Spark YG™ 593 anti-mouse CD69 (Flexi-Fluor™) Antibody, CD69, H1.2F3

Pricing & Availability

Clone: H1.2F3 (See other available formats)
Regulatory Status: RUO
Other Names: Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Isotype: Armenian Hamster IgG
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285439

50 µg

$336

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Description

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mouse dendritic epidermal T cell line Y245
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark YG™ 593 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

* Spark YG™ 593 has a maximum excitation of 573 nm and a maximum emission of 593 nm.
Excitation Laser: Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation^1-3, immunohistochemistry^4,5, and immunoprecipitation¹.

This antibody has been characterized in the literature as containing a lambda light chain.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Antigen Details

Structure: C-type lectin, 27/33 kD
Distribution: Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets
Function: Lymphocyte activation
Cell Type: B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.
Gene ID: NA
UniProt: View information about CD69 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD69 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse CD69	H1.2F3	FC
FITC anti-mouse CD69	H1.2F3	FC
PE anti-mouse CD69	H1.2F3	FC
PE/Cyanine5 anti-mouse CD69	H1.2F3	FC
Purified anti-mouse CD69	H1.2F3	FC,IHC-F,IP
PE/Cyanine7 anti-mouse CD69	H1.2F3	FC
APC anti-mouse CD69	H1.2F3	FC
Alexa Fluor® 488 anti-mouse CD69	H1.2F3	FC
Alexa Fluor® 647 anti-mouse CD69	H1.2F3	FC
PerCP anti-mouse CD69	H1.2F3	FC
PerCP/Cyanine5.5 anti-mouse CD69	H1.2F3	FC
Pacific Blue™ anti-mouse CD69	H1.2F3	FC
Brilliant Violet 421™ anti-mouse CD69	H1.2F3	FC
APC/Cyanine7 anti-mouse CD69	H1.2F3	FC
Brilliant Violet 605™ anti-mouse CD69	H1.2F3	FC
Brilliant Violet 510™ anti-mouse CD69	H1.2F3	FC
Purified anti-mouse CD69 (Maxpar® Ready)	H1.2F3	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD69	H1.2F3	FC
Brilliant Violet 711™ anti-mouse CD69	H1.2F3	FC
Alexa Fluor® 700 anti-mouse CD69	H1.2F3	FC
Brilliant Violet 650™ anti-mouse CD69	H1.2F3	FC
Brilliant Violet 785™ anti-mouse CD69	H1.2F3	FC
TotalSeq™-A0197 anti-mouse CD69	H1.2F3	PG
APC/Fire™ 750 anti-mouse CD69	H1.2F3	FC
TotalSeq™-C0197 anti-mouse CD69	H1.2F3	PG
TotalSeq™-B0197 anti-mouse CD69	H1.2F3	PG
KIRAVIA Blue 520™ anti-mouse CD69	H1.2F3	FC
Spark NIR™ 685 anti-mouse CD69	H1.2F3	FC
Spark Red™ 718 anti-mouse CD69	H1.2F3	FC
Spark Blue™ 574 anti-mouse CD69 (Flexi-Fluor™)	H1.2F3	FC
Spark PLUS B550™ anti-mouse CD69	H1.2F3	FC
Spark PLUS UV395™ anti-mouse CD69 Antibody	H1.2F3	FC
Spark YG™ 581 anti-mouse CD69 (Flexi-Fluor™)	H1.2F3	FC
Spark YG™ 593 anti-mouse CD69 (Flexi-Fluor™) Antibody	H1.2F3	FC
Spark UV™ 387 anti-mouse CD69 (Flexi-Fluor™)	H1.2F3	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/02/2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.