Spark YG™ 581 anti-human CD3 (Flexi-Fluor™) Antibody, CD3, UCHT1

Pricing & Availability

Clone: UCHT1 (See other available formats)
Regulatory Status: RUO
Workshop: III 471
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ
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Product Citations: publications

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285414

50 µg

$394

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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark YG™ 581 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

* Spark YG™ 581 has a maximum excitation of 562 nm and a maximum emission of 581 nm.
Excitation Laser: Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4,6,7 and formalin-fixed paraffin-embedded sections¹¹, immunoprecipitation¹, activation of T cells^2,3,5, Western blotting⁹, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Antigen Details

Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD3 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD3	UCHT1	FC
Biotin anti-human CD3	UCHT1	FC
FITC anti-human CD3	UCHT1	FC
PE anti-human CD3	UCHT1	FC
PE/Cyanine5 anti-human CD3	UCHT1	FC
Purified anti-human CD3	UCHT1	FC,CyTOF®,IHC-F,IP,Activ,WB
Alexa Fluor® 647 anti-human CD3	UCHT1	FC,ICC,IHC-F
Alexa Fluor® 488 anti-human CD3	UCHT1	FC,ICC,IHC-F
Pacific Blue™ anti-human CD3	UCHT1	FC
PE/Cyanine7 anti-human CD3	UCHT1	FC
Alexa Fluor® 700 anti-human CD3	UCHT1	FC
APC/Cyanine7 anti-human CD3	UCHT1	FC
PerCP anti-human CD3	UCHT1	FC
PerCP/Cyanine5.5 anti-human CD3	UCHT1	FC
Brilliant Violet 421™ anti-human CD3	UCHT1	FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD3	UCHT1	FC
Ultra-LEAF™ Purified anti-human CD3	UCHT1	FC,CyTOF®,IHC-F,IP,Activ,WB
Purified anti-human CD3 (Maxpar® Ready)	UCHT1	FC,CyTOF®
Alexa Fluor® 594 anti-human CD3	UCHT1	ICC,FC,SB
PE/Dazzle™ 594 anti-human CD3	UCHT1	FC
Brilliant Violet 510™ anti-human CD3	UCHT1	FC
Brilliant Violet 605™ anti-human CD3	UCHT1	FC
Brilliant Violet 711™ anti-human CD3	UCHT1	FC
Brilliant Violet 650™ anti-human CD3	UCHT1	FC
APC/Fire™ 750 anti-human CD3	UCHT1	FC
Pacific Blue™ anti-human CD3	UCHT1	FC
Brilliant Violet 785™ anti-human CD3	UCHT1	FC
PE/Dazzle™ 594 anti-human CD3	UCHT1	FC
TotalSeq™-A0034 anti-human CD3	UCHT1	PG
TotalSeq™-B0034 anti-human CD3	UCHT1	PG
TotalSeq™-C0034 anti-human CD3	UCHT1	PG
PE anti-human CD3	UCHT1	FC
PE/Cyanine7 anti-human CD3	UCHT1	FC
KIRAVIA Blue 520™ anti-human CD3	UCHT1	FC
Spark Violet™ 538 anti-human CD3 Antibody	UCHT1	FC
TotalSeq™-D0034 anti-human CD3	UCHT1	PG
Spark Blue™ 574 anti-human CD3 Antibody	UCHT1	FC
GMP Pacific Blue™ anti-human CD3	UCHT1	FC
GMP PE anti-human CD3	UCHT1	FC
GMP PE/Dazzle™ 594 anti-human CD3	UCHT1	FC
Spark Violet™ 423 anti-human CD3	UCHT1	FC
GMP PE/Cyanine7 anti-human CD3	UCHT1	FC
Spark Blue™ 515 anti-human CD3	UCHT1	FC
APC/Fire™ 810 anti-human CD3	UCHT1	FC
PE/Fire™ 810 anti-human CD3	UCHT1	FC
Spark YG™ 581 anti-human CD3 (Flexi-Fluor™)	UCHT1	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05/21/2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.