. 19:e1011108. PubMed
  18. Wei K, et al. 2023. Signal Transduct Target Ther. 8:22. PubMed
  19. Sun XY, et al. 2023. Signal Transduct Target Ther. 8:30. PubMed
  20. Zhao C, et al. 2023. NPJ Biofilms Microbiomes. 9:8. PubMed
  21. Hsu CG, et al. 2022. Cell Death Differ. 29:1790. PubMed
  22. Shih S, et al. 2016. PLoS One. 11: 0157647. PubMed
  23. Kim MW, et al. 2020. Front Bioeng Biotechnol. 8:133. PubMed
  24. Li DY, et al. 2018. Sci Rep. 8:17979. PubMed
  25. Chen M, et al. 2022. J Nanobiotechnology. 20:273. PubMed
  26. Radwanska M, et al. 2021. Vaccines (Basel). 9:. PubMed
  27. Nagata K, et al. 2020. Int Immunol. 32:143. PubMed
  28. Du Q, et al. 2019. Front Microbiol. 10:2050. PubMed
  29. Yang Y, et al. 2020. J Int Med Res. 48:300060520902605. PubMed
  30. Ma X, et al. 2022. Cell Mol Immunol. . PubMed
  31. Kalafatakis I, et al. 2021. J Neurosci Res. 99:1474. PubMed
  32. Zachman A, et al. 2014. Biomaterials. 35:9635. PubMed
  33. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  34. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  35. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  36. Li H, et al. 2021. Mol Med Rep. 1:23. PubMed
  37. Clausen B, et al. 2016. Sci Rep. 6:29291. PubMed
  38. Zhang B, et al. 2021. Exp Ther Med. 22:832. PubMed
  39. Lv ZY, et al. 2022. J Inflamm Res. 15:2819. PubMed
  40. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  41. Ye H, et al. 2016. Exp Mol Pathol. 100: 307-311. PubMed
  42. Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed
  43. Liu X, et al. 2021. Int J Mol Sci. 22:. PubMed
  44. Ahangar P, et al. 2020. NPJ Regen Med. 24:5. PubMed
  45. Brunetti J, et al. 2016. J Biol Chem. 291(49):25742-25748. PubMed
  46. Yang H, et al. 2011. J Cell Sci. 124:2552. PubMed
  47. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  48. Schaafsma W, et al. 2017. Neurobiology of Disease. 10.1016/j.nbd.2017.07.017. PubMed
  49. Shaker ME, et al. 2022. Saudi Pharm J. 30:291. PubMed
  50. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  51. Park GT, et al. 2021. Mar Drugs. 19:. PubMed
  52. Lin CC, et al. 2021. Iran J Basic Med Sci. 24:1146. PubMed
  53. Li Y, et al. 2021. J Neurochem. 159:867. PubMed
  54. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  55. Qiu J, et al. 2010. Appl Envir Microbiol. 76:5846. PubMed
  56. Dai X, et al. 2017. FASEB J. 31:2881. PubMed
  57. Liu R, et al. 2018. Exp Ther Med. 15:3385. PubMed
  58. Sousa AH, et al. 2021. Can J Physiol Pharmacol. 99:1324. PubMed
  59. Hsu CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  60. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  61. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  62. Iannucci J, et al. 2020. Cell Mol Neurobiol. . PubMed
  63. Liu TH, et al. 2018. Nutrients. 10:E472. PubMed
  64. Medrano–Jiménez E, et al. 2019. J Neuroinflammation. 16:143. PubMed
  65. Park GT, et al. 2019. Front Immunol. 10:2095. PubMed
  66. Yu Y, et al. 2021. Cell Rep. 37:110126. PubMed
  67. Wang J, et al. 2022. J Clin Invest. :. PubMed
  68. Lee N, et al. 2022. Front Immunol. 13:940258. PubMed
  69. Hsueh SC, et al. 2022. Biomedicines. 10:. PubMed
  70. Al Mamun A, et al. 2020. Proc Natl Acad Sci U S A. 117:1742. PubMed
  71. Ci T, et al. 2020. Sci Adv. 6:00. PubMed
  72. Wilson JL, et al. 2020. Cell Rep. 30:1542. PubMed
  73. Xiahou Z,et al. 2017. Nat Commun. . 10.1038/s41467-017-00930-9. PubMed
  74. Qi S, et al. 2022. Transl Stroke Res. :. PubMed
  75. Hsu CG, et al. 2021. J Immunol. 206:3010. PubMed
  76. Fan Q, et al. 2021. Bioact Mater. 6:4014. PubMed
  77. Zhou XM, et al. 2018. Front Pharmacol. 8:904. PubMed
