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Purified anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
S22006A (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
a. S22006A_PURE_-Ly-6C_FC_030325-2
C57BL/6 mouse splenocytes were stained with purified anti-mouse Ly-6C (clone S22006A) (left) or purified rat IgG1, κ isotype control (right) followed by anti-rat IgG1 PE. Cells were then stained with anti-mouse CD8a (clone S18018E) APC.
  • a. S22006A_PURE_-Ly-6C_FC_030325-2
    C57BL/6 mouse splenocytes were stained with purified anti-mouse Ly-6C (clone S22006A) (left) or purified rat IgG1, κ isotype control (right) followed by anti-rat IgG1 PE. Cells were then stained with anti-mouse CD8a (clone S18018E) APC.
  • b. S22006A_PURE_-Ly-6C_IHC-F_030325
    C57BL/6 mouse frozen spleen sections were treated with Fixation Buffer (Cat. No. 420801) for 10 minutes at room temperature, blocked, and then incubated without (panel A) or with (panel B) 10 µg/mL of purified anti-mouse Ly-6C (clone S22006A) overnight at 4°C. The tissue was then stained with 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 594 (red) and anti-mouse CD3ε (clone 500A2) Alexa Fluor® 647 (green) for two hours at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 60X objective. Scale bar: 100 µm
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168102 100 µg $91
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly6C-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reference clone HK1.4 does not block the staining of clone S22006A.

RRID
AB_3698942 (BioLegend Cat. No. 168102)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cysteine rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Function
After cross linking Ly6C regulate endothelial adhesion of CD8 T cells through activation of LFA-1 at the expense of Ly6C down-regulation on the cell surface
Interaction
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Jutila MA, et al. 1988. Eur J Immunol. 18:1819-26.
  2. Cerwenka A, et al. 1998. J Immunol. 161:97-105.
Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/03/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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