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- Regulatory Status
- RUO
- Other Names
- Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T celldifferentiation factor (CDF), Hepatocyte stimulating factor (HSF),Hybridoma/plasmacytoma growth factor (HPGF)
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- Submit a Review
- Product Citations
- publications
-
Recombinant human IL-6 induces 7TD1 cell (mouse B-cell) proliferation in a dose-dependent manner. ED50 of this effect is 4-20 pg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 712355 | 50 µg | $155 | ||||
Mature human IL-6 is 183 amino acids with 39% aa sequence homology with mouse and rat IL-6. IL-6 is a key immunomodulatory cytokine that affects the pathogenesis of diverse diseases, including autoimmune diseases, chronic inflammatory conditions and cancer. Classical IL-6 signaling involves the binding of IL-6 to the membrane-bound IL-6 receptor α-subunit (hereafter termed ‘mIL-6R’) and glycoprotein 130 (gp130) signal-transducing subunit. Growing understanding of IL-6 biology has led to the description of three distinct modes of IL-6-mediated signaling: ‘classic IL-6 signaling’, ‘IL-6 trans-signaling’ and ‘IL-6 cluster signaling’. In recent years, IL-6 is seen as one of the major immunomodulatory cytokines controlling health and disease. In the tumor microenvironment, IL-6/JAK/STAT3 signaling acts to drive the proliferation, survival, invasiveness and metastasis of tumor cells, while strongly suppressing the antitumor immune response. Thus, treatments that target the IL-6/JAK/STAT3 pathway in patients with cancer provide therapeutic benefit by directly inhibiting tumor cell growth and by stimulating anti-tumor immunity. In addition, pre-clinical and clinical studies have implicated the IL-6 family in many acute and chronic inflammatory disorders (e.g. peritonitis, inflammatory bowel disease, sepsis and atherosclerosis), autoimmune diseases (e.g. rheumatoid arthritis) and glucose homeostasis in obesity.
Product DetailsProduct Details
- Source
- Human IL-6, amino acids Pro29-Met212 (Accession # P05231.1) was expressed in E.coli
- Molecular Mass
- The 185 amino acid recombinant protein has a predicted molecular mass of approximately 21.04 kD. The DTT-reduced and non-reduced protein migrate at approximately 20-25 kD in SDS-PAGE.
-
N-terminal
Sequence Analysis - The predicted N-terminal amino acid is Pro.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- Lyophilized from 0.22 µm filtered protein solution in 20 mM Sodium phosphate, 1M NaCl, pH 7.4 with 5% trehalose.
- Endotoxin Level
- Less than 0.1 EU per μg of protein as determined by the LAL method
- Concentration
- 50 µg size, Lyophilized
- Storage & Handling
- Unopened vial can be stored between 2°C -4°C for up to two weeks and at -20°C or colder until the expiration date. Reconstitute lyophilized protein in 1x sterile PBS. Before reconstitution, make sure 1x sterile PBS and the product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer 1x sterile PBS to the vial, to obtain the recommended stock concentration 250 μg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all of the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 μg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months.
- Activity
- Recombinant human IL-6 induces 7TD1 cell (mouse B-cells) proliferation in dose-dependent manner. ED50 of this effect is 4-20 pg/mL.
- Application
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Bioassay
- Application Notes
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Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
- Function
- Plays role in host defense, acute phase reactions, immune response, inflammation, hematopoiesis, bone metabolism and cancer progression
- Interaction
- T cells, B cells, hepatocytes, cholinergic neurons
- Ligand/Receptor
- Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130, gp130)
- Bioactivity
- Recombinant human IL-6 induces 7TD1 cell (mouse B-cells) proliferation in dose-dependent manner. ED50 of this effect is 4-20 pg/mL.
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Hirano T, et al. 1986. Nature. 324:73-76.
- Jones S and Jenkins B. 2018. Nat Rev Immunol. 18:773-789.
- Stefan RJ, et al. 2023. Nature Reviews Immunology 23:666-681.
- Hirano T, et al. 1986. Nature 324:73.
- Schuett H, et al. 2009. Thromb Haemost. 102:215.
- Dieter Görtz, et al. 2015. Sci Rep. 1:5:14685.
- Prakasam G, et al. 2024. J Clin Invest. 134:e170559.
- Kwon YJ, et al. 2022. BMC Cancer. 22:852.
- Zhou S, et al. 2024. Arch Biochem Biophys. 756:110002.
- Du L, et al. 2020. Life Sci. 258:118217.
- Rashed FE, et al. 2024. Clin Immunol. 2024: 267:110351.
- Timper K, et al. 2017. Cell Rep. 19:267-280.
- Gene ID
- 3569 View all products for this Gene ID
- UniProt
- View information about IL-6 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
-
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
-
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
-
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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