  78. Sun B, et al. 2018. Cell Mol Immunol. 1.168055556. PubMed
  79. Li WS, et al. 2021. Int J Mol Sci. 22:. PubMed
  80. Nuro-Gyina PK, et al. 2021. J Immunol. 207:868. PubMed
  81. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  82. Wang C, et al. 2020. Redox Biol. 37:101688. PubMed
  83. White L, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  84. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  85. Justice J, et al. 2015. J Appl Physiol. 118:163. PubMed
  86. Chan K, et al. 2015. J Biol Chem. 290: 16979 - 16988. PubMed
  87. Stanfield BA, et al. 2021. PLoS One. 16:e0245169. PubMed
  88. Lanfermann C, et al. 2021. Vaccines (Basel). 9:. PubMed
  89. Feng L, et al. 2022. Signal Transduct Target Ther. 7:21. PubMed
  90. Yang FM, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  91. Pan Y, et al. 2021. Br J Pharmacol. 178:4907. PubMed
  92. Scanu A, et al. 2022. Int J Mol Sci. 23:. PubMed
  93. Deng LR, et al. 2022. Chin Med. 17:132. PubMed
  94. Schaafsma W, et al. 2015. Brain Behav Immun. 48: 205-221. PubMed
  95. Nagata K, et al. 2021. Front Immunol. 12:730706. PubMed
  96. Gu H, et al. 2021. Elife. 10:. PubMed
  97. Zhuo X, et al. 2021. Biomed Rep. 15:64. PubMed
  98. Lanfermann C, et al. 2021. Vaccines (Basel). 9:. PubMed
  99. de León-Guerrero SD, et al. 2022. Dis Model Mech. . PubMed
  100. Su S, et al. 2022. Signal Transduct Target Ther. 7:137. PubMed
  101. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  102. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  103. Li X, et al. 2022. Sci Rep. 12:17058. PubMed
  104. Agrahari G, et al. 2021. Front Immunol. 12:628117. PubMed
  105. Cobb C 2010. J Immunol. 184:3789. PubMed
  106. Chavant F, et al. 2010. J Pharmacol Exp Ther. 332:505. PubMed
  107. Reed MD, et al. 2020. Nature. 577:249. PubMed
  108. Fante T, et al. 2016. PLoS One. 11: 0160184. PubMed
  109. Chen Y, et al. 2018. Exp Ther Med. 16:4322. PubMed
  110. Ratna A, et al. 2021. Hepatol Commun. 5:1165. PubMed
  111. Luo W, et al. 2018. Bioorg Med Chem. 26:1547. PubMed
  112. Dong X, et al. 2019. ACS Nano. 13:1272. PubMed
  113. Tsai YR, et al. 2022. Pharmaceutics. 14:. PubMed
  114. Cho MJ, et al. 2023. Exp Mol Med. 55:1033. PubMed
  115. Foster SL, et al. 2023. Cells. 12:. PubMed
  116. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  117. Gao J, et al. 2023. Pharmaceutics. 15:. PubMed
  118. Guo W, et al. 2021. Int J Biol Sci. 17:4271. PubMed
  119. Chou WC, et al. 2021. Nature. 591:300. PubMed
  120. Ishikawa S, et al. 2021. Biol Pharm Bull. 44:550. PubMed
  121. Cerrato G, et al. 2021. Cell Death Differ. 28:1733. PubMed
  122. Ahangar P, et al. 2021. Wound Repair Regen. 29:380. PubMed
  123. Wang JJ, et al. 2022. J Biol Chem. 298:102017. PubMed
  124. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  125. Yu M, et al. 2022. Front Pharmacol. 13:985618. PubMed
  126. Cheng P, et al. 2023. Pharmacol Res. 188:106643. PubMed
  127. Zhao C, et al. 2023. PLoS Pathog. 19:e1011108. PubMed
  128. Wei K, et al. 2023. Signal Transduct Target Ther. 8:22. PubMed
  129. Sun XY, et al. 2023. Signal Transduct Target Ther. 8:30. PubMed
  130. Zhao C, et al. 2023. NPJ Biofilms Microbiomes. 9:8. PubMed
  131. Hsu CG, et al. 2022. Cell Death Differ. 29:1790. PubMed
  132. Shih S, et al. 2016. PLoS One. 11: 0157647. PubMed
  133. Kim MW, et al. 2020. Front Bioeng Biotechnol. 8:133. PubMed
  134. Li DY, et al. 2018. Sci Rep. 8:17979. PubMed
  135. Chen M, et al. 2022. J Nanobiotechnology. 20:273. PubMed
  136. Radwanska M, et al. 2021. Vaccines (Basel). 9:. PubMed
  137. Nagata K, et al. 2020. Int Immunol. 32:143. PubMed
  138. Du Q, et al. 2019. Front Microbiol. 10:2050. PubMed
