Lung carcinoma cell line, A549, was starved in serum-free, glucose-free media and treated with 1 µM Rapamycin or left untreated. After 6 hours, cells were stained with Autophagy Detection Probe Blue (Cat. No. 421906), and the fluorescence intensity was quantified using Image J. Data shown are averages of at least 3 biological replicates. Error bars represent standard deviation.
Lung carcinoma cell line, A549, was starved in serum-free, glucose-free media and treated with 1 µM Rapamycin or left untreated. After 6 hours, cells were stained with Autophagy Detection Probe Blue (Cat. No. 421906), and the fluorescence intensity was quantified using Image J. Data shown are averages of at least 3 biological replicates. Error bars represent standard deviation.
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427504
1 kit
DKK1188
Description
Rapamycin is an antifungal metabolite produced by Streptomyces hygroscopicus. Rapamycin is a potent and specific mTOR inhibitor. This allosteric inhibitor of mammalian mTORC1 induces autophagy and also acts as an immunosuppressant. mTORC1 is a central integrator of nutrient signaling pathways and stimulates cell growth and proliferation by activating anabolic processes inhibiting catabolic process such as autophagy.
1 vial of Rapamycin (10mg, lyophilized), 1 vial of DMSO
Preparation
Solubility: DMSO: 18.28 mg/mL (20 mM)
Preparation of stock solution: Resuspend 10 mg of lyophilized Rapamycin in 546.9 µL of fresh DMSO to make a 20 mM stock solution
Storage & Handling
Store lyophilized powder at -20°C. Protect from light. Once reconstituted, store aliquoted at -80°C. Use reconstituted material within 6 months to avoid loss of potency.
Rapamycin was used for induction of Autophagy in a range from 1 to 40 µM. It is recommended that the reagent be titrated for each experiment to determine optimal performance.
Lung carcinoma cell line, A549, was starved in serum-free, glucose-free media and treated with 1 µM Rapamycin or left untreated. After 6 hours, cells were stained with Autophagy Detection Probe Blue (Cat. No. 421906), and the fluorescence intensity was quantified using Image J. Data shown are averages of at least 3 biological replicates. Error bars represent standard deviation.
Lung carcinoma cell line, A549, was starved in serum-free, glucose-free media and treated with 1 µM Rapamycin or left untreated. After 6 hours, cells were stained with Autophagy Detection Probe Blue (Cat. No. 421906), and the fluorescence intensity was quantified using Image J. Data shown are averages of at least 3 biological replicates. Error bars represent standard deviation.
Cat #
Size
Price
Quantity
Check Availability
Save
Input string was not in a correct format.
427504
1 kit
DKK1188
Description
Rapamycin is an antifungal metabolite produced by Streptomyces hygroscopicus. Rapamycin is a potent and specific mTOR inhibitor. This allosteric inhibitor of mammalian mTORC1 induces autophagy and also acts as an immunosuppressant. mTORC1 is a central integrator of nutrient signaling pathways and stimulates cell growth and proliferation by activating anabolic processes inhibiting catabolic process such as autophagy.
1 vial of Rapamycin (10mg, lyophilized), 1 vial of DMSO
Preparation
Solubility: DMSO: 18.28 mg/mL (20 mM)
Preparation of stock solution: Resuspend 10 mg of lyophilized Rapamycin in 546.9 µL of fresh DMSO to make a 20 mM stock solution
Storage & Handling
Store lyophilized powder at -20°C. Protect from light. Once reconstituted, store aliquoted at -80°C. Use reconstituted material within 6 months to avoid loss of potency.
Rapamycin was used for induction of Autophagy in a range from 1 to 40 µM. It is recommended that the reagent be titrated for each experiment to determine optimal performance.
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