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TMB Substrate Set

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Regulatory Status
RUO
Other Names
TMB Substrate
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421101 110 mL 34€
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Description

The TMB Substrate Set contains 110 ml TMB Substrate A and 110 ml TMB Substrate B, sufficient for twenty 96-well plates. It is formulated with 3, 3', 5, 5' tetramethyl benzidine (TMB). The substrate can be catalyzed with peroxidase to produce a pale blue color which can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with BioLegend's Stop Solution (Cat. No. 423001), and read at 450 nm.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • 1 vial containing 110 mL of TMB Substrate A
  • 1 vial containing 110 mL of TMB Substrate B

Product Details

Host Species
N/A
Product Citations
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RRID
SCR_001134 (BioLegend Cat. No. 421101)

Antigen Details

Gene ID
NA

Related FAQs

What is the difference between your two substrate solutions: TMB Substrate Reagent Set (Cat. No. 421101) and TMB High Sensitivity Substrate Solution (Cat. No. 421501)?

TMB Substrate Reagent Set (Cat. No. 421101) contains two components, which need to be mixed immediately prior to use. The TMB High Sensitivity Substrate Solution (Cat. No. 421501) contains everything in one solution with special stabilizers. It is a high kinetic substrate solution which generally gives higher signal. The type of substrate used for an assay can affect the optical density (OD) achievable and the time required to achieve the desired OD.

Go To Top Version: 0    Revision Date: 01/01/0001

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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