  139. Yang Y, et al. 2020. J Int Med Res. 48:300060520902605. PubMed
  140. Ma X, et al. 2022. Cell Mol Immunol. . PubMed
  141. Kalafatakis I, et al. 2021. J Neurosci Res. 99:1474. PubMed
  142. Zachman A, et al. 2014. Biomaterials. 35:9635. PubMed
  143. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  144. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  145. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  146. Li H, et al. 2021. Mol Med Rep. 1:23. PubMed
  147. Clausen B, et al. 2016. Sci Rep. 6:29291. PubMed
  148. Zhang B, et al. 2021. Exp Ther Med. 22:832. PubMed
  149. Lv ZY, et al. 2022. J Inflamm Res. 15:2819. PubMed
  150. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  151. Ye H, et al. 2016. Exp Mol Pathol. 100: 307-311. PubMed
  152. Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed
  153. Liu X, et al. 2021. Int J Mol Sci. 22:. PubMed
  154. Ahangar P, et al. 2020. NPJ Regen Med. 24:5. PubMed
  155. Brunetti J, et al. 2016. J Biol Chem. 291(49):25742-25748. PubMed
  156. Yang H, et al. 2011. J Cell Sci. 124:2552. PubMed
  157. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  158. Schaafsma W, et al. 2017. Neurobiology of Disease. 10.1016/j.nbd.2017.07.017. PubMed
  159. Shaker ME, et al. 2022. Saudi Pharm J. 30:291. PubMed
  160. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  161. Park GT, et al. 2021. Mar Drugs. 19:. PubMed
  162. Lin CC, et al. 2021. Iran J Basic Med Sci. 24:1146. PubMed
  163. Li Y, et al. 2021. J Neurochem. 159:867. PubMed
  164. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  165. Qiu J, et al. 2010. Appl Envir Microbiol. 76:5846. PubMed
  166. Dai X, et al. 2017. FASEB J. 31:2881. PubMed
  167. Liu R, et al. 2018. Exp Ther Med. 15:3385. PubMed
  168. Sousa AH, et al. 2021. Can J Physiol Pharmacol. 99:1324. PubMed
  169. Hsu CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  170. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  171. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  172. Iannucci J, et al. 2020. Cell Mol Neurobiol. . PubMed
  173. Liu TH, et al. 2018. Nutrients. 10:E472. PubMed
  174. Medrano–Jiménez E, et al. 2019. J Neuroinflammation. 16:143. PubMed
  175. Park GT, et al. 2019. Front Immunol. 10:2095. PubMed
  176. Yu Y, et al. 2021. Cell Rep. 37:110126. PubMed
  177. Wang J, et al. 2022. J Clin Invest. :. PubMed
  178. Lee N, et al. 2022. Front Immunol. 13:940258. PubMed
  179. Hsueh SC, et al. 2022. Biomedicines. 10:. PubMed
  180. Al Mamun A, et al. 2020. Proc Natl Acad Sci U S A. 117:1742. PubMed
  181. Ci T, et al. 2020. Sci Adv. 6:00. PubMed
  182. Wilson JL, et al. 2020. Cell Rep. 30:1542. PubMed
  183. Xiahou Z,et al. 2017. Nat Commun. . 10.1038/s41467-017-00930-9. PubMed
  184. Qi S, et al. 2022. Transl Stroke Res. :. PubMed
  185. Hsu CG, et al. 2021. J Immunol. 206:3010. PubMed
  186. Fan Q, et al. 2021. Bioact Mater. 6:4014. PubMed
  187. Zhou XM, et al. 2018. Front Pharmacol. 8:904. PubMed
  188. Sun B, et al. 2018. Cell Mol Immunol. 1.168055556. PubMed
  189. Li WS, et al. 2021. Int J Mol Sci. 22:. PubMed
  190. Nuro-Gyina PK, et al. 2021. J Immunol. 207:868. PubMed
  191. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  192. Wang C, et al. 2020. Redox Biol. 37:101688. PubMed
  193. White L, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  194. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  195. Justice J, et al. 2015. J Appl Physiol. 118:163. PubMed
  196. Chan K, et al. 2015. J Biol Chem. 290: 16979 - 16988. PubMed
  197. Stanfield BA, et al. 2021. PLoS One. 16:e0245169. PubMed
  198. Lanfermann C, et al. 2021. Vaccines (Basel). 9:. PubMed
