CD3/CD28-stimulated human PBMCs (3 days) were treated with 10 mM 2-Deoxy-D-Glucose (2-DG), 1 µM Oligomycin A, a combination of both, or vehicle (Control) for 15 minutes at 37°C. Cells were then stained with ATP Red (Cat. No. 421944) and the ATP Red fluorescence intensity of CD3+ T cells was analyzed by flow cytometry. Error bars represent standard deviation from 3 different donors.
CD3/CD28-stimulated human PBMCs (3 days) were treated with 10 mM 2-Deoxy-D-Glucose (2-DG), 1 µM Oligomycin A, a combination of both, or vehicle (Control) for 15 minutes at 37°C. Cells were then stained with ATP Red (Cat. No. 421944) and the ATP Red fluorescence intensity of CD3+ T cells was analyzed by flow cytometry. Error bars represent standard deviation from 3 different donors.
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427509
500 µg
DKK357
Description
Oligomycin A suppresses oxidative phosphorylation in mitochondria by inhibiting ATP biosynthesis. Oligomycin A binds to the proton channel on the F0 component of ATP synthase, decoupling the F0 and F1 factors of the ATP synthase complex and blocking the electron transport chain. This effect is useful for investigations including oxidative phosphorylation and other studies of cell metabolism.
Solubility in DMSO: 100 mg/mL or 126.41 mM. Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.
Resuspend 1 mg of lyophilized Oligomycin A in 250 µL DMSO or 100% ethanol to make a 5 mM stock solution. For the 500 µg size, resuspend in 125 µl DMSO or 100% ethanol to make a 5 mM stock solution.
Storage & Handling
Store lyophilized powder at -20°C, away from moisture. Once reconstituted, store aliquoted at -80°C. Use reconstituted material within 6 months to avoid loss of potency. Avoid repeated freeze-thaw cycles.
For inhibition of oxidative phosphorylation in primary immune cells (e.g. PBMCs or splenocytes) a range from 0.25 µM to 1.0 µM is recommended. It is recommended that the reagent be titrated for each experiment to determine optimal performance.
CD3/CD28-stimulated human PBMCs (3 days) were treated with 10 mM 2-Deoxy-D-Glucose (2-DG), 1 µM Oligomycin A, a combination of both, or vehicle (Control) for 15 minutes at 37°C. Cells were then stained with ATP Red (Cat. No. 421944) and the ATP Red fluorescence intensity of CD3+ T cells was analyzed by flow cytometry. Error bars represent standard deviation from 3 different donors.
CD3/CD28-stimulated human PBMCs (3 days) were treated with 10 mM 2-Deoxy-D-Glucose (2-DG), 1 µM Oligomycin A, a combination of both, or vehicle (Control) for 15 minutes at 37°C. Cells were then stained with ATP Red (Cat. No. 421944) and the ATP Red fluorescence intensity of CD3+ T cells was analyzed by flow cytometry. Error bars represent standard deviation from 3 different donors.
Cat #
Size
Price
Quantity
Check Availability
Save
Input string was not in a correct format.
427509
500 µg
DKK357
Description
Oligomycin A suppresses oxidative phosphorylation in mitochondria by inhibiting ATP biosynthesis. Oligomycin A binds to the proton channel on the F0 component of ATP synthase, decoupling the F0 and F1 factors of the ATP synthase complex and blocking the electron transport chain. This effect is useful for investigations including oxidative phosphorylation and other studies of cell metabolism.
Solubility in DMSO: 100 mg/mL or 126.41 mM. Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.
Resuspend 1 mg of lyophilized Oligomycin A in 250 µL DMSO or 100% ethanol to make a 5 mM stock solution. For the 500 µg size, resuspend in 125 µl DMSO or 100% ethanol to make a 5 mM stock solution.
Storage & Handling
Store lyophilized powder at -20°C, away from moisture. Once reconstituted, store aliquoted at -80°C. Use reconstituted material within 6 months to avoid loss of potency. Avoid repeated freeze-thaw cycles.
For inhibition of oxidative phosphorylation in primary immune cells (e.g. PBMCs or splenocytes) a range from 0.25 µM to 1.0 µM is recommended. It is recommended that the reagent be titrated for each experiment to determine optimal performance.
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