Login/Register 
United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Denmark (DKK) DKK
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Norway (NOK) NOK
Sweden (SEK) SEK
Switzerland (CHF) CHF
United Kingdom (£) GBP
- Regulatory Status
- RUO
- Other Names
- Tumor Necrosis Factor alpha, Cachectin, Tumor necrosis factor ligand superfamily member 2
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 mouse splenocytes (6 x 104 cells/well) were incubated for 20 hours in the absence or presence of 100 ng/mL LPS (lipopolysaccharide). The number of cells secreting Mouse TNF-α was analyzed by ELISpot.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 430924 | 5 plates | $615 | ||||
TNF-α (Tumor Necrosis Factor α), also known as Cachectin, is a cytokine belonging to the Tumor Necrosis Factor superfamily. It is produced by various cell types, including macrophages, monocytes, neutrophils, T cells, NK cells, and epithelial cells. TNF-α can bind to two receptors: TNFR1 and TNFR2. TNF-α signaling plays a central role in the inflammatory response, lymphoid cell development, and lipid metabolism. Additionally, TNF-α is a pathological component in numerous autoimmune diseases, including but not limited to Crohn’s disease, asthma, and rheumatoid arthritis. It was also reported to be involved in some cancers and obesity.
BioLegend’s ELISpot MAX™ Deluxe Set contains pre-optimized essential reagents and additional buffers and solution for Enzyme-Linked Immunosorbent Spot assay. This kit is designed for detection of mouse TNF-α secreting cells. An antibody specific for mouse TNF-α is coated on ethanol-activated 96-well PVDF membrane plate as the capture. Stimulated and/or unstimulated cells are pipetted directly into the wells, and then incubated at 37°C for a specific period of time. During this period, capture antibody binds to mouse TNF-α from the secreting cells in the vicinity. Then, cells are removed, and a biotinylated anti-mouse TNF-α detection antibody is added, followed by Avidin-HRP. Subsequently, TMB membrane substrate is added. The TMB membrane substrate reacts with HRP to form a blue-black precipitate at the sites of mouse TNF-α secreting cells. The end result is visible spots, which correspond to footprints of TNF-α secreting cells, on the membrane surface.
Kit Details
- Kit Contents
-
- Mouse TNF-α ELISpot MAX™ Capture Antibody (200X)
- Mouse TNF-α ELISpot MAX™ Detection Antibody (200X)
- Mouse TNF-α Standard (used as positive control)
- Avidin-HRP (1000X)
- TMB Membrane Substrate
- Coating Buffer A (5X)
- Assay Diluent B (5X)
- Materials Not Included
-
- PVDF-bottom plate: Millipore MultiScreenHTS IP Filter Plate (Catalog No. MSIPS4W10) is recommended.
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- 0.22 μm filters
- 35% Ethanol
- Sterile PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.22 μm filtered
- Sterile cell culture media
- 37°C CO2 incubator
- Deionized (DI) water
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle, manifold dispenser, or automated microplate washer
- Tubes for dilution
- Timer
- Stereomicroscope or an automated ELISpot reader
Product Details
- Verified Reactivity
- Mouse
- Application
-
ELISpot
Antigen Details
- Cell Type
- Macrophages, Neutrophils, NK cells, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity, Neurodegeneration, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Innate Immune Signaling, Tumor Suppressors
- Gene ID
- 21926 View all products for this Gene ID
- UniProt
- View information about TNF-alpha on UniProt.org
Follow Us