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PE anti-Syk Phospho (Tyr296) Recombinant Antibody

Pricing & Availability
Clone
QA20B02 (See other available formats)
Regulatory Status
RUO
Other Names
Spleen Associated Tyrosine Kinase, Spleen Tyrosine Kinase, Tyrosine-Protein Kinase Syk, IMD82
Isotype
Mouse IgG2b, κ
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Product Citations
publications
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949303 25 tests 140€
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949304 100 tests 348€
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Description

Syk is a non-receptor tyrosine kinase that is a member of the Syk family of tyrosine kinases, with broad expression in hematopoietic cells. Upon immune receptor engagement, phosphorylated ITAM motifs within the cytosolic region of the activated receptor become phosphorylated and recruit Syk to the activated receptor via SH2 domains. ITAM-bound Syk is catalytically active, transmitting signals to multiple downstream signaling partners that drives processes such as phagocytosis, proliferation, and differentiation.

Recent evidence also suggests a role for Syk in non-immune cells. Elevated Syk activation is associated with B-cell malignancies, rheumatoid arthritis, and Alzheimer’s disease, and multiple Syk inhibitors are being developed in clinical trials. Syk activity and function are both regulated by tyrosine phosphorylation at multiple sites. Tyr323 and Tyr352 phosphorylation facilitate binding to downstream effectors, and phosphorylation of Tyr525/Tyr526 in the activation loop is required for kinase activity. Tyr296 phosphorylation has been reported in lung epithelial cancer cells, and has been shown to occur as part of an autophosphorylation mechanism in vitro. This site is embedded within a larger N-terminal linker region that is required for nuclear localization in breast cancer cells. Despite rapid autophosphorylation of this site upon Syk activation, it is not required for Syk activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to Syk phosphorylated at tyrosine 296
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

When Syk is phosphorylated at tyrosine 296, the protein may display a slightly higher observed molecular weight by western blot.

RRID
AB_3716900 (BioLegend Cat. No. 949303)
AB_3716900 (BioLegend Cat. No. 949304)

Antigen Details

Structure
Syk isoform A is a 635 amino acid protein with a predicted molecular weight of 72 kD. Syk isoform B is a 612 amino acid protein with a predicted molecular weight of 69.5 kD. Syk isoform B lacks the Tyr296 site.
Distribution

Plasma membrane

Function
B-cell receptor, integrin signaling
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Mansueto MS, et al. 2019. J Biol Chem. 294:7658-7668.
  2. Geahlen RL. 2009. Biochim Biophys Acta. 1793:1115-27.
  3. Taniguchi T, et al. 1991. J Biol Chem. 266:15790-6.
  4. Toyabe S, et al. 2001. Immunology. 103:164-71.
Gene ID
6850 View all products for this Gene ID
UniProt
View information about Syk Phospho on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 10.03.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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