Spark YG™ 593 anti-mouse NK-1.1 (Flexi-Fluor™) Antibody, NK-1.1, PK136

Pricing & Availability

Clone: PK136 (See other available formats)
Regulatory Status: RUO
Other Names: NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype: Mouse IgG2a, κ
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Product Citations: publications

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285452

50 µg

269€

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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NK-1⁺ cells from mouse spleen and bone marrow
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark YG™ 593 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

* Spark YG™ 593 has a maximum excitation of 573 nm and a maximum emission of 593 nm.
Excitation Laser: Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, complement-dependent cytotoxicity³, in vivo depletion^4,5,9,10, mediation of in vitro redirected lysis⁶, blocking of NK cell function⁷, induction of proliferation⁸, immunohistochemical staining of frozen sections¹¹, immunofluorescence microscopy¹¹, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Antigen Details

Structure: NKR-P1 gene family
Distribution: NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)
Function: NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.
Gene ID: 17059 View all products for this Gene ID
UniProt: View information about NK-1.1 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All NK-1.1 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse NK-1.1	PK136	FC
Biotin anti-mouse NK-1.1	PK136	FC
FITC anti-mouse NK-1.1	PK136	FC
PE anti-mouse NK-1.1	PK136	FC
Purified anti-mouse NK-1.1	PK136	FC,CyTOF®,IP,Depletion,Block,IHC-F
PE/Cyanine7 anti-mouse NK-1.1	PK136	FC
PE/Cyanine5 anti-mouse NK-1.1	PK136	FC
Alexa Fluor® 488 anti-mouse NK-1.1	PK136	FC
Alexa Fluor® 647 anti-mouse NK-1.1	PK136	FC
Pacific Blue™ anti-mouse NK-1.1	PK136	FC
Brilliant Violet 711™ anti-mouse NK-1.1	PK136	FC
APC/Cyanine7 anti-mouse NK-1.1	PK136	FC
PerCP anti-mouse NK-1.1	PK136	FC
PerCP/Cyanine5.5 anti-mouse NK-1.1	PK136	FC
Alexa Fluor® 700 anti-mouse NK-1.1	PK136	FC
Brilliant Violet 421™ anti-mouse NK-1.1	PK136	FC,SB
Brilliant Violet 570™ anti-mouse NK-1.1	PK136	FC
Brilliant Violet 650™ anti-mouse NK-1.1	PK136	FC
Brilliant Violet 510™ anti-mouse NK-1.1	PK136	FC
Brilliant Violet 605™ anti-mouse NK-1.1	PK136	FC
Purified anti-mouse NK-1.1 (Maxpar® Ready)	PK136	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse NK-1.1	PK136	FC
Brilliant Violet 785™ anti-mouse NK-1.1	PK136	FC
APC/Fire™ 750 anti-mouse NK-1.1	PK136	FC
TotalSeq™-A0118 anti-mouse NK-1.1	PK136	PG
Ultra-LEAF™ Purified anti-mouse NK-1.1	PK136	FC,CyTOF®,IP,Depletion,Block,IHC-F
TotalSeq™-B0118 anti-mouse NK-1.1	PK136	PG
TotalSeq™-C0118 anti-mouse NK-1.1	PK136	PG
PE/Fire™ 810 anti-mouse NK-1.1	PK136	FC
PE/Fire™ 640 anti-mouse NK-1.1	PK136	FC
Spark UV™ 387 anti-mouse NK-1.1	PK136	FC
PE/Fire™ 700 anti-mouse NK-1.1	PK136	FC
Spark YG™ 593 anti-mouse NK-1.1 (Flexi-Fluor™) Antibody	PK136	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06.02.2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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