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Human General Phenotyping (26c) Panel

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a. Human-General-Phenotyping_26c_1_040125
T and NK cells
Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. γ/δ T cells are depicted as TCRγ/δ+ CD3+ cells (A, top right). Multiple NK populations were identified as CD3- TCRγ/δ+ HLA-DR- triple-negative cells (B, bottom left). α/β T cells, defined as CD3+ TCRγ/δ- CD56- cells, are further divided into CD4+ and CD8+ T cell populations (C, bottom right). All data were gated on CD45+ viable single cells within the lymphocyte population.
  • a. Human-General-Phenotyping_26c_1_040125
    T and NK cells
    Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. γ/δ T cells are depicted as TCRγ/δ+ CD3+ cells (A, top right). Multiple NK populations were identified as CD3- TCRγ/δ+ HLA-DR- triple-negative cells (B, bottom left). α/β T cells, defined as CD3+ TCRγ/δ- CD56- cells, are further divided into CD4+ and CD8+ T cell populations (C, bottom right). All data were gated on CD45+ viable single cells within the lymphocyte population.
  • b. Human-General-Phenotyping_26c_2_040125
    CD4+ and CD8+ T cells
    Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. CD4+ T cell subsets, including CD25+ CD127- Tregs (A, bottom left) and CD45RA- CCR7- effector memory T cells (TEM) (B, bottom middle) are shown. CD8+ T cell subsets, including CD45RA- CCR7- effector memory T cells (TEM) (C, bottom right) are also shown. All data were gated on CD45+ CD3+ TCRγ/δ- CD56- viable single cells within the lymphocyte population.
  • c. HU-General-Phenotyping_26c_3_040125
    B cells
    Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. CD27+ IgD- memory B cells (A, top right) were further distinguished as CD20- plasmablasts (B, bottom left) and CD20- memory B cells (C, bottom right). All data were gated on CD45+ CD19+ CD3- TCRγ/δ- viable single cells within the lymphocyte population.
  • D. Human-General-Phenotyping_26c_4_040125
    Dendritic cells and monocytes
    Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. Dendritic cells (DCs) were identified as CD45+ HLA-DR+ TCRγ/δ- CD3- CD56- CD19-- cells in the lymphocyte gate (top left). Classical DC subsets are further analyzed (top right and bottom right). Non-classical (CD14-CD16+), intermediate (CD14⁺CD16⁺), and classical (CD14+CD16-) monocytes are shown as viable CD45+ single cells within the monocyte population (bottom left).
  • E. Human-General-Phenotyping_26c_5_040125
    Basophils
    Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. CD45int CD123+ basophils (A) were gated on total viable single cells.
  • F. Human-General-Phenotyping_26c_6_040125
    Innate Lymphoid Cells
    Human peripheral blood mononuclear cells were stained with the Human General Phenotyping (26c) panel. Data shown are gated on CD45+ CD3- TCRγ/δ- CD19- CD20- CD14- CD123- CD16- HLA-DR- cells in the lymphocyte population.
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345907 100 tests 2956€
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Description

The Human General Phenotyping (26 color) Panel has been optimized for examining the major immune cell subsets in human peripheral blood, including T, B, NK, innate lymphoid, myeloid, and dendritic cell subpopulations. This kit includes 25 reagents and a detailed, easy-to-follow protocol for maximal reproducibility. Panel optimization was performed using a 5-laser Cytek® Aurora (UV, V, B, YG, R).

Zombie NIR™ Fixable Viability Kit, (Cat. No. 423105), a required component of the panel, may be purchased separately.
 

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • Human General Phenotyping (26c) Panel Protocol 
  • Anti-human CD11c (clone 3.9) PE/Cyanine7, Cat. No. 301607
  • Anti-human CD45 (clone 2D1) APC/Fire™ 750, Cat. No. 368517  
  • Anti-human CD20 (clone 2H7) Pacific Blue™, Cat. No. 302319
  • Anti-human CD16 (clone 3G8) Brilliant Violet 605™, Cat. No. 302039
  • Anti-human CD56 (NCAM) (clone 5.1H11) Brilliant Violet 750™, Cat. No. 362555
  • Anti-human CD14 (clone 63D3) Spark Blue™ 550, Cat. No. 367147
  • Anti-human CD123 (clone 6H6) Alexa Fluor® 700, Cat. No. 306039
  • Anti-human CD127 (IL-7Rα) (clone A019D5) APC, Cat. No. 351315
  • Anti-human TCR γ/δ (clone B1) PE, Cat. No. 331209
  • Anti-human CD28 (clone CD28.2) Brilliant Violet 650™, Cat. No. 302945
  • Anti-human CD279 (PD-1) (clone EH12.2H7) Brilliant Violet 785™, Cat. No. 329929, 2 vials
  • Anti-human CD197 (CCR7) (clone G043H7), Brilliant Violet 421™, Cat. No. 353207, 2 vials
  • Anti-human CD45RA (clone HI100) Alexa Fluor® 488, Cat. No. 304114
  • Anti-human CD19 (clone HIB19) Spark NIR™ 685, Cat. No. 302269
  • Anti-human CD38 (clone HIT2) Brilliant Violet 711™, Cat. No. 303527
  • Anti-human CD1c (clone L161) Alexa Fluor® 647, Cat. No. 331510
  • Anti-human HLA-DR (clone L243) PE/Fire™ 810, Cat. No. 307683
  • Anti-human CD141 (Thrombomodulin) (clone M80) PE/Dazzle™ 594, Cat. No. 344119
  • Anti-human CD25 (clone M-A251) PE/Fire™ 700, Cat. No. 356145
  • Anti-human IgM (clone MHM-88) Brilliant Violet 510™, Cat. No. 314521
  • Anti-human CD27 (clone O323) APC/Fire™ 810, Cat. No. 302863
  • Anti-human CD8 (clone SK1) PerCP/Cyanine5.5, Cat. No. 344709
  • Anti-human CD4 (clone SK3) Spark Blue™ 574, Cat. No. 344679
  • Anti-human CD3 (clone UCHT1) Brilliant Violet 570™, Cat. No. 300435
  • Anti-human IgD (clone W18340F) Spark YG™ 581, Cat. No. 307805

Materials Not Included

Required Reagents:

  • Zombie NIR™ Fixable Viability Kit, Cat. No. 423105 
  • Phosphate Buffered Saline (PBS) solution (without Ca2+/Mg2+, sodium azide, Tris, or protein) 
  • Cell Staining Buffer, Cat. No. 420201 or equivalent

Recommended Reagents:

  • Compensation Beads, Cat. No. 424602
  • Human TruStain FcX™ (Fc Receptor Blocking Solution), Cat. No. 422302 
  • True Stain Monocyte Blocker ™, Cat. No. 426103 

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Formulation
Varies by component
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The reagents in this kit should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Verified
Recommended Usage

Please see the Human General Phenotyping (26c) Panel Protocol included in the kit. Please contact our technical services team if you have any questions or require assistance.

Application Notes

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  • Experimental results may vary from the representative data shown due to numerous factors including instrument setup, use of additional ancillary reagents, and inherent variability in biological samples.
  • Using a flow cytometer that is compatible with the selected panel is critical for success. Prior to ordering, we urge users to check your flow cytometer specifications to ensure compatibility with the panel.
RRID
AB_3698991 (BioLegend Cat. No. 345907)

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/10/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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