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ATP Red

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Regulatory Status
RUO
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Product Citations
publications
A. ATP-Red_FC_1_121024
Human peripheral blood mononuclear cells were stimulated with Ultra-LEAF™ Purified anti-human CD3 (clone UCHT1) and Ultra-LEAF™ Purified anti-human CD28 (clone CD28.2) antibodies for 24 hours (filled histogram) or left unstimulated (open histogram) and stained with ATP Red. Data shown were gated on CD3+ lymphocytes.
  • A. ATP-Red_FC_1_121024
    Human peripheral blood mononuclear cells were stimulated with Ultra-LEAF™ Purified anti-human CD3 (clone UCHT1) and Ultra-LEAF™ Purified anti-human CD28 (clone CD28.2) antibodies for 24 hours (filled histogram) or left unstimulated (open histogram) and stained with ATP Red. Data shown were gated on CD3+ lymphocytes.
  • b. ATP-Red_FC_2_121024
    Human peripheral blood mononuclear cells were stimulated Ultra-LEAF™ Purified anti-human CD3 (clone UCHT1) and Ultra-LEAF™ Purified anti-human CD28 (clone CD28.2) antibodies for 24 hours. Cells were then treated with Oligomycin A (an ATP synthase inhibitor) (filled histogram) or left untreated (open histogram) and stained with ATP Red. Data shown were gated on CD3+ lymphocytes.
  • c. ATP-Red_Live-cell_3_121024
    A549 cells grown on coverslips were treated with 2-Deoxy-D-Glucose (2-DG) (right) for 30 minutes at 37°C or left untreated (control) (left) and subsequently stained with ATP Red for 15 minutes. Cells were then washed with PBS and imaged using a 40X objective.
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421944 1 mg £271
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Description

ATP Red is a cell-permeable ATP sensor that localizes to mitochondria and produces fluorescence upon binding to ATP, rapidly responding to fluctuations in mitochondrial ATP levels. It provides a direct and simplified approach to measuring ATP levels in live cells by flow cytometry or live cell imaging. ATP Red allows for the discrimination of rare sub-populations while interrogating important cellular metabolic pathways, such as glycolysis and oxidative phosphorylation. This method is rapid – results can be obtained in less than an hour and yields an accurate measurement of cellular ATP production and its dependency on metabolic pathways of interest.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Formulation
1 vial of lyophilized ATP Red, 1 mg
Preparation
Bring vial to room temperature. To make a 10 mM stock solution, add 178 µL of DMSO to the vial of 1 mg ATP Red and mix thoroughly until completely dissolved.

Note: Store unused ATP Red stock solution in a tightly sealed vial in the dark at room temperature. Stock solution is stable for at least 12 months.
Storage & Handling
2 - 8°C
Application

FC - Quality tested
Live cell imaging - Verified
Recommended Usage

Please refer to the detailed protocol in the application notes section.

Application Notes

Components:
1 vial of 1 mg lyophilized ATP Red, Molar Mass: 561.48 g/mol

Storage:
Upon receipt, store lyophilized probe at 2 - 8°C and protected from light. Upon reconstitution, store unused ATP Red stock solution in a tightly sealed vial in the dark at room temperature. Stock solution is stable for at least 12 months.

Imaging Guidelines:
Ex/Em = 590/620 nm
Fluorescence microscope filter set: Cyanine3/TRITC

Flow Guidelines:
Analysis in PE or similar channel (585/42 or 575/25 or 570/40 filter set)

Protocol:

  1. Grow cells in a tissue culture vessel with a coverslip bottom in desired media or prepare cell suspension containing 0.25 - 2.5 x106 cells in 100 - 500 µL of desired media or Cell Staining Buffer (Cat. No. 420201).
  2. Optional: Fix cells with Fixation Buffer (Cat. No. 420801), if desired.
  3. Add ATP Red at a final concentration of 5 - 10 µM along with desired antibodies.
  4. Incubate cells at room temperature or 37°C for 15 minutes (or up to 30 minutes for live cell imaging).
  5. Optional: Wash cells with PBS or equivalent buffer. If only staining with ATP Red, this washing step can be skipped.
  6. Proceed directly to analysis.

 

Antigen Details

Distribution

Mitochondria

Biology Area
Adaptive Immunity, Cell Biology, Cell Proliferation and Viability, Immunology, Innate Immunity, Mitochondrial Function
Molecular Family
Mitochondrial Markers
Antigen References
  1. Wang L, et al. 2016. Angew Chem Int Ed Engl. 55:1773-6.
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/10/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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