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Human CD3/CD28/CD137 T Cell Activation Beads

Pricing & Availability
Regulatory Status
RUO
Other Names
T Cell Stimulation Beads, Human T Cell Stimulation Beads, Human CD3 CD28 CD137 Beads, T cell expansion beads, Memory T cell Expansion Beads
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Product Citations
publications
a. Human-CD3-C28-CD137-T-Cell-Activation-Beads_1_071825
Human CD3+ T cells were isolated from healthy human PBMCs using the MojoSort™ Human CD3 T Cell Isolation Kit (Cat. No. 480022). Isolated cells were left untreated or either expanded in vitro with BioLegend’s Human CD3/CD28/CD137 T Cell Activation Beads or BioLegend’s Human CD3/CD28 T Cell Activation Beads (Cat. No. 422603) at 1:1 particle-to-cell ratio in the absence of human IL-2. On day 7 post-activation with the beads, the number of expanded cells was quantified using AOPI staining. Cell counts for each condition were expressed as a ratio relative to the untreated control.
  • a. Human-CD3-C28-CD137-T-Cell-Activation-Beads_1_071825
    Human CD3+ T cells were isolated from healthy human PBMCs using the MojoSort™ Human CD3 T Cell Isolation Kit (Cat. No. 480022). Isolated cells were left untreated or either expanded in vitro with BioLegend’s Human CD3/CD28/CD137 T Cell Activation Beads or BioLegend’s Human CD3/CD28 T Cell Activation Beads (Cat. No. 422603) at 1:1 particle-to-cell ratio in the absence of human IL-2. On day 7 post-activation with the beads, the number of expanded cells was quantified using AOPI staining. Cell counts for each condition were expressed as a ratio relative to the untreated control.
  • b. Human-CD3-C28-CD137-T-Cell-Activation-Beads_2_071825
    On day 7 after activation with Human CD3/CD28/CD137 T Cell Activation Beads, the expanded T cells were co-stained with PE/Cyanine7 anti-human CD4 (Cat. No. 300511) and Brilliant Violet 510™anti-human CD8a (Cat. No. 301047). Dead cells were excluded by Helix NP™ Blue (Cat. No. 425305) staining. The CD4+ and CD8+ T cells were analyzed by flow cytometry (left panel), and the percentage of CD4-/CD8a+ T cells in the expanded samples was quantified (right panel).
  • c. Human-CD3-C28-CD137-T-Cell-Activation-Beads_3_071825
    On day 7 after activation with Human CD3/CD28/CD137 T Cell Activation Beads, the expanded T cells were stained for flow cytometric analysis. To select for Central Memory T cells (CD8+CCR7+CD45RA-), the cells were initially gated on CD4-CD8+ expression. Then, cells were analyzed with PE/Fire™ 640 anti-human CD197 (CCR7) (Cat. No. 353261) and Spark Red™ 718 anti-human CD45RA (Cat. No. 304183) (left panel). The proportion of Central Memory CD8+ T cells (CD8+CCR7+CD45RA-) was determined (right panel). As a comparison, T cells were also activated with BioLegend’s Human CD3/CD28 T Cell Activation Beads (Cat. No. 422603).
Cat # Size Price Quantity Check Availability Save
422607 0.5 mL $195
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422608 2.5 mL $680
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Description

The Human CD3/CD28/CD137 T Cell Activation Beads allow for convenient in vitro activation of human T cells without the need for antigen-presenting cells or precoating culture plates. Human T cells are stimulated with microbeads coated with monoclonal anti-CD3, anti-CD28 and anti-CD137 antibodies. The antibodies present on the bead surface act as an agonist and stimulate T cells. This mimics antigen presenting cells to induce the proliferation, especially for memory T cells which rely on CD137 signaling for cell maintenance and activation. Activation in vitro with this product results in robust T cell expansion, especially of the central memory (CCR7+ CD45RA-) CD8+ T cell subset.

Simply add the beads directly to isolated T cells at a 1:1 particle-to-cell ratio for robust T cell activation. The antibody-coated beads can be removed from the cells using a magnetic separator such as the MojoSort™ Magnet (Cat. Nos. 480019, 480020, and 480173). Some of the downstream applications include functional assays, gene expression analysis, and phenotypic characterization.

Product Details
Technical data sheet

Kit Details

Kit Contents

For Cat# 422607: 

  • 1 vial containing 0.5 mL of Human CD3/CD28/CD137 T Cell Activation Beads

For Cat# 422608:

  • 1 vial containing 2.5 mL of Human CD3/CD28/CD137 T Cell Activation Beads

Product Details

Verified Reactivity
Human
Formulation
Cocktail: N/A

Particle: Phosphate buffer solution containing 0.1% HSA (azide-free). The particles are at a concentration of 4 x 107 particles/mL.

The Human CD3/CD28/CD137 T Cell Activation Beads are tested for microbial/mycoplasma contamination.
Endotoxin Level
Less than 10 EU/mL as determined by the LAL test.
Preparation
The antibodies were purified by affinity chromatography and conjugated with magnetic microbeads under optimal conditions.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Activ - Quality tested
Recommended Usage

Use Human CD3/CD28/CD137 T Cell Activation beads at a 1:1 particle-to-cell ratio.

Application Notes

This product is designed for the activation of isolated human T cells for research use only (RUO).

The antibody-coated beads can be removed from the cells by the use of a handheld magnetic separator such as the MojoSort™ Magnet (Cat. Nos. 480019, 480020, and 480173) or other commercially available magnetic separators. Please contact BioLegend Technical Service for more product-specific information and guidance.

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/14/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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