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PE anti-human TSLPR Antibody

Pricing & Availability
Clone
W20296I (See other available formats)
Regulatory Status
RUO
Other Names
Thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, CRLF2, IL-XR, TSLP-R
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A: W20296I_PE_TSLPR_Antibody_1_032025
Human TSLPR transfected cells were stained with anti-human TSLPR (clone W20296I) PE (filled histogram) or rat IgG2a, κ PE Isotype control (open histogram).
  • A: W20296I_PE_TSLPR_Antibody_1_032025
    Human TSLPR transfected cells were stained with anti-human TSLPR (clone W20296I) PE (filled histogram) or rat IgG2a, κ PE Isotype control (open histogram).
  • B: W20296I_PE_TSLPR_Antibody_2_051625
    PHA stimulated (4 days) human peripheral blood lymphocytes were stained with anti-human CD4 (clone RPA-T4) APC and anti-human TSLPR (clone W20296I) PE (left) or rat IgG2a, κ PE isotype control (right).
  • C: W20296I_PE_TSLPR_Antibody_3_051625
    Human monocyte-derived dendritic cells (induced with GM-CSF, IL-4 and TNF-α for 8 days) were stained with anti-human CD11b (clone ICRF44) APC and anti-human TSLPR (clone W20296I) PE (left) or rat IgG2a, κ PE isotype control (right).
See PE spectral data
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622355 25 tests 105€
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622356 100 tests 228€
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Description

TSLP-R, also known as the thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, and IL-XR, is a type I membrane receptor that forms a functional heterodimeric complex with IL-7R to bind with TSLP. TSLP-R contains a WSXWS motif required for proper protein folding and a box 1 motif important for association with the JAKs. TSLP-R has a predicted molecular weight of approximately 40 kD, and has two isoforms reported that are produced by alternative splicing. The TSLP-R is expressed preferentially in myeloid cells, dendritic cells, and activated monocytes. It is weakly expressed on T cells. Expression has also been reported in heart, skeletal, muscle, and kidney tissues. TSLP binding to the heterodimeric functional receptor (TSLP-R and IL-7R) activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. Ligand receptor interactions have been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells in an allergic response.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human TSLPR
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_3699064 (BioLegend Cat. No. 622355)
AB_3699064 (BioLegend Cat. No. 622356)

Antigen Details

Structure
Type I membrane receptor
Distribution

Expressed preferentially in myeloid cells including dendritic cells and activated monocytes, weakly expressed in T cells; also expressed in heart, skeletal, muscle, and kidney cells.

Function
TSLP binding to functional receptor activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. It has also been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells
Interaction
Forms functional heterodimeric complex with IL-7R to bind TSLP
Ligand/Receptor
TSLP/TSLPR
Cell Type
Dendritic cells, Monocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Reche PA, et al. 2001. J Immunol. 167:336-43. 
  2. Tonozuka Y, et al. 2001. Cytogenet. Cell. Genet. 93:23-5.
  3. Zhang W, et al. 2001. Biochem Biophys Res Commun. 281:878-83.
  4. Wang YH, et al. 2006. Immunity, 24:-838.
Gene ID
64109 View all products for this Gene ID
UniProt
View information about TSLPR on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03.20.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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