  199. Feng L, et al. 2022. Signal Transduct Target Ther. 7:21. PubMed
  200. Yang FM, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  201. Pan Y, et al. 2021. Br J Pharmacol. 178:4907. PubMed
  202. Scanu A, et al. 2022. Int J Mol Sci. 23:. PubMed
  203. Deng LR, et al. 2022. Chin Med. 17:132. PubMed
  204. Schaafsma W, et al. 2015. Brain Behav Immun. 48: 205-221. PubMed
  205. Nagata K, et al. 2021. Front Immunol. 12:730706. PubMed
  206. Gu H, et al. 2021. Elife. 10:. PubMed
  207. Zhuo X, et al. 2021. Biomed Rep. 15:64. PubMed
  208. Lanfermann C, et al. 2021. Vaccines (Basel). 9:. PubMed
  209. de León-Guerrero SD, et al. 2022. Dis Model Mech. . PubMed
  210. Su S, et al. 2022. Signal Transduct Target Ther. 7:137. PubMed
  211. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  212. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  213. Li X, et al. 2022. Sci Rep. 12:17058. PubMed
  214. Agrahari G, et al. 2021. Front Immunol. 12:628117. PubMed
  215. Cobb C 2010. J Immunol. 184:3789. PubMed
  216. Chavant F, et al. 2010. J Pharmacol Exp Ther. 332:505. PubMed
  217. Reed MD, et al. 2020. Nature. 577:249. PubMed
  218. Fante T, et al. 2016. PLoS One. 11: 0160184. PubMed
  219. Chen Y, et al. 2018. Exp Ther Med. 16:4322. PubMed
  220. Ratna A, et al. 2021. Hepatol Commun. 5:1165. PubMed
Sensitivity
4 pg/mL
Standard Range
7.8-500 pg/mL

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 4    Revision Date: 12/14/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Pricing & Availability
Regulatory Status
RUO
Other Names
TNF-a ELISA Kit
Ave. Rating
Submit a Review
Product Citations
publications
mouse tnf-a_122109
  • mouse tnf-a_122109
Cat # Size Price Quantity Check Availability Save
430904 5 plates DKK1812
Check Availability


Need larger quantities of this item?
Request Bulk Quote
430915 10 Plates DKK3481
Check Availability


Need larger quantities of this item?
Request Bulk Quote
430916 20 Plates DKK5572
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • Mouse TNF-α ELISA MAX™ Capture Antibody (200X)
  • Mouse TNF-α ELISA MAX™ Detection Antibody (200X)
  • Mouse TNF-α Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References
  1. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273.
  2. ChavantF, et al. 2010. J. Pharmacol Exp. Ther. 332:505. PubMed
  3. Lewis CJ, et al. 2010. J. Immunol. 184:3789. PubMed
  4. Qui J, et al. Appl Environ Microbiol. PubMed
  5. Yang HJ, et al. 2011. J Cell Sci. 124:2552. PubMed.
  6. White LE, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  7. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  8. Zachman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  9. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  10. Zahman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  11. Justice JN, et al. 2015. J appl Physiol. 118:163. PubMed
Product Citations
  1. Luo W, et al. 2018. Bioorg Med Chem. 26:1547. PubMed
  2. Dong X, et al. 2019. ACS Nano. 13:1272. PubMed
  3. Tsai YR, et al. 2022. Pharmaceutics. 14:. PubMed
  4. Cho MJ, et al. 2023. Exp Mol Med. 55:1033. PubMed
  5. Foster SL, et al. 2023. Cells. 12:. PubMed
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  215. Cobb C 2010. J Immunol. 184:3789. PubMed
  216. Chavant F, et al. 2010. J Pharmacol Exp Ther. 332:505. PubMed
  217. Reed MD, et al. 2020. Nature. 577:249. PubMed
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Sensitivity
4 pg/mL
Standard Range
7.8-500 pg/mL

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 4    Revision Date: 12/14/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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